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Correlation between peripheral blood T-cell profiles and clinical and inflammatory parameters in stable COPD.
Allergol Int. 2010 Mar; 59(1):75-82.AI

Abstract

BACKGROUND

Recent studies suggest that Tc1/Tc2 imbalances are implicated in the pathogenesis of chronic obstructive pulmonary disease (COPD). The purpose of this study was to clarify the relationship between peripheral blood T-cell profiles and pulmonary function or inflammatory parameters.

METHODS

Thirty-one patients with stable COPD (median age 70 years, 30 males, 15 current smokers and 16 ex-smokers) and 30 healthy control subjects were enrolled in this study. The subjects underwent blood tests, exhaled nitric oxide (eNO) measurement, pulmonary function tests, and sputum induction. Tc1/Tc2 and Th1/Th2 were determined by analyzing intracellular cytokine staining for IFN-gamma and IL-4 in peripheral blood CD8+ and CD4+ T cells using flow cytometry after stimulation with phorbol 12-myristate 13-acetate and ionomycin.

RESULTS

There was a significantly increased proportion of IFN-gamma-producing and IL-4-producing CD8+ T cells in patients with COPD compared with control subjects (median [IQR] 73.6% [63.9%-80.7%] vs 62.0% [45.6%-73.8%], p=0.004; and 2.6% [1.1%-6.9%] vs 1.1% [0.6%-2.2%], p=0.002, respectively). In addition, the proportion of IFN-gamma-producing CD4+ T cells was significantly higher in patients with COPD compared with control subjects (25.7% [21.2%-38.0%] vs 22.8% [15.6%-29.2%], p=0.027). The proportion of IFN-gamma-producing CD8+ T cells was correlated negatively with single-breath carbon monoxide transfer coefficient (Kco)(rho=-0.45, p=0.033) and positively with eNO (rho=0.50, p=0.012). The proportion of IL-4-producing CD8+ T cells was positively correlated with body mass index (rho=0.42, p=0.023) and Kco (rho=0.47, p=0.026).

CONCLUSIONS

It is suggested that Tc1 cells have a detrimental role and that Tc2 cells have a protective role in disease progression.

Authors+Show Affiliations

Department of Respiratory Medicine, Shizuoka General Hospital, and Hamamatsu University School of Medicine, Shizuoka, Japan. tmjkshi@general-hosp.pref.shizuoka.jpNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

20035145

Citation

Shirai, Toshihiro, et al. "Correlation Between Peripheral Blood T-cell Profiles and Clinical and Inflammatory Parameters in Stable COPD." Allergology International : Official Journal of the Japanese Society of Allergology, vol. 59, no. 1, 2010, pp. 75-82.
Shirai T, Suda T, Inui N, et al. Correlation between peripheral blood T-cell profiles and clinical and inflammatory parameters in stable COPD. Allergol Int. 2010;59(1):75-82.
Shirai, T., Suda, T., Inui, N., & Chida, K. (2010). Correlation between peripheral blood T-cell profiles and clinical and inflammatory parameters in stable COPD. Allergology International : Official Journal of the Japanese Society of Allergology, 59(1), 75-82. https://doi.org/10.2332/allergolint.09-OA-0126
Shirai T, et al. Correlation Between Peripheral Blood T-cell Profiles and Clinical and Inflammatory Parameters in Stable COPD. Allergol Int. 2010;59(1):75-82. PubMed PMID: 20035145.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Correlation between peripheral blood T-cell profiles and clinical and inflammatory parameters in stable COPD. AU - Shirai,Toshihiro, AU - Suda,Takafumi, AU - Inui,Naoki, AU - Chida,Kingo, Y1 - 2009/12/25/ PY - 2009/06/01/received PY - 2009/07/31/accepted PY - 2009/12/26/entrez PY - 2009/12/26/pubmed PY - 2010/6/15/medline SP - 75 EP - 82 JF - Allergology international : official journal of the Japanese Society of Allergology JO - Allergol Int VL - 59 IS - 1 N2 - BACKGROUND: Recent studies suggest that Tc1/Tc2 imbalances are implicated in the pathogenesis of chronic obstructive pulmonary disease (COPD). The purpose of this study was to clarify the relationship between peripheral blood T-cell profiles and pulmonary function or inflammatory parameters. METHODS: Thirty-one patients with stable COPD (median age 70 years, 30 males, 15 current smokers and 16 ex-smokers) and 30 healthy control subjects were enrolled in this study. The subjects underwent blood tests, exhaled nitric oxide (eNO) measurement, pulmonary function tests, and sputum induction. Tc1/Tc2 and Th1/Th2 were determined by analyzing intracellular cytokine staining for IFN-gamma and IL-4 in peripheral blood CD8+ and CD4+ T cells using flow cytometry after stimulation with phorbol 12-myristate 13-acetate and ionomycin. RESULTS: There was a significantly increased proportion of IFN-gamma-producing and IL-4-producing CD8+ T cells in patients with COPD compared with control subjects (median [IQR] 73.6% [63.9%-80.7%] vs 62.0% [45.6%-73.8%], p=0.004; and 2.6% [1.1%-6.9%] vs 1.1% [0.6%-2.2%], p=0.002, respectively). In addition, the proportion of IFN-gamma-producing CD4+ T cells was significantly higher in patients with COPD compared with control subjects (25.7% [21.2%-38.0%] vs 22.8% [15.6%-29.2%], p=0.027). The proportion of IFN-gamma-producing CD8+ T cells was correlated negatively with single-breath carbon monoxide transfer coefficient (Kco)(rho=-0.45, p=0.033) and positively with eNO (rho=0.50, p=0.012). The proportion of IL-4-producing CD8+ T cells was positively correlated with body mass index (rho=0.42, p=0.023) and Kco (rho=0.47, p=0.026). CONCLUSIONS: It is suggested that Tc1 cells have a detrimental role and that Tc2 cells have a protective role in disease progression. SN - 1440-1592 UR - https://www.unboundmedicine.com/medline/citation/20035145/Correlation_between_peripheral_blood_T_cell_profiles_and_clinical_and_inflammatory_parameters_in_stable_COPD_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1323-8930(15)30578-5 DB - PRIME DP - Unbound Medicine ER -