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Correlation of omega-3 levels in serum phospholipid from 2053 human blood samples with key fatty acid ratios.
Nutr J 2009; 8:58NJ

Abstract

BACKGROUND

This research was conducted to explore the relationships between the levels of omega-3 fatty acids in serum phospholipid and key fatty acid ratios including potential cut-offs for risk factor assessment with respect to coronary heart disease and fatal ischemic heart disease.

METHODS

Blood samples (n = 2053) were obtained from free-living subjects in North America and processed for determining the levels of total fatty acids in serum phospholipid as omega-3 fatty acids including EPA (eicosapentaenoic acid, 20:5 n-3) and DHA (docosahexaenoic acid, 22:6 n-3) by combined thin-layer and gas-liquid chromatographic analyses. The omega-3 levels were correlated with selected omega-6: omega-3 ratios including AA (arachidonic acid, 20:4n-6): EPA and AA:(EPA+DHA). Based on previously-published levels of omega-3 fatty acids considered to be in a 'lower risk' category for heart disease and related fatality, 'lower risk' categories for selected fatty acid ratios were estimated.

RESULTS

Strong inverse correlations between the summed total of omega-3 fatty acids in serum phospholipid and all four ratios (omega-6:omega-3 (n-6:n-3), AA:EPA, AA:DHA, and AA:(EPA+DHA)) were found with the most potent correlation being with the omega-6:omega-3 ratio (R(2) = 0.96). The strongest inverse relation for the EPA+DHA levels in serum phospholipid was found with the omega-6: omega-3 ratio (R(2) = 0.94) followed closely by the AA:(EPA+DHA) ratio at R(2) = 0.88. It was estimated that 95% of the subjects would be in the 'lower risk' category for coronary heart disease (based on total omega-3 >or= 7.2%) with omega-6:omega-3 ratios <4.5 and AA:(EPA+DHA) ratios <1.4. The corresponding ratio cut-offs for a 'lower risk' category for fatal ischemic heart disease (EPA+DHA >or= 4.6%) were estimated at < 5.8 and < 2.1, respectively.

CONCLUSIONS

Strong inverse correlations between the levels of omega-3 fatty acids in serum (or plasma) phospholipid and omega-6: omega-3 ratios are apparent based on this large database of 2053 samples. Certain fatty acid ratios may aid in cardiovascular disease-related risk assessment if/when complete profiles are not available.

Authors+Show Affiliations

Department of Human Health and Nutritional Sciences, University of Guelph, Guelph, Ontario, N1G 2W1, Canada. bholub@uoguelph.caNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

20034401

Citation

Holub, Bruce J., et al. "Correlation of Omega-3 Levels in Serum Phospholipid From 2053 Human Blood Samples With Key Fatty Acid Ratios." Nutrition Journal, vol. 8, 2009, p. 58.
Holub BJ, Wlodek M, Rowe W, et al. Correlation of omega-3 levels in serum phospholipid from 2053 human blood samples with key fatty acid ratios. Nutr J. 2009;8:58.
Holub, B. J., Wlodek, M., Rowe, W., & Piekarski, J. (2009). Correlation of omega-3 levels in serum phospholipid from 2053 human blood samples with key fatty acid ratios. Nutrition Journal, 8, p. 58. doi:10.1186/1475-2891-8-58.
Holub BJ, et al. Correlation of Omega-3 Levels in Serum Phospholipid From 2053 Human Blood Samples With Key Fatty Acid Ratios. Nutr J. 2009 Dec 24;8:58. PubMed PMID: 20034401.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Correlation of omega-3 levels in serum phospholipid from 2053 human blood samples with key fatty acid ratios. AU - Holub,Bruce J, AU - Wlodek,Mike, AU - Rowe,William, AU - Piekarski,Jerry, Y1 - 2009/12/24/ PY - 2008/08/11/received PY - 2009/12/24/accepted PY - 2009/12/26/entrez PY - 2009/12/26/pubmed PY - 2010/2/19/medline SP - 58 EP - 58 JF - Nutrition journal JO - Nutr J VL - 8 N2 - BACKGROUND: This research was conducted to explore the relationships between the levels of omega-3 fatty acids in serum phospholipid and key fatty acid ratios including potential cut-offs for risk factor assessment with respect to coronary heart disease and fatal ischemic heart disease. METHODS: Blood samples (n = 2053) were obtained from free-living subjects in North America and processed for determining the levels of total fatty acids in serum phospholipid as omega-3 fatty acids including EPA (eicosapentaenoic acid, 20:5 n-3) and DHA (docosahexaenoic acid, 22:6 n-3) by combined thin-layer and gas-liquid chromatographic analyses. The omega-3 levels were correlated with selected omega-6: omega-3 ratios including AA (arachidonic acid, 20:4n-6): EPA and AA:(EPA+DHA). Based on previously-published levels of omega-3 fatty acids considered to be in a 'lower risk' category for heart disease and related fatality, 'lower risk' categories for selected fatty acid ratios were estimated. RESULTS: Strong inverse correlations between the summed total of omega-3 fatty acids in serum phospholipid and all four ratios (omega-6:omega-3 (n-6:n-3), AA:EPA, AA:DHA, and AA:(EPA+DHA)) were found with the most potent correlation being with the omega-6:omega-3 ratio (R(2) = 0.96). The strongest inverse relation for the EPA+DHA levels in serum phospholipid was found with the omega-6: omega-3 ratio (R(2) = 0.94) followed closely by the AA:(EPA+DHA) ratio at R(2) = 0.88. It was estimated that 95% of the subjects would be in the 'lower risk' category for coronary heart disease (based on total omega-3 >or= 7.2%) with omega-6:omega-3 ratios <4.5 and AA:(EPA+DHA) ratios <1.4. The corresponding ratio cut-offs for a 'lower risk' category for fatal ischemic heart disease (EPA+DHA >or= 4.6%) were estimated at < 5.8 and < 2.1, respectively. CONCLUSIONS: Strong inverse correlations between the levels of omega-3 fatty acids in serum (or plasma) phospholipid and omega-6: omega-3 ratios are apparent based on this large database of 2053 samples. Certain fatty acid ratios may aid in cardiovascular disease-related risk assessment if/when complete profiles are not available. SN - 1475-2891 UR - https://www.unboundmedicine.com/medline/citation/20034401/Correlation_of_omega_3_levels_in_serum_phospholipid_from_2053_human_blood_samples_with_key_fatty_acid_ratios_ L2 - https://nutritionj.biomedcentral.com/articles/10.1186/1475-2891-8-58 DB - PRIME DP - Unbound Medicine ER -