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Bioactivity guided evaluation of anti-inflammatory and antinociceptive activities of Arceuthobium oxycedri (D.C.) M. Bieb.
J Ethnopharmacol. 2010 Mar 02; 128(1):79-84.JE

Abstract

ETHNOPHARMACOLOGICAL RELEVANCE

Arceuthobium oxycedri (D.C.) M. Bieb. (Loranthaceae) or dwarf mistletoe is a rare semi-parasitic plant that lives on the branches of Juniperus oxycedrus as the host plant. In Turkish folk medicine, the plant is prescribed as a panacea for every kind of diseases, including, against infectious and inflammatory disorders of upper respiratory system and gastro-intestinal complaints or as a hypotensive remedy.

AIM OF THE STUDY

In the current study, in vivo anti-inflammatory and antinociceptive effects of Arceuthobium oxycedri have been investigated.

MATERIAL AND METHODS

The crude ethanolic extract of the whole plant was sequentially fractionated into five subextracts; explicitly, n-hexane, chloroform, ethyl acetate (EtOAc), n-butanol, and remaining water extracts. Further studies were carried out on the most active subextract, i.e. the EtOAc subextract, was further subjected to fractionation through successive column chromatographic applications on Silica gel 60, Sephadex LH-20 and LiChropep RP-18. For the activity assessment, each extract or fraction was submitted to bioassay systems; carrageenan-induced hind paw edema model for anti-inflammatory activity and p-benzoquinone induced abdominal contraction test for antinociceptive activity assessment.

RESULTS

Among the extracts obtained, the ethanolic extract, EtOAc and n-butanol subextracts showed significant inhibitory activity in the bioassay systems. From the EtOAc subextract, a major component was isolated and its structure was determined as (+)-catechin by means of spectral techniques.

CONCLUSION

Present study confirms the claimed utilization of the plant against inflammatory complaints in Turkish folk medicine.

Authors+Show Affiliations

Department of Pharmacognosy, Faculty of Pharmacy, Gazi University, 06330 Ankara, Turkey.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20038452

Citation

Akkol, Esra Küpeli, et al. "Bioactivity Guided Evaluation of Anti-inflammatory and Antinociceptive Activities of Arceuthobium Oxycedri (D.C.) M. Bieb." Journal of Ethnopharmacology, vol. 128, no. 1, 2010, pp. 79-84.
Akkol EK, Orhan I, Kartal M, et al. Bioactivity guided evaluation of anti-inflammatory and antinociceptive activities of Arceuthobium oxycedri (D.C.) M. Bieb. J Ethnopharmacol. 2010;128(1):79-84.
Akkol, E. K., Orhan, I., Kartal, M., & Yeşilada, E. (2010). Bioactivity guided evaluation of anti-inflammatory and antinociceptive activities of Arceuthobium oxycedri (D.C.) M. Bieb. Journal of Ethnopharmacology, 128(1), 79-84. https://doi.org/10.1016/j.jep.2009.12.028
Akkol EK, et al. Bioactivity Guided Evaluation of Anti-inflammatory and Antinociceptive Activities of Arceuthobium Oxycedri (D.C.) M. Bieb. J Ethnopharmacol. 2010 Mar 2;128(1):79-84. PubMed PMID: 20038452.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Bioactivity guided evaluation of anti-inflammatory and antinociceptive activities of Arceuthobium oxycedri (D.C.) M. Bieb. AU - Akkol,Esra Küpeli, AU - Orhan,Ilkay, AU - Kartal,Murat, AU - Yeşilada,Erdem, Y1 - 2009/12/28/ PY - 2009/09/11/received PY - 2009/12/14/revised PY - 2009/12/17/accepted PY - 2009/12/30/entrez PY - 2009/12/30/pubmed PY - 2010/6/19/medline SP - 79 EP - 84 JF - Journal of ethnopharmacology JO - J Ethnopharmacol VL - 128 IS - 1 N2 - ETHNOPHARMACOLOGICAL RELEVANCE: Arceuthobium oxycedri (D.C.) M. Bieb. (Loranthaceae) or dwarf mistletoe is a rare semi-parasitic plant that lives on the branches of Juniperus oxycedrus as the host plant. In Turkish folk medicine, the plant is prescribed as a panacea for every kind of diseases, including, against infectious and inflammatory disorders of upper respiratory system and gastro-intestinal complaints or as a hypotensive remedy. AIM OF THE STUDY: In the current study, in vivo anti-inflammatory and antinociceptive effects of Arceuthobium oxycedri have been investigated. MATERIAL AND METHODS: The crude ethanolic extract of the whole plant was sequentially fractionated into five subextracts; explicitly, n-hexane, chloroform, ethyl acetate (EtOAc), n-butanol, and remaining water extracts. Further studies were carried out on the most active subextract, i.e. the EtOAc subextract, was further subjected to fractionation through successive column chromatographic applications on Silica gel 60, Sephadex LH-20 and LiChropep RP-18. For the activity assessment, each extract or fraction was submitted to bioassay systems; carrageenan-induced hind paw edema model for anti-inflammatory activity and p-benzoquinone induced abdominal contraction test for antinociceptive activity assessment. RESULTS: Among the extracts obtained, the ethanolic extract, EtOAc and n-butanol subextracts showed significant inhibitory activity in the bioassay systems. From the EtOAc subextract, a major component was isolated and its structure was determined as (+)-catechin by means of spectral techniques. CONCLUSION: Present study confirms the claimed utilization of the plant against inflammatory complaints in Turkish folk medicine. SN - 1872-7573 UR - https://www.unboundmedicine.com/medline/citation/20038452/Bioactivity_guided_evaluation_of_anti_inflammatory_and_antinociceptive_activities_of_Arceuthobium_oxycedri__D_C___M__Bieb_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0378-8741(09)00783-1 DB - PRIME DP - Unbound Medicine ER -