Headspace solid-phase microextraction for direct determination of volatile phenols in cider.
J Sep Sci. 2009 Nov; 32(21):3746-54.JS

Abstract

A headspace solid-phase microextraction coupled to gas chromatography-tandem mass spectrometry (GC-MS/MS) method was optimised and validated for the determination of 4-ethylguaiacol, 4-ethylphenol, 4-vinylguaiacol and 4-vinylphenol, involved in the presence of Brett character, in ciders. The influence of different parameters on extraction efficiency (fibre coating, salt addition, exposure time, extraction temperature and sample volume/total volume ratio) was evaluated. Divinylbenzene/carboxen/PDMS was selected as extraction fibre and the other optimised parameters were as follows: 10 mL of cider, temperature 70 degrees C, extraction time 60 min and addition of 0.4 g/mL of NaCl. The proposed method showed satisfactory linearity. The detection limits obtained were 0.01 microg/L for 4-ethylguaiacol, 0.02 microg/L for 4-ethylphenol, 0.08 microg/L for 4-vinylguaiacol and 0.03 microg/L for 4-vinylphenol. These detection limits were lower than those obtained in previous studies on the determination of volatile phenols in other alcoholic beverages. Good recoveries of over 95% were observed for all compounds, and the repeatability obtained was considered acceptable, ranging between 4 and 10%. To demonstrate the feasibility of the procedure, the method was applied to the analysis of commercial ciders. To our knowledge, this is the first time that the headspace solid-phase microextraction procedure has been optimised to determine specifically the Brett character responsible compounds in cider.

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Authors+Show Affiliations

Pizarro C

Department of Chemistry, University of La Rioja, Logroño, La Rioja, Spain. consuelo.pizarro@unirioja.es

Pérez-del-Notario N

No affiliation info available

González-Sáiz JM

No affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

20029909

Citation

Pizarro, Consuelo, et al. "Headspace Solid-phase Microextraction for Direct Determination of Volatile Phenols in Cider." Journal of Separation Science, vol. 32, no. 21, 2009, pp. 3746-54.

Pizarro C, Pérez-del-Notario N, González-Sáiz JM. Headspace solid-phase microextraction for direct determination of volatile phenols in cider. J Sep Sci. 2009;32(21):3746-54.

Pizarro, C., Pérez-del-Notario, N., & González-Sáiz, J. M. (2009). Headspace solid-phase microextraction for direct determination of volatile phenols in cider. Journal of Separation Science, 32(21), 3746-54. https://doi.org/10.1002/jssc.200900347

Pizarro C, Pérez-del-Notario N, González-Sáiz JM. Headspace Solid-phase Microextraction for Direct Determination of Volatile Phenols in Cider. J Sep Sci. 2009;32(21):3746-54. PubMed PMID: 20029909.

* Article titles in AMA citation format should be in sentence-case

TY - JOUR T1 - Headspace solid-phase microextraction for direct determination of volatile phenols in cider. AU - Pizarro,Consuelo, AU - Pérez-del-Notario,Nuria, AU - González-Sáiz,José María, PY - 2009/12/24/entrez PY - 2009/12/24/pubmed PY - 2009/12/24/medline SP - 3746 EP - 54 JF - Journal of separation science JO - J Sep Sci VL - 32 IS - 21 N2 - A headspace solid-phase microextraction coupled to gas chromatography-tandem mass spectrometry (GC-MS/MS) method was optimised and validated for the determination of 4-ethylguaiacol, 4-ethylphenol, 4-vinylguaiacol and 4-vinylphenol, involved in the presence of Brett character, in ciders. The influence of different parameters on extraction efficiency (fibre coating, salt addition, exposure time, extraction temperature and sample volume/total volume ratio) was evaluated. Divinylbenzene/carboxen/PDMS was selected as extraction fibre and the other optimised parameters were as follows: 10 mL of cider, temperature 70 degrees C, extraction time 60 min and addition of 0.4 g/mL of NaCl. The proposed method showed satisfactory linearity. The detection limits obtained were 0.01 microg/L for 4-ethylguaiacol, 0.02 microg/L for 4-ethylphenol, 0.08 microg/L for 4-vinylguaiacol and 0.03 microg/L for 4-vinylphenol. These detection limits were lower than those obtained in previous studies on the determination of volatile phenols in other alcoholic beverages. Good recoveries of over 95% were observed for all compounds, and the repeatability obtained was considered acceptable, ranging between 4 and 10%. To demonstrate the feasibility of the procedure, the method was applied to the analysis of commercial ciders. To our knowledge, this is the first time that the headspace solid-phase microextraction procedure has been optimised to determine specifically the Brett character responsible compounds in cider. SN - 1615-9314 UR - https://www.unboundmedicine.com/medline/citation/20029909/Headspace_solid_phase_microextraction_for_direct_determination_of_volatile_phenols_in_cider_ L2 - https://doi.org/10.1002/jssc.200900347 DB - PRIME DP - Unbound Medicine ER -

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Headspace solid-phase microextraction for direct determination of volatile phenols in cider.J Sep Sci. 2009 Nov; 32(21):3746-54.JS