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Gas chromatographic-mass spectrometric analysis of volatiles obtained by four different techniques from Salvia rosifolia Sm., and evaluation for biological activity.
J Chromatogr A. 2010 Jan 29; 1217(5):741-8.JC

Abstract

Four different isolation techniques, conventional hydrodistillation (HD), microwave-assisted hydrodistillation (MWHD), microdistillation (MD) and micro-steam distillation-solid-phase microextraction (MSD-SPME), have been used to analyze the volatile constituents from the aerial parts of Salvia rosifolia Sm. by gas chromatography and gas chromatography coupled to mass spectrometry. HD and MWHD techniques produced quantitatively (yield, 0.39% and 0.40%) and qualitatively (aromatic profile) similar essential oils. alpha-Pinene (15.7-34.8%), 1,8-cineole (16.6-25.1%), beta-pinene (6.7-13.5%), beta-caryophyllene (1.4-5.0%) and caryophyllene oxide (1.4-4.4%) were identified as major constituents of this Turkish endemic species. Besides, the hydrodistilled oil of S. rosifolia was evaluated for antibacterial, antifungal, anticancer, antioxidant and cytotoxic activities. The hydrodistilled oil of S. rosifolia showed antibacterial activity against Methicillin-resistant Staphylococcus aureus (MRSA) with a MIC value of 125microg/mL. Other human pathogenic microorganisms (Escherichia coli, Pseudomonas aeruginosa, Enterobacter aerogenes, Salmonella typhimurium, Staphylococcus epidermidis, Candida albicans) were also inhibited within a moderate range (MIC=125-1000microg/mL). Antifungal activity of the oil was also observed against the strawberry anthracnose-causing fungal plant pathogens Colletotrichum acutatum, C. fragariae and C. gloeosporioides. No cytotoxicity was observed for S. rosifolia oil up to 25mg/mL against malignant melanoma, epidermal, ductal and ovary carcinoma.

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Authors+Show Affiliations

Ozek G
Department of Pharmacognosy, Faculty of Pharmacy, Anadolu University, 26470 Eskisehir, Turkey. gozek@anadolu.edu.tr
Demirci F
No affiliation info available
Ozek T
No affiliation info available
Tabanca N
No affiliation info available
Wedge DE
No affiliation info available
Khan SI
No affiliation info available
Başer KH
No affiliation info available
Duran A
No affiliation info available
Hamzaoglu E
No affiliation info available

MeSH

AnimalsBacteriaCell LineCell SurvivalColletotrichumDistillationGas Chromatography-Mass SpectrometryHumansMethicillin-Resistant Staphylococcus aureusMicrobial Sensitivity TestsOils, VolatilePlant Components, AerialPlant ExtractsSalviaSolid Phase Microextraction

Pub Type(s)

Journal Article

Language

eng

PubMed ID

20015509

Citation

Ozek, Gulmira, et al. "Gas Chromatographic-mass Spectrometric Analysis of Volatiles Obtained By Four Different Techniques From Salvia Rosifolia Sm., and Evaluation for Biological Activity." Journal of Chromatography. A, vol. 1217, no. 5, 2010, pp. 741-8.
Ozek G, Demirci F, Ozek T, et al. Gas chromatographic-mass spectrometric analysis of volatiles obtained by four different techniques from Salvia rosifolia Sm., and evaluation for biological activity. J Chromatogr A. 2010;1217(5):741-8.
Ozek, G., Demirci, F., Ozek, T., Tabanca, N., Wedge, D. E., Khan, S. I., Başer, K. H., Duran, A., & Hamzaoglu, E. (2010). Gas chromatographic-mass spectrometric analysis of volatiles obtained by four different techniques from Salvia rosifolia Sm., and evaluation for biological activity. Journal of Chromatography. A, 1217(5), 741-8. https://doi.org/10.1016/j.chroma.2009.11.086
Ozek G, et al. Gas Chromatographic-mass Spectrometric Analysis of Volatiles Obtained By Four Different Techniques From Salvia Rosifolia Sm., and Evaluation for Biological Activity. J Chromatogr A. 2010 Jan 29;1217(5):741-8. PubMed PMID: 20015509.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Gas chromatographic-mass spectrometric analysis of volatiles obtained by four different techniques from Salvia rosifolia Sm., and evaluation for biological activity. AU - Ozek,Gulmira, AU - Demirci,Fatih, AU - Ozek,Temel, AU - Tabanca,Nurhayat, AU - Wedge,David E, AU - Khan,Shabana I, AU - Başer,Kemal Hüsnü Can, AU - Duran,Ahmet, AU - Hamzaoglu,Ergin, Y1 - 2009/12/02/ PY - 2009/06/19/received PY - 2009/10/09/revised PY - 2009/11/27/accepted PY - 2009/12/18/entrez PY - 2009/12/18/pubmed PY - 2010/5/11/medline SP - 741 EP - 8 JF - Journal of chromatography. A JO - J Chromatogr A VL - 1217 IS - 5 N2 - Four different isolation techniques, conventional hydrodistillation (HD), microwave-assisted hydrodistillation (MWHD), microdistillation (MD) and micro-steam distillation-solid-phase microextraction (MSD-SPME), have been used to analyze the volatile constituents from the aerial parts of Salvia rosifolia Sm. by gas chromatography and gas chromatography coupled to mass spectrometry. HD and MWHD techniques produced quantitatively (yield, 0.39% and 0.40%) and qualitatively (aromatic profile) similar essential oils. alpha-Pinene (15.7-34.8%), 1,8-cineole (16.6-25.1%), beta-pinene (6.7-13.5%), beta-caryophyllene (1.4-5.0%) and caryophyllene oxide (1.4-4.4%) were identified as major constituents of this Turkish endemic species. Besides, the hydrodistilled oil of S. rosifolia was evaluated for antibacterial, antifungal, anticancer, antioxidant and cytotoxic activities. The hydrodistilled oil of S. rosifolia showed antibacterial activity against Methicillin-resistant Staphylococcus aureus (MRSA) with a MIC value of 125microg/mL. Other human pathogenic microorganisms (Escherichia coli, Pseudomonas aeruginosa, Enterobacter aerogenes, Salmonella typhimurium, Staphylococcus epidermidis, Candida albicans) were also inhibited within a moderate range (MIC=125-1000microg/mL). Antifungal activity of the oil was also observed against the strawberry anthracnose-causing fungal plant pathogens Colletotrichum acutatum, C. fragariae and C. gloeosporioides. No cytotoxicity was observed for S. rosifolia oil up to 25mg/mL against malignant melanoma, epidermal, ductal and ovary carcinoma. SN - 1873-3778 UR - https://www.unboundmedicine.com/medline/citation/20015509/Gas_chromatographic_mass_spectrometric_analysis_of_volatiles_obtained_by_four_different_techniques_from_Salvia_rosifolia_Sm__and_evaluation_for_biological_activity_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0021-9673(09)01773-7 DB - PRIME DP - Unbound Medicine ER -