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Functional characterization of transient receptor potential channels in mouse urothelial cells.
Am J Physiol Renal Physiol. 2010 Mar; 298(3):F692-701.AJ

Abstract

The bladder urothelium is currently believed to be a sensory structure, contributing to mechano- and chemosensation in the bladder. Transient receptor potential (TRP) cation channels act as polymodal sensors and may underlie some of the receptive properties of urothelial cells. However, the exact TRP channel expression profile of urothelial cells is unclear. In this study, we have performed a systematic analysis of the molecular and functional expression of various TRP channels in mouse urothelium. Urothelial cells from control and trpv4-/- mice were isolated, cultured (12-48 h), and used for quantitative real-time PCR, immunocytochemistry, calcium imaging, and whole cell patch-clamp experiments. At the mRNA level, TRPV4, TRPV2, and TRPM7 were the most abundantly expressed TRP genes. Immunohistochemistry showed a clear expression of TRPV4 in the plasma membrane, whereas TRPV2 was more prominent in the cytoplasm. TRPM7 was detected in the plasma membrane as well as cytoplasmic vesicles. Calcium imaging and patch-clamp experiments using TRP channel agonists and antagonists provided evidence for the functional expression of TRPV4, TRPV2, and TRPM7 but not of TRPA1, TRPV1, and TRPM8. In conclusion, we have demonstrated functional expression of TRPV4, TRPV2, and TRPM7 in mouse urothelial cells. These channels may contribute to the (mechano)sensory function of the urothelial layer and represent potential targets for the treatment of bladder dysfunction.

Authors+Show Affiliations

Department of Molecular Cell Biology, Laboratory Ion Channel Research, Campus Gasthuisberg, Katholieke Universiteit Leuven, Leuven, Belgium. wouter.everaerts@med.kuleuven.beNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20015940

Citation

Everaerts, Wouter, et al. "Functional Characterization of Transient Receptor Potential Channels in Mouse Urothelial Cells." American Journal of Physiology. Renal Physiology, vol. 298, no. 3, 2010, pp. F692-701.
Everaerts W, Vriens J, Owsianik G, et al. Functional characterization of transient receptor potential channels in mouse urothelial cells. Am J Physiol Renal Physiol. 2010;298(3):F692-701.
Everaerts, W., Vriens, J., Owsianik, G., Appendino, G., Voets, T., De Ridder, D., & Nilius, B. (2010). Functional characterization of transient receptor potential channels in mouse urothelial cells. American Journal of Physiology. Renal Physiology, 298(3), F692-701. https://doi.org/10.1152/ajprenal.00599.2009
Everaerts W, et al. Functional Characterization of Transient Receptor Potential Channels in Mouse Urothelial Cells. Am J Physiol Renal Physiol. 2010;298(3):F692-701. PubMed PMID: 20015940.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Functional characterization of transient receptor potential channels in mouse urothelial cells. AU - Everaerts,Wouter, AU - Vriens,Joris, AU - Owsianik,Grzegorz, AU - Appendino,Giovanni, AU - Voets,Thomas, AU - De Ridder,Dirk, AU - Nilius,Bernd, Y1 - 2009/12/16/ PY - 2009/12/18/entrez PY - 2009/12/18/pubmed PY - 2010/3/20/medline SP - F692 EP - 701 JF - American journal of physiology. Renal physiology JO - Am J Physiol Renal Physiol VL - 298 IS - 3 N2 - The bladder urothelium is currently believed to be a sensory structure, contributing to mechano- and chemosensation in the bladder. Transient receptor potential (TRP) cation channels act as polymodal sensors and may underlie some of the receptive properties of urothelial cells. However, the exact TRP channel expression profile of urothelial cells is unclear. In this study, we have performed a systematic analysis of the molecular and functional expression of various TRP channels in mouse urothelium. Urothelial cells from control and trpv4-/- mice were isolated, cultured (12-48 h), and used for quantitative real-time PCR, immunocytochemistry, calcium imaging, and whole cell patch-clamp experiments. At the mRNA level, TRPV4, TRPV2, and TRPM7 were the most abundantly expressed TRP genes. Immunohistochemistry showed a clear expression of TRPV4 in the plasma membrane, whereas TRPV2 was more prominent in the cytoplasm. TRPM7 was detected in the plasma membrane as well as cytoplasmic vesicles. Calcium imaging and patch-clamp experiments using TRP channel agonists and antagonists provided evidence for the functional expression of TRPV4, TRPV2, and TRPM7 but not of TRPA1, TRPV1, and TRPM8. In conclusion, we have demonstrated functional expression of TRPV4, TRPV2, and TRPM7 in mouse urothelial cells. These channels may contribute to the (mechano)sensory function of the urothelial layer and represent potential targets for the treatment of bladder dysfunction. SN - 1522-1466 UR - https://www.unboundmedicine.com/medline/citation/20015940/Functional_characterization_of_transient_receptor_potential_channels_in_mouse_urothelial_cells_ L2 - https://journals.physiology.org/doi/10.1152/ajprenal.00599.2009?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -