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Strategies for the on-line preconcentration and separation of hypolipidaemic drugs using micellar electrokinetic chromatography.
J Chromatogr A. 2010 Jan 15; 1217(3):386-93.JC

Abstract

Three strategies were investigated for the simultaneous separation and on-line preconcentration of charged and neutral hypolipidaemic drugs in micellar electrokinetic chromatography (MEKC). A background electrolyte (BGE) consisting of 20 mM ammonium bicarbonate buffer (pH 8.50) and 50 mM sodium dodecyl sulfate (SDS) was used for the separation and on-line preconcentration of the drugs. The efficiencies of sweeping, analyte focusing by micelle collapse (AFMC), and simultaneous field-amplified sample stacking (FASS) and sweeping, were compared for the preconcentration of eight hypolipidaemic drugs in different conductivity sample matrices. When compared with a hydrodynamic injection (5 s at 50 mbar, 0.51% of capillary volume to detection window) of drug mixture prepared in the separation BGE, improvements of detection sensitivity of 60-, 83-, and 80-fold were obtained with sweeping, AFMC and simultaneous FASS and sweeping, respectively, giving limits of detection (LODs) of 50, 36, and 38 microg/L, respectively. The studied techniques showed suitability for focusing different types of analytes having different values of retention factor (k). This is the first report for the separation of different types of hypolipidaemic drugs by capillary electrophoresis (CE). The three methods were validated then applied for the analysis of target analytes in wastewater samples from Hobart city.

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Authors+Show Affiliations

Dawod M
Australian Centre for Research on Separation Science, School of Chemistry, University of Tasmania, Private Bag 75, Hobart, Tasmania 7001, Australia.
Breadmore MC
No affiliation info available
Guijt RM
No affiliation info available
Haddad PR
No affiliation info available

MeSH

Chemical FractionationChromatography, Micellar Electrokinetic CapillaryHypolipidemic AgentsModels, MolecularReproducibility of ResultsSensitivity and SpecificitySodium Dodecyl Sulfate

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20015504

Citation

Dawod, Mohamed, et al. "Strategies for the On-line Preconcentration and Separation of Hypolipidaemic Drugs Using Micellar Electrokinetic Chromatography." Journal of Chromatography. A, vol. 1217, no. 3, 2010, pp. 386-93.
Dawod M, Breadmore MC, Guijt RM, et al. Strategies for the on-line preconcentration and separation of hypolipidaemic drugs using micellar electrokinetic chromatography. J Chromatogr A. 2010;1217(3):386-93.
Dawod, M., Breadmore, M. C., Guijt, R. M., & Haddad, P. R. (2010). Strategies for the on-line preconcentration and separation of hypolipidaemic drugs using micellar electrokinetic chromatography. Journal of Chromatography. A, 1217(3), 386-93. https://doi.org/10.1016/j.chroma.2009.11.043
Dawod M, et al. Strategies for the On-line Preconcentration and Separation of Hypolipidaemic Drugs Using Micellar Electrokinetic Chromatography. J Chromatogr A. 2010 Jan 15;1217(3):386-93. PubMed PMID: 20015504.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Strategies for the on-line preconcentration and separation of hypolipidaemic drugs using micellar electrokinetic chromatography. AU - Dawod,Mohamed, AU - Breadmore,Michael C, AU - Guijt,Rosanne M, AU - Haddad,Paul R, Y1 - 2009/11/18/ PY - 2009/08/28/received PY - 2009/11/13/revised PY - 2009/11/16/accepted PY - 2009/12/18/entrez PY - 2009/12/18/pubmed PY - 2010/4/21/medline SP - 386 EP - 93 JF - Journal of chromatography. A JO - J Chromatogr A VL - 1217 IS - 3 N2 - Three strategies were investigated for the simultaneous separation and on-line preconcentration of charged and neutral hypolipidaemic drugs in micellar electrokinetic chromatography (MEKC). A background electrolyte (BGE) consisting of 20 mM ammonium bicarbonate buffer (pH 8.50) and 50 mM sodium dodecyl sulfate (SDS) was used for the separation and on-line preconcentration of the drugs. The efficiencies of sweeping, analyte focusing by micelle collapse (AFMC), and simultaneous field-amplified sample stacking (FASS) and sweeping, were compared for the preconcentration of eight hypolipidaemic drugs in different conductivity sample matrices. When compared with a hydrodynamic injection (5 s at 50 mbar, 0.51% of capillary volume to detection window) of drug mixture prepared in the separation BGE, improvements of detection sensitivity of 60-, 83-, and 80-fold were obtained with sweeping, AFMC and simultaneous FASS and sweeping, respectively, giving limits of detection (LODs) of 50, 36, and 38 microg/L, respectively. The studied techniques showed suitability for focusing different types of analytes having different values of retention factor (k). This is the first report for the separation of different types of hypolipidaemic drugs by capillary electrophoresis (CE). The three methods were validated then applied for the analysis of target analytes in wastewater samples from Hobart city. SN - 1873-3778 UR - https://www.unboundmedicine.com/medline/citation/20015504/Strategies_for_the_on_line_preconcentration_and_separation_of_hypolipidaemic_drugs_using_micellar_electrokinetic_chromatography_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0021-9673(09)01704-X DB - PRIME DP - Unbound Medicine ER -

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