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Preparative isolation and purification of four flavonoids from the petals of Nelumbo nucifera by high-speed counter-current chromatography.
Phytochem Anal. 2010 May-Jun; 21(3):268-72.PA

Abstract

INTRODUCTION

Flavonoids, the primary constituents of the petals of Nelumbo nucifera, are known to have antioxidant properties and antibacterial bioactivities. However, efficient methods for the preparative isolation and purification of flavonoids from this plant are not currently available.

OBJECTIVE

To develop an efficient method for the preparative isolation and purification of flavonoids from the petals of N. nucifera by high-speed counter-current chromatography (HSCCC).

METHODOLOGY

Following an initial clean-up step on a polyamide column, HSCCC was utilised to separate and purify flavonoids. Purities and identities of the isolated compounds were established by HPLC-PAD, ESI-MS, (1)H-NMR and (13)C-NMR.

RESULTS

The separation was performed using a two-phase solvent system composed of ethyl acetate-methanol-water-acetic acid (4 : 1 : 5 : 0.1, by volume), in which the upper phase was used as the stationary phase and the lower phase was used as the mobile phase at a flow-rate of 1.0 mL/min in the head-to-tail elution mode. Ultimately, 5.0 mg syringetin-3-O-beta-d-glucoside, 6.5 mg quercetin-3-O-beta-d-glucoside, 12.8 mg isorhamnetin-3-O-beta-d-glucoside and 32.5 mg kaempferol-3-O-beta-d-glucoside were obtained from 125 mg crude sample.

CONCLUSION

The combination of HSCCC with a polyamide column is an efficient method for the preparative separation and purification of flavonoids from the petals of N. nucifera.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Xingfeng G
College of Food Science and Engineering, Shandong Agricultural University, 61 Daizong Street, Taian, Shandong 271018, People's Republic of China.
Daijie W
No affiliation info available
Wenjuan D
No affiliation info available
Jinhua D
No affiliation info available
Xiao W
No affiliation info available

MeSH

Countercurrent DistributionFlavonoidsFlowersMagnetic Resonance SpectroscopyMolecular StructureNelumboNylonsReproducibility of ResultsSpectrometry, Mass, Electrospray Ionization

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20020439

Citation

Xingfeng, Guo, et al. "Preparative Isolation and Purification of Four Flavonoids From the Petals of Nelumbo Nucifera By High-speed Counter-current Chromatography." Phytochemical Analysis : PCA, vol. 21, no. 3, 2010, pp. 268-72.
Xingfeng G, Daijie W, Wenjuan D, et al. Preparative isolation and purification of four flavonoids from the petals of Nelumbo nucifera by high-speed counter-current chromatography. Phytochem Anal. 2010;21(3):268-72.
Xingfeng, G., Daijie, W., Wenjuan, D., Jinhua, D., & Xiao, W. (2010). Preparative isolation and purification of four flavonoids from the petals of Nelumbo nucifera by high-speed counter-current chromatography. Phytochemical Analysis : PCA, 21(3), 268-72. https://doi.org/10.1002/pca.1196
Xingfeng G, et al. Preparative Isolation and Purification of Four Flavonoids From the Petals of Nelumbo Nucifera By High-speed Counter-current Chromatography. Phytochem Anal. 2010 May-Jun;21(3):268-72. PubMed PMID: 20020439.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Preparative isolation and purification of four flavonoids from the petals of Nelumbo nucifera by high-speed counter-current chromatography. AU - Xingfeng,Guo, AU - Daijie,Wang, AU - Wenjuan,Duan, AU - Jinhua,Du, AU - Xiao,Wang, PY - 2009/12/19/entrez PY - 2009/12/19/pubmed PY - 2010/8/11/medline SP - 268 EP - 72 JF - Phytochemical analysis : PCA JO - Phytochem Anal VL - 21 IS - 3 N2 - INTRODUCTION: Flavonoids, the primary constituents of the petals of Nelumbo nucifera, are known to have antioxidant properties and antibacterial bioactivities. However, efficient methods for the preparative isolation and purification of flavonoids from this plant are not currently available. OBJECTIVE: To develop an efficient method for the preparative isolation and purification of flavonoids from the petals of N. nucifera by high-speed counter-current chromatography (HSCCC). METHODOLOGY: Following an initial clean-up step on a polyamide column, HSCCC was utilised to separate and purify flavonoids. Purities and identities of the isolated compounds were established by HPLC-PAD, ESI-MS, (1)H-NMR and (13)C-NMR. RESULTS: The separation was performed using a two-phase solvent system composed of ethyl acetate-methanol-water-acetic acid (4 : 1 : 5 : 0.1, by volume), in which the upper phase was used as the stationary phase and the lower phase was used as the mobile phase at a flow-rate of 1.0 mL/min in the head-to-tail elution mode. Ultimately, 5.0 mg syringetin-3-O-beta-d-glucoside, 6.5 mg quercetin-3-O-beta-d-glucoside, 12.8 mg isorhamnetin-3-O-beta-d-glucoside and 32.5 mg kaempferol-3-O-beta-d-glucoside were obtained from 125 mg crude sample. CONCLUSION: The combination of HSCCC with a polyamide column is an efficient method for the preparative separation and purification of flavonoids from the petals of N. nucifera. SN - 1099-1565 UR - https://www.unboundmedicine.com/medline/citation/20020439/Preparative_isolation_and_purification_of_four_flavonoids_from_the_petals_of_Nelumbo_nucifera_by_high_speed_counter_current_chromatography_ L2 - https://doi.org/10.1002/pca.1196 DB - PRIME DP - Unbound Medicine ER -