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[Chromatographic fingerprints of triterpenoid constituents of Ganoderma lucidum].
Zhongguo Zhong Yao Za Zhi. 2009 Sep; 34(18):2356-9.ZZ

Abstract

OBJECTIVE

To establish chromatographic fingerprints of triterpenoid compounds in ganoderma RP-HPLC for the quality control of ganoderma.

METHOD

HPLC fingerprints were established. The chromatographic conditions were as follows: the separation was performed on a Diamonsil C18 column (4.6 mm x 250 mm, 5 microm), using a gradient elution with acetonitrile-water containing 0.8% perchloric acid as the mobile phases. The flow rate was 0.9 mL x min(-1), the detection wavelength was 254 nm and the temperature of column was at ambient temperature. The common patterns of HPTLC and HPLC fingerprints were separately obtained through "Chromafinger" solution software, and quality assessments were analyzed by similarity.

RESULT

The HPLC fingerprint pattern of G. lucidum consists of 18 peaks, among them 6 peaks were identified by chemical reference substances.

CONCLUSION

This study provided theory and experimental data in order to comprehensively evaluate the quality of ganoderma.

Authors+Show Affiliations

Ding P
College of Chinese Materia Medica, Guangzhou University of Chinese Madicine, Guangzhou 510405, China. dingpinggz@126.com
Qiu J
No affiliation info available
Liang Y
No affiliation info available
Wang H
No affiliation info available

MeSH

Chromatography, High Pressure LiquidDrugs, Chinese HerbalReishiTriterpenes

Pub Type(s)

Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't

Language

chi

PubMed ID

20030088

Citation

Ding, Ping, et al. "[Chromatographic Fingerprints of Triterpenoid Constituents of Ganoderma Lucidum]." Zhongguo Zhong Yao Za Zhi = Zhongguo Zhongyao Zazhi = China Journal of Chinese Materia Medica, vol. 34, no. 18, 2009, pp. 2356-9.
Ding P, Qiu J, Liang Y, et al. [Chromatographic fingerprints of triterpenoid constituents of Ganoderma lucidum]. Zhongguo Zhong Yao Za Zhi. 2009;34(18):2356-9.
Ding, P., Qiu, J., Liang, Y., & Wang, H. (2009). [Chromatographic fingerprints of triterpenoid constituents of Ganoderma lucidum]. Zhongguo Zhong Yao Za Zhi = Zhongguo Zhongyao Zazhi = China Journal of Chinese Materia Medica, 34(18), 2356-9.
Ding P, et al. [Chromatographic Fingerprints of Triterpenoid Constituents of Ganoderma Lucidum]. Zhongguo Zhong Yao Za Zhi. 2009;34(18):2356-9. PubMed PMID: 20030088.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Chromatographic fingerprints of triterpenoid constituents of Ganoderma lucidum]. AU - Ding,Ping, AU - Qiu,Jinying, AU - Liang,Yingjiao, AU - Wang,Huiling, PY - 2009/12/25/entrez PY - 2009/12/25/pubmed PY - 2010/1/13/medline SP - 2356 EP - 9 JF - Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica JO - Zhongguo Zhong Yao Za Zhi VL - 34 IS - 18 N2 - OBJECTIVE: To establish chromatographic fingerprints of triterpenoid compounds in ganoderma RP-HPLC for the quality control of ganoderma. METHOD: HPLC fingerprints were established. The chromatographic conditions were as follows: the separation was performed on a Diamonsil C18 column (4.6 mm x 250 mm, 5 microm), using a gradient elution with acetonitrile-water containing 0.8% perchloric acid as the mobile phases. The flow rate was 0.9 mL x min(-1), the detection wavelength was 254 nm and the temperature of column was at ambient temperature. The common patterns of HPTLC and HPLC fingerprints were separately obtained through "Chromafinger" solution software, and quality assessments were analyzed by similarity. RESULT: The HPLC fingerprint pattern of G. lucidum consists of 18 peaks, among them 6 peaks were identified by chemical reference substances. CONCLUSION: This study provided theory and experimental data in order to comprehensively evaluate the quality of ganoderma. SN - 1001-5302 UR - https://www.unboundmedicine.com/medline/citation/20030088/[Chromatographic_fingerprints_of_triterpenoid_constituents_of_Ganoderma_lucidum]_ DB - PRIME DP - Unbound Medicine ER -