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DNA damage in earthworms from highly contaminated soils: assessing resistance to arsenic toxicity by use of the Comet assay.
Mutat Res. 2010 Feb; 696(2):95-100.MR

Abstract

Earthworms native to the former mine site of Devon Great Consols (DGC), UK reside in soils highly contaminated with arsenic (As). These earthworms are considered to have developed a resistance to As toxicity. The mechanisms underlying this resistance however, remain unclear. In the present study, non-resistant, commercially sourced Lumbricus terrestris were exposed to a typical DGC soil in laboratory mesocosms. The earthworms bio-accumulated As from the soil and incurred DNA-damage levels significantly above those observed in the control mesocosm (assessed using the Comet assay). A dose response was observed between DNA damage (% tail DNA) and As concentration in soil (control, 98, 183, 236, 324 and 436mgkg(-1)). As-resistant earthworms (Lumbricus rubellus, Dendrodrilus rubidus and L. terrestris) collected from contaminated soils at DGC (203 to 9025mgkg(-1) As) had also bio-accumulated high levels of As from their host soils, yet demonstrated low levels of DNA damage compared with earthworms from uncontaminated sites. The results demonstrate that the As-contaminated soils at DGC are genotoxic to non-native earthworms and much less so to earthworms native to DGC, thus providing further evidence of an acquired resistance to As toxicity in the native earthworms.

Authors+Show Affiliations

British Geological Survey, Keyworth Nottingham, NG12 5GG UK. drmbutton@gmail.comNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20015476

Citation

Button, Mark, et al. "DNA Damage in Earthworms From Highly Contaminated Soils: Assessing Resistance to Arsenic Toxicity By Use of the Comet Assay." Mutation Research, vol. 696, no. 2, 2010, pp. 95-100.
Button M, Jenkin GR, Bowman KJ, et al. DNA damage in earthworms from highly contaminated soils: assessing resistance to arsenic toxicity by use of the Comet assay. Mutat Res. 2010;696(2):95-100.
Button, M., Jenkin, G. R., Bowman, K. J., Harrington, C. F., Brewer, T. S., Jones, G. D., & Watts, M. J. (2010). DNA damage in earthworms from highly contaminated soils: assessing resistance to arsenic toxicity by use of the Comet assay. Mutation Research, 696(2), 95-100. https://doi.org/10.1016/j.mrgentox.2009.12.009
Button M, et al. DNA Damage in Earthworms From Highly Contaminated Soils: Assessing Resistance to Arsenic Toxicity By Use of the Comet Assay. Mutat Res. 2010;696(2):95-100. PubMed PMID: 20015476.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - DNA damage in earthworms from highly contaminated soils: assessing resistance to arsenic toxicity by use of the Comet assay. AU - Button,Mark, AU - Jenkin,Gawen R T, AU - Bowman,Karen J, AU - Harrington,Chris F, AU - Brewer,Tim S, AU - Jones,George D D, AU - Watts,Michael J, Y1 - 2009/12/14/ PY - 2009/03/31/received PY - 2009/08/06/revised PY - 2009/09/02/accepted PY - 2009/12/18/entrez PY - 2009/12/18/pubmed PY - 2011/9/14/medline SP - 95 EP - 100 JF - Mutation research JO - Mutat Res VL - 696 IS - 2 N2 - Earthworms native to the former mine site of Devon Great Consols (DGC), UK reside in soils highly contaminated with arsenic (As). These earthworms are considered to have developed a resistance to As toxicity. The mechanisms underlying this resistance however, remain unclear. In the present study, non-resistant, commercially sourced Lumbricus terrestris were exposed to a typical DGC soil in laboratory mesocosms. The earthworms bio-accumulated As from the soil and incurred DNA-damage levels significantly above those observed in the control mesocosm (assessed using the Comet assay). A dose response was observed between DNA damage (% tail DNA) and As concentration in soil (control, 98, 183, 236, 324 and 436mgkg(-1)). As-resistant earthworms (Lumbricus rubellus, Dendrodrilus rubidus and L. terrestris) collected from contaminated soils at DGC (203 to 9025mgkg(-1) As) had also bio-accumulated high levels of As from their host soils, yet demonstrated low levels of DNA damage compared with earthworms from uncontaminated sites. The results demonstrate that the As-contaminated soils at DGC are genotoxic to non-native earthworms and much less so to earthworms native to DGC, thus providing further evidence of an acquired resistance to As toxicity in the native earthworms. SN - 0027-5107 UR - https://www.unboundmedicine.com/medline/citation/20015476/DNA_damage_in_earthworms_from_highly_contaminated_soils:_assessing_resistance_to_arsenic_toxicity_by_use_of_the_Comet_assay_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1383-5718(09)00431-8 DB - PRIME DP - Unbound Medicine ER -