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CXCR4 positive bone mesenchymal stem cells migrate to human endothelial cell stimulated by ox-LDL via SDF-1alpha/CXCR4 signaling axis.
Exp Mol Pathol. 2010 Apr; 88(2):250-5.EM

Abstract

BACKGROUND

Bone mesenchymal stem cells (BMSCs) are attractive candidates for cell based therapies to cardiovascular disease such as infarction and atherosclerosis; however, the mechanisms responsible for stem cell chemotaxis and homing remain unknown. Chemokine stromal cell-derived factor 1 (SDF-1alpha) is involved in the process of atherogenesis. This study was aimed at investigating whether the SDF-1alpha of human umbilical vein endothelial cells (HUVECs) plays a role in migration of BM-derived CXCR4(+)(receptor for SDF-1alpha) stem cells.

METHODS

HUVECs were cultured from human umbilical cords and was treated with ox-LDL. The mRNA and protein expression of SDF-1alpha was detected in HUVECs. CXCR4(+)BMSCs from bone marrow were isolated and were tested by migration and adhesion assays.

RESULTS

It was found that ox-LDL induced HUVECs to increase the mRNA and protein expression of SDF-1alpha. Ox-LDL increased the migratory and adhesion response of CXCR4(+)BMSCs. When the neutralizing SDF-1alpha antibody abrogated the secreted SDF-1alpha, the migration and adhesion response of CXCR4(+)BMSCs markedly decreased.

CONCLUSIONS

Our data indicated that the endothelial cells (ECs) stimulated by ox-LDL could increase the BMSCs migratory response via SDF-1alpha/CXCR4 signaling axis. These findings provide a new paradigm for biological effects of ox-LDL and have implications for novel stem cell therapeutic strategies for atherosclerosis.

Authors+Show Affiliations

Institute of Pathology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, 13 Hangkong Road, Wuhan 430030, PR China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20025867

Citation

Li, Mincai, et al. "CXCR4 Positive Bone Mesenchymal Stem Cells Migrate to Human Endothelial Cell Stimulated By ox-LDL Via SDF-1alpha/CXCR4 Signaling Axis." Experimental and Molecular Pathology, vol. 88, no. 2, 2010, pp. 250-5.
Li M, Yu J, Li Y, et al. CXCR4 positive bone mesenchymal stem cells migrate to human endothelial cell stimulated by ox-LDL via SDF-1alpha/CXCR4 signaling axis. Exp Mol Pathol. 2010;88(2):250-5.
Li, M., Yu, J., Li, Y., Li, D., Yan, D., Qu, Z., & Ruan, Q. (2010). CXCR4 positive bone mesenchymal stem cells migrate to human endothelial cell stimulated by ox-LDL via SDF-1alpha/CXCR4 signaling axis. Experimental and Molecular Pathology, 88(2), 250-5. https://doi.org/10.1016/j.yexmp.2009.12.001
Li M, et al. CXCR4 Positive Bone Mesenchymal Stem Cells Migrate to Human Endothelial Cell Stimulated By ox-LDL Via SDF-1alpha/CXCR4 Signaling Axis. Exp Mol Pathol. 2010;88(2):250-5. PubMed PMID: 20025867.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - CXCR4 positive bone mesenchymal stem cells migrate to human endothelial cell stimulated by ox-LDL via SDF-1alpha/CXCR4 signaling axis. AU - Li,Mincai, AU - Yu,Jun, AU - Li,Yan, AU - Li,Dujuan, AU - Yan,Dan, AU - Qu,Zhiling, AU - Ruan,Qiurong, Y1 - 2009/12/16/ PY - 2009/10/14/received PY - 2009/12/01/revised PY - 2009/12/01/accepted PY - 2009/12/23/entrez PY - 2009/12/23/pubmed PY - 2010/4/24/medline SP - 250 EP - 5 JF - Experimental and molecular pathology JO - Exp Mol Pathol VL - 88 IS - 2 N2 - BACKGROUND: Bone mesenchymal stem cells (BMSCs) are attractive candidates for cell based therapies to cardiovascular disease such as infarction and atherosclerosis; however, the mechanisms responsible for stem cell chemotaxis and homing remain unknown. Chemokine stromal cell-derived factor 1 (SDF-1alpha) is involved in the process of atherogenesis. This study was aimed at investigating whether the SDF-1alpha of human umbilical vein endothelial cells (HUVECs) plays a role in migration of BM-derived CXCR4(+)(receptor for SDF-1alpha) stem cells. METHODS: HUVECs were cultured from human umbilical cords and was treated with ox-LDL. The mRNA and protein expression of SDF-1alpha was detected in HUVECs. CXCR4(+)BMSCs from bone marrow were isolated and were tested by migration and adhesion assays. RESULTS: It was found that ox-LDL induced HUVECs to increase the mRNA and protein expression of SDF-1alpha. Ox-LDL increased the migratory and adhesion response of CXCR4(+)BMSCs. When the neutralizing SDF-1alpha antibody abrogated the secreted SDF-1alpha, the migration and adhesion response of CXCR4(+)BMSCs markedly decreased. CONCLUSIONS: Our data indicated that the endothelial cells (ECs) stimulated by ox-LDL could increase the BMSCs migratory response via SDF-1alpha/CXCR4 signaling axis. These findings provide a new paradigm for biological effects of ox-LDL and have implications for novel stem cell therapeutic strategies for atherosclerosis. SN - 1096-0945 UR - https://www.unboundmedicine.com/medline/citation/20025867/CXCR4_positive_bone_mesenchymal_stem_cells_migrate_to_human_endothelial_cell_stimulated_by_ox_LDL_via_SDF_1alpha/CXCR4_signaling_axis_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0014-4800(09)00167-1 DB - PRIME DP - Unbound Medicine ER -