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Microbial community analysis reveals high level phylogenetic alterations in the overall gastrointestinal microbiota of diarrhoea-predominant irritable bowel syndrome sufferers.
BMC Gastroenterol 2009; 9:95BG

Abstract

BACKGROUND

A growing amount of scientific evidence suggests that microbes are involved in the aetiology of irritable bowel syndrome (IBS), and the gastrointestinal (GI) microbiota of individuals suffering from diarrhoea-predominant IBS (IBS-D) is distinguishable from other IBS-subtypes. In our study, the GI microbiota of IBS-D patients was evaluated and compared with healthy controls (HC) by using a high-resolution sequencing method. The method allowed microbial community analysis on all levels of microbial genomic guanine plus cytosine (G+C) content, including high G+C bacteria.

METHODS

The collective faecal microbiota composition of ten IBS-D patients was analysed by examining sequences obtained using percent G+C (%G+C) -based profiling and fractioning combined with 16S rRNA gene clone library sequencing of 3267 clones. The IBS-D library was compared with an analogous healthy-control library of 23 subjects. Real-time PCR analysis was used to identify phylotypes belonging to the class Gammaproteobacteria and the order Coriobacteriales.

RESULTS

Significant differences were found between clone libraries of IBS-D patients and controls. The microbial communities of IBS-D patients were enriched in Proteobacteria and Firmicutes, but reduced in the number of Actinobacteria and Bacteroidetes compared to control. In particular, 16S rDNA sequences belonging to the family Lachnospiraceae within the phylum Firmicutes were in greater abundance in the IBS-D clone library.

CONCLUSIONS

In the microbiota of IBS-D sufferers, notable differences were detected among the prominent bacterial phyla (Firmicutes, Actinobacteria, Bacteroidetes, and Proteobacteria) localized within the GI tract.

Authors+Show Affiliations

Department of Basic Veterinary Sciences, Faculty of Veterinary Medicine, PO Box 66, FI-00014 University of Helsinki, Helsinki, Finland. lotta.krogius@helsinki.fiNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20015409

Citation

Krogius-Kurikka, Lotta, et al. "Microbial Community Analysis Reveals High Level Phylogenetic Alterations in the Overall Gastrointestinal Microbiota of Diarrhoea-predominant Irritable Bowel Syndrome Sufferers." BMC Gastroenterology, vol. 9, 2009, p. 95.
Krogius-Kurikka L, Lyra A, Malinen E, et al. Microbial community analysis reveals high level phylogenetic alterations in the overall gastrointestinal microbiota of diarrhoea-predominant irritable bowel syndrome sufferers. BMC Gastroenterol. 2009;9:95.
Krogius-Kurikka, L., Lyra, A., Malinen, E., Aarnikunnas, J., Tuimala, J., Paulin, L., ... Palva, A. (2009). Microbial community analysis reveals high level phylogenetic alterations in the overall gastrointestinal microbiota of diarrhoea-predominant irritable bowel syndrome sufferers. BMC Gastroenterology, 9, p. 95. doi:10.1186/1471-230X-9-95.
Krogius-Kurikka L, et al. Microbial Community Analysis Reveals High Level Phylogenetic Alterations in the Overall Gastrointestinal Microbiota of Diarrhoea-predominant Irritable Bowel Syndrome Sufferers. BMC Gastroenterol. 2009 Dec 17;9:95. PubMed PMID: 20015409.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Microbial community analysis reveals high level phylogenetic alterations in the overall gastrointestinal microbiota of diarrhoea-predominant irritable bowel syndrome sufferers. AU - Krogius-Kurikka,Lotta, AU - Lyra,Anna, AU - Malinen,Erja, AU - Aarnikunnas,Johannes, AU - Tuimala,Jarno, AU - Paulin,Lars, AU - Mäkivuokko,Harri, AU - Kajander,Kajsa, AU - Palva,Airi, Y1 - 2009/12/17/ PY - 2009/07/10/received PY - 2009/12/17/accepted PY - 2009/12/18/entrez PY - 2009/12/18/pubmed PY - 2010/3/24/medline SP - 95 EP - 95 JF - BMC gastroenterology JO - BMC Gastroenterol VL - 9 N2 - BACKGROUND: A growing amount of scientific evidence suggests that microbes are involved in the aetiology of irritable bowel syndrome (IBS), and the gastrointestinal (GI) microbiota of individuals suffering from diarrhoea-predominant IBS (IBS-D) is distinguishable from other IBS-subtypes. In our study, the GI microbiota of IBS-D patients was evaluated and compared with healthy controls (HC) by using a high-resolution sequencing method. The method allowed microbial community analysis on all levels of microbial genomic guanine plus cytosine (G+C) content, including high G+C bacteria. METHODS: The collective faecal microbiota composition of ten IBS-D patients was analysed by examining sequences obtained using percent G+C (%G+C) -based profiling and fractioning combined with 16S rRNA gene clone library sequencing of 3267 clones. The IBS-D library was compared with an analogous healthy-control library of 23 subjects. Real-time PCR analysis was used to identify phylotypes belonging to the class Gammaproteobacteria and the order Coriobacteriales. RESULTS: Significant differences were found between clone libraries of IBS-D patients and controls. The microbial communities of IBS-D patients were enriched in Proteobacteria and Firmicutes, but reduced in the number of Actinobacteria and Bacteroidetes compared to control. In particular, 16S rDNA sequences belonging to the family Lachnospiraceae within the phylum Firmicutes were in greater abundance in the IBS-D clone library. CONCLUSIONS: In the microbiota of IBS-D sufferers, notable differences were detected among the prominent bacterial phyla (Firmicutes, Actinobacteria, Bacteroidetes, and Proteobacteria) localized within the GI tract. SN - 1471-230X UR - https://www.unboundmedicine.com/medline/citation/20015409/Microbial_community_analysis_reveals_high_level_phylogenetic_alterations_in_the_overall_gastrointestinal_microbiota_of_diarrhoea_predominant_irritable_bowel_syndrome_sufferers_ L2 - https://bmcgastroenterol.biomedcentral.com/articles/10.1186/1471-230X-9-95 DB - PRIME DP - Unbound Medicine ER -