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Gastroprotective effect of barbatusin and 3-beta-hydroxy-3-deoxibarbatusin, quinonoid diterpenes isolated from Plectranthus grandis, in ethanol-induced gastric lesions in mice.
J Ethnopharmacol. 2010 Feb 17; 127(3):725-30.JE

Abstract

ETHNOPHARMACOLOGICAL RELEVANCE

Validate the popular use of Plectranthus grandis in gastric disorders through the active components.

AIMS

Isolation of barbatusin (BB) and 3beta-hydroxy-3-deoxibarbatusin (BBOH), diterpenes from Plectranthus grandis, and evaluation of their gastroprotective effect and possible mechanisms of action.

MATERIALS AND METHODS

Isolation and chemical characterization of diterpenes from Plectranthus grandis by chromatographic and spectroscopic methods and evaluation of gastroprotective action of the diterpenes through ethanol-induced gastric injury in mice model. It was evaluated the effect of capsazepine, indomethacin and the role of nitric oxide and K(ATP-) channels on the gastroprotective effect of BBOH and BB. Additionally it was measured the concentrations of gastric mucus, non-proteic-sulfhydryl groups and total thiobarbituric acid-reactive substances.

RESULTS

Orally administered BBOH and BB at doses of 5 and 10mg/kg, markedly reduced the gastric lesions by 59 and 96%, and 32 and 76%, respectively, with superior results as compared to N-acetylcysteine (150 mg/kg, i.p.), reference compound that caused 85% lesion suppression. Although BBOH presented a higher gastroprotection than BB they act by similar mechanisms in relation to N-acetylcysteine, and prevent the depletion of gastric mucus, gastric mucosal non-proteic-sulfhydryl groups as well as the increase in thiobarbituric acid-reactive species. Moreover, the gastroprotective effect of BB was effectively blocked in mice pretreated with TRPV1 antagonist capsazepine, by the non-selective cyclooxygenase inhibitor indomethacin, or by the nitric oxide synthase inhibitor L-NAME but not by K(+)(ATP) channel inhibitor glibenclamide. In contrast, the gastroprotective effect of BBOH was blocked only by indomethacin and glibenclamide pretreatments.

CONCLUSION

The protective role for BBOH and BB affording gastroprotection against gastric damage induced by ethanol indicates that these compounds contribute for the activity of Plectranthus species. The different modes of action are probably related to differences in their chemical structure.

Authors+Show Affiliations

State University of Ceará, Post-Graduate Programme of Veterinary Sciences, Veterinary Faculty, Fortaleza, CE, Brazil.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20025953

Citation

Rodrigues, Patrícia de Araújo, et al. "Gastroprotective Effect of Barbatusin and 3-beta-hydroxy-3-deoxibarbatusin, Quinonoid Diterpenes Isolated From Plectranthus Grandis, in Ethanol-induced Gastric Lesions in Mice." Journal of Ethnopharmacology, vol. 127, no. 3, 2010, pp. 725-30.
Rodrigues Pde A, de Morais SM, de Souza CM, et al. Gastroprotective effect of barbatusin and 3-beta-hydroxy-3-deoxibarbatusin, quinonoid diterpenes isolated from Plectranthus grandis, in ethanol-induced gastric lesions in mice. J Ethnopharmacol. 2010;127(3):725-30.
Rodrigues, P. d. e. . A., de Morais, S. M., de Souza, C. M., Silva, A. R., de Andrade, G. M., Silva, M. G., Albuquerque, R. L., Rao, V. S., & Santos, F. A. (2010). Gastroprotective effect of barbatusin and 3-beta-hydroxy-3-deoxibarbatusin, quinonoid diterpenes isolated from Plectranthus grandis, in ethanol-induced gastric lesions in mice. Journal of Ethnopharmacology, 127(3), 725-30. https://doi.org/10.1016/j.jep.2009.11.031
Rodrigues Pde A, et al. Gastroprotective Effect of Barbatusin and 3-beta-hydroxy-3-deoxibarbatusin, Quinonoid Diterpenes Isolated From Plectranthus Grandis, in Ethanol-induced Gastric Lesions in Mice. J Ethnopharmacol. 2010 Feb 17;127(3):725-30. PubMed PMID: 20025953.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Gastroprotective effect of barbatusin and 3-beta-hydroxy-3-deoxibarbatusin, quinonoid diterpenes isolated from Plectranthus grandis, in ethanol-induced gastric lesions in mice. AU - Rodrigues,Patrícia de Araújo, AU - de Morais,Selene Maia, AU - de Souza,Carolina Melo, AU - Silva,Ana Raquel Araújo, AU - de Andrade,Geanne Matos, AU - Silva,Maria Goretti Vasconcelos, AU - Albuquerque,Roberto Lima, AU - Rao,Vietla Satyanarayana, AU - Santos,Flávia Almeida, Y1 - 2009/12/16/ PY - 2009/06/24/received PY - 2009/11/25/revised PY - 2009/11/30/accepted PY - 2009/12/23/entrez PY - 2009/12/23/pubmed PY - 2010/8/7/medline SP - 725 EP - 30 JF - Journal of ethnopharmacology JO - J Ethnopharmacol VL - 127 IS - 3 N2 - ETHNOPHARMACOLOGICAL RELEVANCE: Validate the popular use of Plectranthus grandis in gastric disorders through the active components. AIMS: Isolation of barbatusin (BB) and 3beta-hydroxy-3-deoxibarbatusin (BBOH), diterpenes from Plectranthus grandis, and evaluation of their gastroprotective effect and possible mechanisms of action. MATERIALS AND METHODS: Isolation and chemical characterization of diterpenes from Plectranthus grandis by chromatographic and spectroscopic methods and evaluation of gastroprotective action of the diterpenes through ethanol-induced gastric injury in mice model. It was evaluated the effect of capsazepine, indomethacin and the role of nitric oxide and K(ATP-) channels on the gastroprotective effect of BBOH and BB. Additionally it was measured the concentrations of gastric mucus, non-proteic-sulfhydryl groups and total thiobarbituric acid-reactive substances. RESULTS: Orally administered BBOH and BB at doses of 5 and 10mg/kg, markedly reduced the gastric lesions by 59 and 96%, and 32 and 76%, respectively, with superior results as compared to N-acetylcysteine (150 mg/kg, i.p.), reference compound that caused 85% lesion suppression. Although BBOH presented a higher gastroprotection than BB they act by similar mechanisms in relation to N-acetylcysteine, and prevent the depletion of gastric mucus, gastric mucosal non-proteic-sulfhydryl groups as well as the increase in thiobarbituric acid-reactive species. Moreover, the gastroprotective effect of BB was effectively blocked in mice pretreated with TRPV1 antagonist capsazepine, by the non-selective cyclooxygenase inhibitor indomethacin, or by the nitric oxide synthase inhibitor L-NAME but not by K(+)(ATP) channel inhibitor glibenclamide. In contrast, the gastroprotective effect of BBOH was blocked only by indomethacin and glibenclamide pretreatments. CONCLUSION: The protective role for BBOH and BB affording gastroprotection against gastric damage induced by ethanol indicates that these compounds contribute for the activity of Plectranthus species. The different modes of action are probably related to differences in their chemical structure. SN - 1872-7573 UR - https://www.unboundmedicine.com/medline/citation/20025953/Gastroprotective_effect_of_barbatusin_and_3_beta_hydroxy_3_deoxibarbatusin_quinonoid_diterpenes_isolated_from_Plectranthus_grandis_in_ethanol_induced_gastric_lesions_in_mice_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0378-8741(09)00739-9 DB - PRIME DP - Unbound Medicine ER -