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Direct identification of phenolic constituents in Boldo Folium (Peumus boldus Mol.) infusions by high-performance liquid chromatography with diode array detection and electrospray ionization tandem mass spectrometry.
J Chromatogr A. 2010 Jan 22; 1217(4):443-9.JC

Abstract

A very simple and direct method was developed for the qualitative analysis of polyphenols in boldo (Peumus boldus Mol., Monimiaceae) leaves infusions by high-performance liquid chromatography with diode array detection (HPLC-DAD) and electrospray ionization tandem mass spectrometry (HPLC-MS(n)). The phenolic constituents identified in infusions of the crude drug Boldo Folium were mainly proanthocyanidins and flavonol glycosides. In the infusions, 41 compounds were detected in male and 43 compounds in female leaf samples, respectively. Nine quercetin glycosides, eight kaempferol derivatives, nine isorhamnetin glycosides, three phenolic acids, one caffeoylquinic acid glycoside and twenty one proanthocyanidins were identified by HPLC-DAD and ESI-MS for the first time in the crude drug. Isorhamnetin glucosyl-di-rhamnoside was the most abundant flavonol glycoside in the male boldo sample, whereas isorhamnetin di-glucosyl-di-rhamnoside was the main phenolic compound in female boldo leaves infusion. The results suggest that the medicinal properties reported for this popular infusion should be attributed not only to the presence of catechin and boldine but also to several phenolic compounds with known antioxidant activity. The HPLC fingerprint obtained can be useful in the authentication of the crude drug Boldo Folium as well as for qualitative analysis and differentiation of plant populations in the tree distribution range.

Authors+Show Affiliations

Laboratorio de Química de Productos Naturales, Instituto de Química de Recursos Naturales, Universidad de Talca, Casilla 747, Talca, Chile.No affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

20022332

Citation

Simirgiotis, M J., and G Schmeda-Hirschmann. "Direct Identification of Phenolic Constituents in Boldo Folium (Peumus Boldus Mol.) Infusions By High-performance Liquid Chromatography With Diode Array Detection and Electrospray Ionization Tandem Mass Spectrometry." Journal of Chromatography. A, vol. 1217, no. 4, 2010, pp. 443-9.
Simirgiotis MJ, Schmeda-Hirschmann G. Direct identification of phenolic constituents in Boldo Folium (Peumus boldus Mol.) infusions by high-performance liquid chromatography with diode array detection and electrospray ionization tandem mass spectrometry. J Chromatogr A. 2010;1217(4):443-9.
Simirgiotis, M. J., & Schmeda-Hirschmann, G. (2010). Direct identification of phenolic constituents in Boldo Folium (Peumus boldus Mol.) infusions by high-performance liquid chromatography with diode array detection and electrospray ionization tandem mass spectrometry. Journal of Chromatography. A, 1217(4), 443-9. https://doi.org/10.1016/j.chroma.2009.11.014
Simirgiotis MJ, Schmeda-Hirschmann G. Direct Identification of Phenolic Constituents in Boldo Folium (Peumus Boldus Mol.) Infusions By High-performance Liquid Chromatography With Diode Array Detection and Electrospray Ionization Tandem Mass Spectrometry. J Chromatogr A. 2010 Jan 22;1217(4):443-9. PubMed PMID: 20022332.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Direct identification of phenolic constituents in Boldo Folium (Peumus boldus Mol.) infusions by high-performance liquid chromatography with diode array detection and electrospray ionization tandem mass spectrometry. AU - Simirgiotis,M J, AU - Schmeda-Hirschmann,G, Y1 - 2009/12/04/ PY - 2009/08/26/received PY - 2009/10/14/revised PY - 2009/11/02/accepted PY - 2009/12/22/entrez PY - 2009/12/22/pubmed PY - 2010/4/7/medline SP - 443 EP - 9 JF - Journal of chromatography. A JO - J Chromatogr A VL - 1217 IS - 4 N2 - A very simple and direct method was developed for the qualitative analysis of polyphenols in boldo (Peumus boldus Mol., Monimiaceae) leaves infusions by high-performance liquid chromatography with diode array detection (HPLC-DAD) and electrospray ionization tandem mass spectrometry (HPLC-MS(n)). The phenolic constituents identified in infusions of the crude drug Boldo Folium were mainly proanthocyanidins and flavonol glycosides. In the infusions, 41 compounds were detected in male and 43 compounds in female leaf samples, respectively. Nine quercetin glycosides, eight kaempferol derivatives, nine isorhamnetin glycosides, three phenolic acids, one caffeoylquinic acid glycoside and twenty one proanthocyanidins were identified by HPLC-DAD and ESI-MS for the first time in the crude drug. Isorhamnetin glucosyl-di-rhamnoside was the most abundant flavonol glycoside in the male boldo sample, whereas isorhamnetin di-glucosyl-di-rhamnoside was the main phenolic compound in female boldo leaves infusion. The results suggest that the medicinal properties reported for this popular infusion should be attributed not only to the presence of catechin and boldine but also to several phenolic compounds with known antioxidant activity. The HPLC fingerprint obtained can be useful in the authentication of the crude drug Boldo Folium as well as for qualitative analysis and differentiation of plant populations in the tree distribution range. SN - 1873-3778 UR - https://www.unboundmedicine.com/medline/citation/20022332/Direct_identification_of_phenolic_constituents_in_Boldo_Folium__Peumus_boldus_Mol___infusions_by_high_performance_liquid_chromatography_with_diode_array_detection_and_electrospray_ionization_tandem_mass_spectrometry_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0021-9673(09)01652-5 DB - PRIME DP - Unbound Medicine ER -