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Comparative evaluation of five culture media with triplex PCR assay for detection of methicillin-resistant Staphylococcus aureus.
Curr Microbiol. 2010 Jul; 61(1):1-6.CM

Abstract

The emergence of methicillin-resistant Staphylococcus aureus (MRSA) is responsible for nosocomial and community-acquired infections. Hence, rapid and accurate laboratory diagnosis of MRSA is a vital constituent of control measures. The present study evaluated five different methods for the identification of MRSA. A total of 207 S. aureus clinical isolates that consisted of 89 MRSA and 118 methicillin-susceptible S. aureus (MSSA) strains confirmed by PCR were tested. MRSA strains were evaluated by five different methods: chromogenic MRSA agar (CMRSA), oxacillin resistance screening agar base (ORSAB), mannitol salt oxacillin agar (MSO), mannitol salt cefoxitin agar with two different concentrations of cefoxitin [4 microg/ml (MSC-4) and 6 microg/ml (MSC-6)]. The results of the different methods were compared to mecA PCR as the gold standard. MSC-6 showed only six false-positive MRSA in comparison with PCR. The sensitivities and specificities of MSC-6, MSC-4, MSO-4, ORSAB, and CMRSA were as follows: 98.9/94.9%, 100/83.1%, 89.9/87.3%, 97.8/96.6%, and 95.5/94.9%, respectively. In comparison with PCR, it was found that both MSC-6 and ORSAB were relatively the least expensive screening tests ($0.70 and $1.00, respectively). In conclusion, all methods were comparable, but MSC-6 was the least expensive medium for MRSA screening.

Authors+Show Affiliations

Department of Medical Microbiology and Parasitology, School of Medical Sciences, Universiti Sains Malaysia, Kubang Kerian, 16150, Kota Bharu, Kelantan, Malaysia. hassanainiy@yahoo.comNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20033170

Citation

Al-Talib, Hassanain, et al. "Comparative Evaluation of Five Culture Media With Triplex PCR Assay for Detection of Methicillin-resistant Staphylococcus Aureus." Current Microbiology, vol. 61, no. 1, 2010, pp. 1-6.
Al-Talib H, Yean CY, Al-khateeb A, et al. Comparative evaluation of five culture media with triplex PCR assay for detection of methicillin-resistant Staphylococcus aureus. Curr Microbiol. 2010;61(1):1-6.
Al-Talib, H., Yean, C. Y., Al-khateeb, A., Singh, K. K., Hasan, H., Al-Jashamy, K., & Ravichandran, M. (2010). Comparative evaluation of five culture media with triplex PCR assay for detection of methicillin-resistant Staphylococcus aureus. Current Microbiology, 61(1), 1-6. https://doi.org/10.1007/s00284-009-9567-8
Al-Talib H, et al. Comparative Evaluation of Five Culture Media With Triplex PCR Assay for Detection of Methicillin-resistant Staphylococcus Aureus. Curr Microbiol. 2010;61(1):1-6. PubMed PMID: 20033170.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Comparative evaluation of five culture media with triplex PCR assay for detection of methicillin-resistant Staphylococcus aureus. AU - Al-Talib,Hassanain, AU - Yean,Chan Yean, AU - Al-khateeb,Alyaa, AU - Singh,Kirnpal-Kaur Banga, AU - Hasan,Habsah, AU - Al-Jashamy,Karim, AU - Ravichandran,Manickam, Y1 - 2009/12/24/ PY - 2009/06/22/received PY - 2009/12/08/accepted PY - 2009/12/25/entrez PY - 2009/12/25/pubmed PY - 2010/7/29/medline SP - 1 EP - 6 JF - Current microbiology JO - Curr. Microbiol. VL - 61 IS - 1 N2 - The emergence of methicillin-resistant Staphylococcus aureus (MRSA) is responsible for nosocomial and community-acquired infections. Hence, rapid and accurate laboratory diagnosis of MRSA is a vital constituent of control measures. The present study evaluated five different methods for the identification of MRSA. A total of 207 S. aureus clinical isolates that consisted of 89 MRSA and 118 methicillin-susceptible S. aureus (MSSA) strains confirmed by PCR were tested. MRSA strains were evaluated by five different methods: chromogenic MRSA agar (CMRSA), oxacillin resistance screening agar base (ORSAB), mannitol salt oxacillin agar (MSO), mannitol salt cefoxitin agar with two different concentrations of cefoxitin [4 microg/ml (MSC-4) and 6 microg/ml (MSC-6)]. The results of the different methods were compared to mecA PCR as the gold standard. MSC-6 showed only six false-positive MRSA in comparison with PCR. The sensitivities and specificities of MSC-6, MSC-4, MSO-4, ORSAB, and CMRSA were as follows: 98.9/94.9%, 100/83.1%, 89.9/87.3%, 97.8/96.6%, and 95.5/94.9%, respectively. In comparison with PCR, it was found that both MSC-6 and ORSAB were relatively the least expensive screening tests ($0.70 and $1.00, respectively). In conclusion, all methods were comparable, but MSC-6 was the least expensive medium for MRSA screening. SN - 1432-0991 UR - https://www.unboundmedicine.com/medline/citation/20033170/Comparative_evaluation_of_five_culture_media_with_triplex_PCR_assay_for_detection_of_methicillin_resistant_Staphylococcus_aureus_ L2 - https://dx.doi.org/10.1007/s00284-009-9567-8 DB - PRIME DP - Unbound Medicine ER -