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Head-up tilt lowers IOP and improves RGC dysfunction in glaucomatous DBA/2J mice.
Exp Eye Res. 2010 Mar; 90(3):452-60.EE

Abstract

The inbred DBA/2J (D2) mouse strain is a well established model of spontaneously elevated intraocular pressure (IOP), progressive glaucomatous loss of retinal ganglion cells (RGCs), and early damage of RGC axons at the level of optic nerve head. Pattern electroretinogram (PERG) studies have shown that surviving RGCs in mice 6-12-month-old may be dysfunctional. RGC dysfunction seems to be IOP-dependent, since it may be exacerbated by means of acute IOP elevation with head-down body tilt. Here we test the hypothesis that head-up body posture lowers IOP, resulting in improvement of PERG amplitude in aged D2 mice with glaucoma. We show that head-up body tilt induces age-independent IOP lowering whose magnitude increases with the angle of tilt. For a fixed angle (-60 degrees) of head-up tilt, IOP progressively decreases with a time constant of about 5 min and stabilizes at a value lower by about 5-6 mm Hg compared to the baseline. Head-up tilt also results in an improvement of PERG amplitude in older D2 mice with glaucoma but not in younger D2 mice without glaucoma. Improvement of PERG amplitude in aged D2 mice upon head-up-induced IOP lowering is consistent with the idea that RGCs undergo a stage of IOP-dependent, reversible dysfunction before death. The head-up IOP/PERG protocol may represent a non-invasive way to probe the potential for recovery of RGC dysfunction in D2 mice.

Authors+Show Affiliations

Bascom Palmer Eye Institute, University of Miami Miller School of Medicine, 900 NW 17th Street, Miami, FL 33136, USA. vporciatti@med.miami.edu <vporciatti@med.miami.edu>No affiliation info available

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural

Language

eng

PubMed ID

20036238

Citation

Porciatti, Vittorio, and Mahesh Nagaraju. "Head-up Tilt Lowers IOP and Improves RGC Dysfunction in Glaucomatous DBA/2J Mice." Experimental Eye Research, vol. 90, no. 3, 2010, pp. 452-60.
Porciatti V, Nagaraju M. Head-up tilt lowers IOP and improves RGC dysfunction in glaucomatous DBA/2J mice. Exp Eye Res. 2010;90(3):452-60.
Porciatti, V., & Nagaraju, M. (2010). Head-up tilt lowers IOP and improves RGC dysfunction in glaucomatous DBA/2J mice. Experimental Eye Research, 90(3), 452-60. https://doi.org/10.1016/j.exer.2009.12.005
Porciatti V, Nagaraju M. Head-up Tilt Lowers IOP and Improves RGC Dysfunction in Glaucomatous DBA/2J Mice. Exp Eye Res. 2010;90(3):452-60. PubMed PMID: 20036238.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Head-up tilt lowers IOP and improves RGC dysfunction in glaucomatous DBA/2J mice. AU - Porciatti,Vittorio, AU - Nagaraju,Mahesh, Y1 - 2009/12/29/ PY - 2009/06/11/received PY - 2009/12/09/revised PY - 2009/12/14/accepted PY - 2009/12/29/entrez PY - 2009/12/29/pubmed PY - 2010/3/10/medline SP - 452 EP - 60 JF - Experimental eye research JO - Exp Eye Res VL - 90 IS - 3 N2 - The inbred DBA/2J (D2) mouse strain is a well established model of spontaneously elevated intraocular pressure (IOP), progressive glaucomatous loss of retinal ganglion cells (RGCs), and early damage of RGC axons at the level of optic nerve head. Pattern electroretinogram (PERG) studies have shown that surviving RGCs in mice 6-12-month-old may be dysfunctional. RGC dysfunction seems to be IOP-dependent, since it may be exacerbated by means of acute IOP elevation with head-down body tilt. Here we test the hypothesis that head-up body posture lowers IOP, resulting in improvement of PERG amplitude in aged D2 mice with glaucoma. We show that head-up body tilt induces age-independent IOP lowering whose magnitude increases with the angle of tilt. For a fixed angle (-60 degrees) of head-up tilt, IOP progressively decreases with a time constant of about 5 min and stabilizes at a value lower by about 5-6 mm Hg compared to the baseline. Head-up tilt also results in an improvement of PERG amplitude in older D2 mice with glaucoma but not in younger D2 mice without glaucoma. Improvement of PERG amplitude in aged D2 mice upon head-up-induced IOP lowering is consistent with the idea that RGCs undergo a stage of IOP-dependent, reversible dysfunction before death. The head-up IOP/PERG protocol may represent a non-invasive way to probe the potential for recovery of RGC dysfunction in D2 mice. SN - 1096-0007 UR - https://www.unboundmedicine.com/medline/citation/20036238/Head_up_tilt_lowers_IOP_and_improves_RGC_dysfunction_in_glaucomatous_DBA/2J_mice_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0014-4835(09)00352-2 DB - PRIME DP - Unbound Medicine ER -