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A free-choice high-fat high-sugar diet induces changes in arcuate neuropeptide expression that support hyperphagia.
Int J Obes (Lond). 2010 Mar; 34(3):537-46.IJ

Abstract

OBJECTIVES

The mechanisms for how saturated fat and sugar-based beverages contribute to human obesity are poorly understood. This paper describes a series of experiments developed to examine the response of hypothalamic neuropeptides to diets rich in sugar and fat, using three different diets: a high-fat high-sugar (HFHS) choice diet with access to chow, saturated fat and a 30% sugar solution; a high-fat (HF) choice diet with access to chow and saturated fat; or to a high-sugar (HS) choice diet with access to chow and a sugar solution.

METHOD

We first studied caloric intake, body weight gain, hormonal alterations and hypothalamic neuropeptide expression when male Wistar rats were subjected to an HFHS choice, an HF choice or an HS choice diet for 1 week. Next, we studied caloric intake and body weight gain when rats were subjected to the choice diets for 5 weeks. Finally, we measured neuropeptide expression in hepatic vagotomized rats subjected to an HFHS choice, an HF choice or an HS choice diet for 1 week.

RESULTS

In rats on an HF choice diet, plasma leptin concentrations and proopiomelanocortin (POMC) mRNA increased and neuropeptide Y (NPY) mRNA decreased. Rats on an HFHS choice diet showed identical plasma leptin concentrations as rats on an HF choice diet. However, NPY mRNA increased and POMC mRNA decreased. An HS choice diet for 1 week did not alter hypothalamic neuropeptide expression or plasma leptin concentrations. As hormonal changes did not explain the differences in hypothalamic neuropeptide expression between rats on the choice diets, we addressed whether neuronal feedback signals mediated the hypothalamic neuropeptide response. The POMC mRNA response to different diets depended on an intact innervation of liver and upper intestinal tract.

CONCLUSION

Our data suggest that the specific combination of saturated fat and a 30% sugar solution results in hyperphagia-induced obesity and alters hypothalamic neuropeptide expression, and that the response of the melanocortin system is mediated by the hepatic vagus.

Authors+Show Affiliations

Rudolf Magnus Institute of Neuroscience, Department of Neuroscience and Pharmacology, University Medical Center Utrecht, Utrecht, The Netherlands. s.e.lafleur@amc.uva.nlNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20029382

Citation

la Fleur, S E., et al. "A Free-choice High-fat High-sugar Diet Induces Changes in Arcuate Neuropeptide Expression That Support Hyperphagia." International Journal of Obesity (2005), vol. 34, no. 3, 2010, pp. 537-46.
la Fleur SE, van Rozen AJ, Luijendijk MC, et al. A free-choice high-fat high-sugar diet induces changes in arcuate neuropeptide expression that support hyperphagia. Int J Obes (Lond). 2010;34(3):537-46.
la Fleur, S. E., van Rozen, A. J., Luijendijk, M. C., Groeneweg, F., & Adan, R. A. (2010). A free-choice high-fat high-sugar diet induces changes in arcuate neuropeptide expression that support hyperphagia. International Journal of Obesity (2005), 34(3), 537-46. https://doi.org/10.1038/ijo.2009.257
la Fleur SE, et al. A Free-choice High-fat High-sugar Diet Induces Changes in Arcuate Neuropeptide Expression That Support Hyperphagia. Int J Obes (Lond). 2010;34(3):537-46. PubMed PMID: 20029382.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A free-choice high-fat high-sugar diet induces changes in arcuate neuropeptide expression that support hyperphagia. AU - la Fleur,S E, AU - van Rozen,A J, AU - Luijendijk,M C M, AU - Groeneweg,F, AU - Adan,R A H, Y1 - 2009/12/22/ PY - 2009/12/24/entrez PY - 2009/12/24/pubmed PY - 2010/11/4/medline SP - 537 EP - 46 JF - International journal of obesity (2005) JO - Int J Obes (Lond) VL - 34 IS - 3 N2 - OBJECTIVES: The mechanisms for how saturated fat and sugar-based beverages contribute to human obesity are poorly understood. This paper describes a series of experiments developed to examine the response of hypothalamic neuropeptides to diets rich in sugar and fat, using three different diets: a high-fat high-sugar (HFHS) choice diet with access to chow, saturated fat and a 30% sugar solution; a high-fat (HF) choice diet with access to chow and saturated fat; or to a high-sugar (HS) choice diet with access to chow and a sugar solution. METHOD: We first studied caloric intake, body weight gain, hormonal alterations and hypothalamic neuropeptide expression when male Wistar rats were subjected to an HFHS choice, an HF choice or an HS choice diet for 1 week. Next, we studied caloric intake and body weight gain when rats were subjected to the choice diets for 5 weeks. Finally, we measured neuropeptide expression in hepatic vagotomized rats subjected to an HFHS choice, an HF choice or an HS choice diet for 1 week. RESULTS: In rats on an HF choice diet, plasma leptin concentrations and proopiomelanocortin (POMC) mRNA increased and neuropeptide Y (NPY) mRNA decreased. Rats on an HFHS choice diet showed identical plasma leptin concentrations as rats on an HF choice diet. However, NPY mRNA increased and POMC mRNA decreased. An HS choice diet for 1 week did not alter hypothalamic neuropeptide expression or plasma leptin concentrations. As hormonal changes did not explain the differences in hypothalamic neuropeptide expression between rats on the choice diets, we addressed whether neuronal feedback signals mediated the hypothalamic neuropeptide response. The POMC mRNA response to different diets depended on an intact innervation of liver and upper intestinal tract. CONCLUSION: Our data suggest that the specific combination of saturated fat and a 30% sugar solution results in hyperphagia-induced obesity and alters hypothalamic neuropeptide expression, and that the response of the melanocortin system is mediated by the hepatic vagus. SN - 1476-5497 UR - https://www.unboundmedicine.com/medline/citation/20029382/A_free_choice_high_fat_high_sugar_diet_induces_changes_in_arcuate_neuropeptide_expression_that_support_hyperphagia_ L2 - http://dx.doi.org/10.1038/ijo.2009.257 DB - PRIME DP - Unbound Medicine ER -