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Xylans from Scinaia hatei: Structural features, sulfation and anti-HSV activity.
Int J Biol Macromol. 2010 Mar 01; 46(2):173-8.IJ

Abstract

Natural compounds offer interesting pharmacological perspectives for antiviral drug development with regard to broad-spectrum antiviral properties and novel modes of action. In this study, we have analyzed alkali-extracted xylan of Scinaia hatei. Alkali extraction of this red alga yielded a xylan shown to have a molecular mass of 120kDa and a linear structure of (1-->4)-linked beta-d-xylopyranosyl residues. Derivatives (S1, S2, S3 and S4) generated by chemical sulfation from this macromolecule had degree of sulfation between 0.93 and 1.95, and contained strong anti-HSV activity with inhibitory concentration 50% (IC(50)) from 0.22 to 1.37mug/ml. Furthermore, they had no direct inactivating effect on virions in a virucidal assay. Sulfate groups account for their in vitro antiviral activity. Interestingly, sulfated xylans already exerted anti-HSV activity when only pre-incubated with the cultured cells prior to infection, thus pointing to a main inhibitory effect on viral entry.

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Authors+Show Affiliations

Mandal P
Natural Products Laboratory, Department of Chemistry, The University of Burdwan, Golapbag, Burdwan, WB 713104, India.
Pujol CA
No affiliation info available
Damonte EB
No affiliation info available
Ghosh T
No affiliation info available
Ray B
No affiliation info available

MeSH

AnimalsAntiviral AgentsChlorocebus aethiopsChromatography, GelGlycosidesHerpesvirus 1, HumanHerpesvirus 2, HumanMagnetic Resonance SpectroscopyMolecular WeightRhodophytaSulfurVero CellsXylans

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20026104

Citation

Mandal, Pinaki, et al. "Xylans From Scinaia Hatei: Structural Features, Sulfation and anti-HSV Activity." International Journal of Biological Macromolecules, vol. 46, no. 2, 2010, pp. 173-8.
Mandal P, Pujol CA, Damonte EB, et al. Xylans from Scinaia hatei: Structural features, sulfation and anti-HSV activity. Int J Biol Macromol. 2010;46(2):173-8.
Mandal, P., Pujol, C. A., Damonte, E. B., Ghosh, T., & Ray, B. (2010). Xylans from Scinaia hatei: Structural features, sulfation and anti-HSV activity. International Journal of Biological Macromolecules, 46(2), 173-8. https://doi.org/10.1016/j.ijbiomac.2009.12.003
Mandal P, et al. Xylans From Scinaia Hatei: Structural Features, Sulfation and anti-HSV Activity. Int J Biol Macromol. 2010 Mar 1;46(2):173-8. PubMed PMID: 20026104.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Xylans from Scinaia hatei: Structural features, sulfation and anti-HSV activity. AU - Mandal,Pinaki, AU - Pujol,Carlos Alberto, AU - Damonte,Elsa Beatriz, AU - Ghosh,Tuhin, AU - Ray,Bimalendu, Y1 - 2009/12/21/ PY - 2009/08/28/received PY - 2009/11/25/revised PY - 2009/12/09/accepted PY - 2009/12/23/entrez PY - 2009/12/23/pubmed PY - 2010/5/14/medline SP - 173 EP - 8 JF - International journal of biological macromolecules JO - Int J Biol Macromol VL - 46 IS - 2 N2 - Natural compounds offer interesting pharmacological perspectives for antiviral drug development with regard to broad-spectrum antiviral properties and novel modes of action. In this study, we have analyzed alkali-extracted xylan of Scinaia hatei. Alkali extraction of this red alga yielded a xylan shown to have a molecular mass of 120kDa and a linear structure of (1-->4)-linked beta-d-xylopyranosyl residues. Derivatives (S1, S2, S3 and S4) generated by chemical sulfation from this macromolecule had degree of sulfation between 0.93 and 1.95, and contained strong anti-HSV activity with inhibitory concentration 50% (IC(50)) from 0.22 to 1.37mug/ml. Furthermore, they had no direct inactivating effect on virions in a virucidal assay. Sulfate groups account for their in vitro antiviral activity. Interestingly, sulfated xylans already exerted anti-HSV activity when only pre-incubated with the cultured cells prior to infection, thus pointing to a main inhibitory effect on viral entry. SN - 1879-0003 UR - https://www.unboundmedicine.com/medline/citation/20026104/Xylans_from_Scinaia_hatei:_Structural_features_sulfation_and_anti_HSV_activity_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0141-8130(09)00266-9 DB - PRIME DP - Unbound Medicine ER -