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Performance of laboratory diagnostics for the detection of influenza A(H1N1)v virus as correlated with the time after symptom onset and viral load.
J Clin Virol. 2010 Feb; 47(2):182-5.JC

Abstract

BACKGROUND

To diagnose influenza A(H1N1)v virus infection, accurate and rapid detection are important. However, there is scanty data on the performance of various laboratory diagnostics.

OBJECTIVE

To compare the performance of rapid antigen test (RAT), viral culture and RT-PCR for the detection of influenza A(H1N1)v virus and to correlate their performance with the time after symptom onset and viral load.

STUDY DESIGN

From May 1, 2009 to June 25, 2009, respiratory samples were collected from 5740 individuals suspected of having influenza A(H1N1)v infection. The performance of viral culture and RT-PCR were investigated and correlated with the time after symptom onset. The sensitivity of RAT ESPLINE influenza A & B-N (Fujirebio Inc, Tokyo) was evaluated using a subset of 60 samples from patients diagnosed as having influenza A(H1N1)v infection.

RESULTS

Using respiratory samples from 587 patients diagnosed with influenza A(H1N1)v infection, comparison of laboratory diagnostics showed viral culture and RT-PCR gave comparable results with overall sensitivity of 93.9% and 98.1%, respectively. For RAT, when testing a subset of 60 samples collected < or =3 days following symptom onset, the sensitivity was 62%.

CONCLUSIONS

Although viral shedding is prolonged and of higher titre in influenza A(H1N1)v infection, RAT showed a low sensitivity of 62% among patients presenting < or =3 days after symptom onset. Viral culture showed comparable performance with RT-PCR and with sensitivity better than that documented for seasonal influenza.

Authors+Show Affiliations

Centre for Health Protection, Department of Health, Hong Kong, People's Republic of China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Evaluation Study
Journal Article

Language

eng

PubMed ID

20022296

Citation

Cheng, Peter K C., et al. "Performance of Laboratory Diagnostics for the Detection of Influenza A(H1N1)v Virus as Correlated With the Time After Symptom Onset and Viral Load." Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology, vol. 47, no. 2, 2010, pp. 182-5.
Cheng PK, Wong KK, Mak GC, et al. Performance of laboratory diagnostics for the detection of influenza A(H1N1)v virus as correlated with the time after symptom onset and viral load. J Clin Virol. 2010;47(2):182-5.
Cheng, P. K., Wong, K. K., Mak, G. C., Wong, A. H., Ng, A. Y., Chow, S. Y., Lam, R. K., Lau, C. S., Ng, K. C., & Lim, W. (2010). Performance of laboratory diagnostics for the detection of influenza A(H1N1)v virus as correlated with the time after symptom onset and viral load. Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology, 47(2), 182-5. https://doi.org/10.1016/j.jcv.2009.11.022
Cheng PK, et al. Performance of Laboratory Diagnostics for the Detection of Influenza A(H1N1)v Virus as Correlated With the Time After Symptom Onset and Viral Load. J Clin Virol. 2010;47(2):182-5. PubMed PMID: 20022296.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Performance of laboratory diagnostics for the detection of influenza A(H1N1)v virus as correlated with the time after symptom onset and viral load. AU - Cheng,Peter K C, AU - Wong,Kitty K Y, AU - Mak,Gannon C, AU - Wong,Ann H, AU - Ng,Anita Y Y, AU - Chow,Salina Y K, AU - Lam,Ricky K H, AU - Lau,C S, AU - Ng,K C, AU - Lim,Wilina, PY - 2009/08/14/received PY - 2009/11/08/revised PY - 2009/11/19/accepted PY - 2009/12/22/entrez PY - 2009/12/22/pubmed PY - 2010/4/8/medline SP - 182 EP - 5 JF - Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology JO - J. Clin. Virol. VL - 47 IS - 2 N2 - BACKGROUND: To diagnose influenza A(H1N1)v virus infection, accurate and rapid detection are important. However, there is scanty data on the performance of various laboratory diagnostics. OBJECTIVE: To compare the performance of rapid antigen test (RAT), viral culture and RT-PCR for the detection of influenza A(H1N1)v virus and to correlate their performance with the time after symptom onset and viral load. STUDY DESIGN: From May 1, 2009 to June 25, 2009, respiratory samples were collected from 5740 individuals suspected of having influenza A(H1N1)v infection. The performance of viral culture and RT-PCR were investigated and correlated with the time after symptom onset. The sensitivity of RAT ESPLINE influenza A & B-N (Fujirebio Inc, Tokyo) was evaluated using a subset of 60 samples from patients diagnosed as having influenza A(H1N1)v infection. RESULTS: Using respiratory samples from 587 patients diagnosed with influenza A(H1N1)v infection, comparison of laboratory diagnostics showed viral culture and RT-PCR gave comparable results with overall sensitivity of 93.9% and 98.1%, respectively. For RAT, when testing a subset of 60 samples collected < or =3 days following symptom onset, the sensitivity was 62%. CONCLUSIONS: Although viral shedding is prolonged and of higher titre in influenza A(H1N1)v infection, RAT showed a low sensitivity of 62% among patients presenting < or =3 days after symptom onset. Viral culture showed comparable performance with RT-PCR and with sensitivity better than that documented for seasonal influenza. SN - 1873-5967 UR - https://www.unboundmedicine.com/medline/citation/20022296/Performance_of_laboratory_diagnostics_for_the_detection_of_influenza_A_H1N1_v_virus_as_correlated_with_the_time_after_symptom_onset_and_viral_load_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1386-6532(09)00563-0 DB - PRIME DP - Unbound Medicine ER -