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Identification, quantitation, and method validation for flavan-3-ols in fermented ready-to-drink teas from the Italian market using HPLC-UV/DAD and LC-MS/MS.
J Sep Sci. 2009 Nov; 32(21):3643-51.JS

Abstract

The present study is focused on flavan-3-ols characterizing the antioxidant properties of fermented tea (Camellia sinensis). These bioactive compounds, object of nutritional claims in commercial products, should be quantified with rigorous analytical procedures whose accuracy and precision have been stated with a certain level of confidence. An HPLC-UV/DAD method, able to detect and quantify flavan-3-ols in infusions and ready-to-drink teas, has been developed for routine analysis and validated by characterizing several performance parameters. The accuracy assessment has been run through a series of LC-MS/MS analyses. Epigallocatechin, (+)-catechin, (-)-epigallocatechingallate, (-)-epicatechin, (-)-gallocatechingallate, (-)-epicatechingallate, and (-)-catechingallate were chosen as markers of the polyphenolic fraction. Quantitative results showed that samples obtained from tea leaves infusion were richer in polyphenolic antioxidants than those obtained through other industrial processes. The influence of shelf-life and packaging material on the flavan-3-ols content was also considered; markers decreased, with an exponential trend, as a function of time within the shelf life while packaging materials demonstrated to influence differently the flavan-3-ol fraction composition over time. The method presented here provides quantitative results with a certain level of confidence and is suitable for a routine quality control of iced teas whose antioxidant properties are object of nutritional claim.

Authors+Show Affiliations

Dipartimento di Scienza e Tecnologia del Farmaco, Università degli Studi di Torino, Torino, Italy. chiara.cordero@unito.itNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Validation Study

Language

eng

PubMed ID

20029906

Citation

Cordero, Chiara, et al. "Identification, Quantitation, and Method Validation for Flavan-3-ols in Fermented Ready-to-drink Teas From the Italian Market Using HPLC-UV/DAD and LC-MS/MS." Journal of Separation Science, vol. 32, no. 21, 2009, pp. 3643-51.
Cordero C, Canale F, Del Rio D, et al. Identification, quantitation, and method validation for flavan-3-ols in fermented ready-to-drink teas from the Italian market using HPLC-UV/DAD and LC-MS/MS. J Sep Sci. 2009;32(21):3643-51.
Cordero, C., Canale, F., Del Rio, D., & Bicchi, C. (2009). Identification, quantitation, and method validation for flavan-3-ols in fermented ready-to-drink teas from the Italian market using HPLC-UV/DAD and LC-MS/MS. Journal of Separation Science, 32(21), 3643-51. https://doi.org/10.1002/jssc.200900369
Cordero C, et al. Identification, Quantitation, and Method Validation for Flavan-3-ols in Fermented Ready-to-drink Teas From the Italian Market Using HPLC-UV/DAD and LC-MS/MS. J Sep Sci. 2009;32(21):3643-51. PubMed PMID: 20029906.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Identification, quantitation, and method validation for flavan-3-ols in fermented ready-to-drink teas from the Italian market using HPLC-UV/DAD and LC-MS/MS. AU - Cordero,Chiara, AU - Canale,Francesca, AU - Del Rio,Daniele, AU - Bicchi,Carlo, PY - 2009/12/24/entrez PY - 2009/12/24/pubmed PY - 2010/3/2/medline SP - 3643 EP - 51 JF - Journal of separation science JO - J Sep Sci VL - 32 IS - 21 N2 - The present study is focused on flavan-3-ols characterizing the antioxidant properties of fermented tea (Camellia sinensis). These bioactive compounds, object of nutritional claims in commercial products, should be quantified with rigorous analytical procedures whose accuracy and precision have been stated with a certain level of confidence. An HPLC-UV/DAD method, able to detect and quantify flavan-3-ols in infusions and ready-to-drink teas, has been developed for routine analysis and validated by characterizing several performance parameters. The accuracy assessment has been run through a series of LC-MS/MS analyses. Epigallocatechin, (+)-catechin, (-)-epigallocatechingallate, (-)-epicatechin, (-)-gallocatechingallate, (-)-epicatechingallate, and (-)-catechingallate were chosen as markers of the polyphenolic fraction. Quantitative results showed that samples obtained from tea leaves infusion were richer in polyphenolic antioxidants than those obtained through other industrial processes. The influence of shelf-life and packaging material on the flavan-3-ols content was also considered; markers decreased, with an exponential trend, as a function of time within the shelf life while packaging materials demonstrated to influence differently the flavan-3-ol fraction composition over time. The method presented here provides quantitative results with a certain level of confidence and is suitable for a routine quality control of iced teas whose antioxidant properties are object of nutritional claim. SN - 1615-9314 UR - https://www.unboundmedicine.com/medline/citation/20029906/Identification_quantitation_and_method_validation_for_flavan_3_ols_in_fermented_ready_to_drink_teas_from_the_Italian_market_using_HPLC_UV/DAD_and_LC_MS/MS_ L2 - https://doi.org/10.1002/jssc.200900369 DB - PRIME DP - Unbound Medicine ER -