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Intraspecies diversity of SARS-like coronaviruses in Rhinolophus sinicus and its implications for the origin of SARS coronaviruses in humans.
J Gen Virol. 2010 Apr; 91(Pt 4):1058-62.JG

Abstract

The Chinese rufous horseshoe bat (Rhinolophus sinicus) has been suggested to carry the direct ancestor of severe acute respiratory syndrome (SARS) coronavirus (SCoV), and the diversity of SARS-like CoVs (SLCoV) within this Rhinolophus species is therefore worth investigating. Here, we demonstrate the remarkable diversity of SLCoVs in R. sinicus and identify a strain with the same pattern of phylogenetic incongruence (i.e. an indication of recombination) as reported previously in another SLCoV strain. Moreover, this strain possesses a distinctive 579 nt deletion in the nsp3 region that was also found in a human SCoV from the late-phase epidemic. Phylogenetic analysis of the Orf1 region suggested that the human SCoVs are phylogenetically closer to SLCoVs in R. sinicus than to SLCoVs in other Rhinolophus species. These findings reveal a closer evolutionary linkage between SCoV in humans and SLCoVs in R. sinicus, defining the scope of surveillance to search for the direct ancestor of human SCoVs.

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Authors+Show Affiliations

Yuan J
State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences (CAS), Wuhan, PR China.
Hon CC
No affiliation info available
Li Y
No affiliation info available
Wang D
No affiliation info available
Xu G
No affiliation info available
Zhang H
No affiliation info available
Zhou P
No affiliation info available
Poon LL
No affiliation info available
Lam TT
No affiliation info available
Leung FC
No affiliation info available
Shi Z
No affiliation info available

MeSH

AnimalsBase SequenceChiropteraCytochromes bHumansMolecular Sequence DataPhylogenyRNA-Dependent RNA PolymeraseRecombination, GeneticSARS VirusViral Nonstructural Proteins

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20016037

Citation

Yuan, Junfa, et al. "Intraspecies Diversity of SARS-like Coronaviruses in Rhinolophus Sinicus and Its Implications for the Origin of SARS Coronaviruses in Humans." The Journal of General Virology, vol. 91, no. Pt 4, 2010, pp. 1058-62.
Yuan J, Hon CC, Li Y, et al. Intraspecies diversity of SARS-like coronaviruses in Rhinolophus sinicus and its implications for the origin of SARS coronaviruses in humans. J Gen Virol. 2010;91(Pt 4):1058-62.
Yuan, J., Hon, C. C., Li, Y., Wang, D., Xu, G., Zhang, H., Zhou, P., Poon, L. L., Lam, T. T., Leung, F. C., & Shi, Z. (2010). Intraspecies diversity of SARS-like coronaviruses in Rhinolophus sinicus and its implications for the origin of SARS coronaviruses in humans. The Journal of General Virology, 91(Pt 4), 1058-62. https://doi.org/10.1099/vir.0.016378-0
Yuan J, et al. Intraspecies Diversity of SARS-like Coronaviruses in Rhinolophus Sinicus and Its Implications for the Origin of SARS Coronaviruses in Humans. J Gen Virol. 2010;91(Pt 4):1058-62. PubMed PMID: 20016037.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Intraspecies diversity of SARS-like coronaviruses in Rhinolophus sinicus and its implications for the origin of SARS coronaviruses in humans. AU - Yuan,Junfa, AU - Hon,Chung-Chau, AU - Li,Yan, AU - Wang,Dingming, AU - Xu,Gelin, AU - Zhang,Huajun, AU - Zhou,Peng, AU - Poon,Leo L M, AU - Lam,Tommy Tsan-Yuk, AU - Leung,Frederick Chi-Ching, AU - Shi,Zhengli, Y1 - 2009/12/16/ PY - 2009/12/18/entrez PY - 2009/12/18/pubmed PY - 2010/4/8/medline SP - 1058 EP - 62 JF - The Journal of general virology JO - J Gen Virol VL - 91 IS - Pt 4 N2 - The Chinese rufous horseshoe bat (Rhinolophus sinicus) has been suggested to carry the direct ancestor of severe acute respiratory syndrome (SARS) coronavirus (SCoV), and the diversity of SARS-like CoVs (SLCoV) within this Rhinolophus species is therefore worth investigating. Here, we demonstrate the remarkable diversity of SLCoVs in R. sinicus and identify a strain with the same pattern of phylogenetic incongruence (i.e. an indication of recombination) as reported previously in another SLCoV strain. Moreover, this strain possesses a distinctive 579 nt deletion in the nsp3 region that was also found in a human SCoV from the late-phase epidemic. Phylogenetic analysis of the Orf1 region suggested that the human SCoVs are phylogenetically closer to SLCoVs in R. sinicus than to SLCoVs in other Rhinolophus species. These findings reveal a closer evolutionary linkage between SCoV in humans and SLCoVs in R. sinicus, defining the scope of surveillance to search for the direct ancestor of human SCoVs. SN - 1465-2099 UR - https://www.unboundmedicine.com/medline/citation/20016037/Intraspecies_diversity_of_SARS_like_coronaviruses_in_Rhinolophus_sinicus_and_its_implications_for_the_origin_of_SARS_coronaviruses_in_humans_ L2 - http://jgv.microbiologyresearch.org/pubmed/content/journal/jgv/10.1099/vir.0.016378-0 DB - PRIME DP - Unbound Medicine ER -