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Effects of ubiquinone on hydroperoxide concentration and antioxidant enzymatic activities in the rat hippocampus during pilocarpine-induced seizures.
Brain Res. 2010 Feb 22; 1315:33-40.BR

Abstract

Recent researches have shown that antioxidant compounds may have certain neuroprotective effect against the neurotoxicity of seizures at cellular level. Ubiquinone (UQ), an antioxidant compound, exhibits a wide range of therapeutic effects that are attributed to its potent antioxidant capacity. The objective of the present study was to evaluate the neuroprotective effects of UQ in rats, against the observed oxidative stress during seizures induced by pilocarpine. Wistar rats were treated with either 0.9% saline (i.p., control group), UQ (5, 10 or 20 mg/kg, i.p., UQ5, UQ10 and UQ20 groups), pilocarpine (400 mg/kg, i.p., P400 group), or co-administration of pilocarpine with UQ group rats 30 min prior to UQ administration. After the treatments all groups were observed for 24 h. The antioxidant enzymatic activities as well as the hydroperoxide concentrations were measured using spectrophotometric methods and the results were analyzed. In pilocarpine group there was a significant increase in hydroperoxides concentration and glutathione peroxidase activity. However, no alteration was observed in superoxide dismutase and catalase activities. Antioxidant treatment significantly reduced the hydroperoxide content and increased the superoxide dismutase, catalase and glutathione peroxidase activities in rat hippocampus during seizures induced by pilocarpine. Our findings strongly support the hypothesis that oxidative stress in hippocampus occurs during seizures induced by pilocarpine, which indicates that brain damage induced by the oxidative process plays a crucial role in seizures pathogenic consequences. Our result also suggests that ubiquinone can exert significant neuroprotective effects that might be useful in the treatment of neurodegenerative diseases.

Authors+Show Affiliations

Laboratory Experimental Research in Biological Sciences, Federal University of Piaui, Piaui, Brazil.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20034476

Citation

Santos, Itala Mônica de Sales, et al. "Effects of Ubiquinone On Hydroperoxide Concentration and Antioxidant Enzymatic Activities in the Rat Hippocampus During Pilocarpine-induced Seizures." Brain Research, vol. 1315, 2010, pp. 33-40.
Santos IM, de Freitas RL, da Silva EP, et al. Effects of ubiquinone on hydroperoxide concentration and antioxidant enzymatic activities in the rat hippocampus during pilocarpine-induced seizures. Brain Res. 2010;1315:33-40.
Santos, I. M., de Freitas, R. L., da Silva, E. P., Feitosa, C. M., Saldanha, G. B., Souza, G. F., Tomé, A. d. a. . R., Feng, D., & de Freitas, R. M. (2010). Effects of ubiquinone on hydroperoxide concentration and antioxidant enzymatic activities in the rat hippocampus during pilocarpine-induced seizures. Brain Research, 1315, 33-40. https://doi.org/10.1016/j.brainres.2009.12.037
Santos IM, et al. Effects of Ubiquinone On Hydroperoxide Concentration and Antioxidant Enzymatic Activities in the Rat Hippocampus During Pilocarpine-induced Seizures. Brain Res. 2010 Feb 22;1315:33-40. PubMed PMID: 20034476.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effects of ubiquinone on hydroperoxide concentration and antioxidant enzymatic activities in the rat hippocampus during pilocarpine-induced seizures. AU - Santos,Itala Mônica de Sales, AU - de Freitas,Rizângela Lyne Mendes, AU - da Silva,Elisângela Pascoal, AU - Feitosa,Chistiane Mendes, AU - Saldanha,Gláucio Barros, AU - Souza,Geane Felix, AU - Tomé,Adriana da Rocha, AU - Feng,Dejiang, AU - de Freitas,Rivelilson Mendes, Y1 - 2009/12/23/ PY - 2009/10/26/received PY - 2009/12/09/revised PY - 2009/12/12/accepted PY - 2009/12/26/entrez PY - 2009/12/26/pubmed PY - 2010/5/7/medline SP - 33 EP - 40 JF - Brain research JO - Brain Res VL - 1315 N2 - Recent researches have shown that antioxidant compounds may have certain neuroprotective effect against the neurotoxicity of seizures at cellular level. Ubiquinone (UQ), an antioxidant compound, exhibits a wide range of therapeutic effects that are attributed to its potent antioxidant capacity. The objective of the present study was to evaluate the neuroprotective effects of UQ in rats, against the observed oxidative stress during seizures induced by pilocarpine. Wistar rats were treated with either 0.9% saline (i.p., control group), UQ (5, 10 or 20 mg/kg, i.p., UQ5, UQ10 and UQ20 groups), pilocarpine (400 mg/kg, i.p., P400 group), or co-administration of pilocarpine with UQ group rats 30 min prior to UQ administration. After the treatments all groups were observed for 24 h. The antioxidant enzymatic activities as well as the hydroperoxide concentrations were measured using spectrophotometric methods and the results were analyzed. In pilocarpine group there was a significant increase in hydroperoxides concentration and glutathione peroxidase activity. However, no alteration was observed in superoxide dismutase and catalase activities. Antioxidant treatment significantly reduced the hydroperoxide content and increased the superoxide dismutase, catalase and glutathione peroxidase activities in rat hippocampus during seizures induced by pilocarpine. Our findings strongly support the hypothesis that oxidative stress in hippocampus occurs during seizures induced by pilocarpine, which indicates that brain damage induced by the oxidative process plays a crucial role in seizures pathogenic consequences. Our result also suggests that ubiquinone can exert significant neuroprotective effects that might be useful in the treatment of neurodegenerative diseases. SN - 1872-6240 UR - https://www.unboundmedicine.com/medline/citation/20034476/Effects_of_ubiquinone_on_hydroperoxide_concentration_and_antioxidant_enzymatic_activities_in_the_rat_hippocampus_during_pilocarpine_induced_seizures_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0006-8993(09)02706-1 DB - PRIME DP - Unbound Medicine ER -