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Deleterious effects of plant cystatins against the banana weevil Cosmopolites sordidus.
Arch Insect Biochem Physiol. 2010 Feb; 73(2):87-105.AI

Abstract

The general potential of plant cystatins for the development of insect-resistant transgenic plants still remains to be established given the natural ability of several insects to compensate for the loss of digestive cysteine protease activities. Here we assessed the potential of cystatins for the development of banana lines resistant to the banana weevil Cosmopolites sordidus, a major pest of banana and plantain in Africa. Protease inhibitory assays were conducted with protein and methylcoumarin (MCA) peptide substrates to measure the inhibitory efficiency of different cystatins in vitro, followed by a diet assay with cystatin-infiltrated banana stem disks to monitor the impact of two plant cystatins, oryzacystatin I (OC-I, or OsCYS1) and papaya cystatin (CpCYS1), on the overall growth rate of weevil larvae. As observed earlier for other Coleoptera, banana weevils produce a variety of proteases for dietary protein digestion, including in particular Z-Phe-Arg-MCA-hydrolyzing (cathepsin L-like) and Z-Arg-Arg-MCA-hydrolyzing (cathepsin B-like) proteases active in mildly acidic conditions. Both enzyme populations were sensitive to the cysteine protease inhibitor E-64 and to different plant cystatins including OsCYS1. In line with the broad inhibitory effects of cystatins, OsCYS1 and CpCYS1 caused an important growth delay in young larvae developing for 10 days in cystatin-infiltrated banana stem disks. These promising results, which illustrate the susceptibility of C. sordidus to plant cystatins, are discussed in the light of recent hypotheses suggesting a key role for cathepsin B-like enzymes as a determinant for resistance or susceptibility to plant cystatins in Coleoptera.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Kiggundu A
Kawanda Agricultural Research Institute, Kampala, Uganda.
Muchwezi J
No affiliation info available
Van der Vyver C
No affiliation info available
Viljoen A
No affiliation info available
Vorster J
No affiliation info available
Schlüter U
No affiliation info available
Kunert K
No affiliation info available
Michaud D
No affiliation info available

MeSH

AnimalsCathepsin BCathepsin LCystatinsCysteine Proteinase InhibitorsInsect ProteinsLarvaMusaPlant ProteinsWeevils

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20035549

Citation

Kiggundu, Andrew, et al. "Deleterious Effects of Plant Cystatins Against the Banana Weevil Cosmopolites Sordidus." Archives of Insect Biochemistry and Physiology, vol. 73, no. 2, 2010, pp. 87-105.
Kiggundu A, Muchwezi J, Van der Vyver C, et al. Deleterious effects of plant cystatins against the banana weevil Cosmopolites sordidus. Arch Insect Biochem Physiol. 2010;73(2):87-105.
Kiggundu, A., Muchwezi, J., Van der Vyver, C., Viljoen, A., Vorster, J., Schlüter, U., Kunert, K., & Michaud, D. (2010). Deleterious effects of plant cystatins against the banana weevil Cosmopolites sordidus. Archives of Insect Biochemistry and Physiology, 73(2), 87-105. https://doi.org/10.1002/arch.20342
Kiggundu A, et al. Deleterious Effects of Plant Cystatins Against the Banana Weevil Cosmopolites Sordidus. Arch Insect Biochem Physiol. 2010;73(2):87-105. PubMed PMID: 20035549.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Deleterious effects of plant cystatins against the banana weevil Cosmopolites sordidus. AU - Kiggundu,Andrew, AU - Muchwezi,Josephine, AU - Van der Vyver,Christell, AU - Viljoen,Altus, AU - Vorster,Juan, AU - Schlüter,Urte, AU - Kunert,Karl, AU - Michaud,Dominique, PY - 2009/12/26/entrez PY - 2009/12/26/pubmed PY - 2010/4/7/medline SP - 87 EP - 105 JF - Archives of insect biochemistry and physiology JO - Arch Insect Biochem Physiol VL - 73 IS - 2 N2 - The general potential of plant cystatins for the development of insect-resistant transgenic plants still remains to be established given the natural ability of several insects to compensate for the loss of digestive cysteine protease activities. Here we assessed the potential of cystatins for the development of banana lines resistant to the banana weevil Cosmopolites sordidus, a major pest of banana and plantain in Africa. Protease inhibitory assays were conducted with protein and methylcoumarin (MCA) peptide substrates to measure the inhibitory efficiency of different cystatins in vitro, followed by a diet assay with cystatin-infiltrated banana stem disks to monitor the impact of two plant cystatins, oryzacystatin I (OC-I, or OsCYS1) and papaya cystatin (CpCYS1), on the overall growth rate of weevil larvae. As observed earlier for other Coleoptera, banana weevils produce a variety of proteases for dietary protein digestion, including in particular Z-Phe-Arg-MCA-hydrolyzing (cathepsin L-like) and Z-Arg-Arg-MCA-hydrolyzing (cathepsin B-like) proteases active in mildly acidic conditions. Both enzyme populations were sensitive to the cysteine protease inhibitor E-64 and to different plant cystatins including OsCYS1. In line with the broad inhibitory effects of cystatins, OsCYS1 and CpCYS1 caused an important growth delay in young larvae developing for 10 days in cystatin-infiltrated banana stem disks. These promising results, which illustrate the susceptibility of C. sordidus to plant cystatins, are discussed in the light of recent hypotheses suggesting a key role for cathepsin B-like enzymes as a determinant for resistance or susceptibility to plant cystatins in Coleoptera. SN - 1520-6327 UR - https://www.unboundmedicine.com/medline/citation/20035549/Deleterious_effects_of_plant_cystatins_against_the_banana_weevil_Cosmopolites_sordidus_ L2 - https://doi.org/10.1002/arch.20342 DB - PRIME DP - Unbound Medicine ER -