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Isolation and purification of macrocyclic components from Penicillium fermentation broth by high-speed counter-current chromatography.
J Sep Sci. 2010 Feb; 33(2):277-84.JS

Abstract

In this paper, high-speed counter-current chromatography (HSCCC), assisted with ESI-MS, was first successfully applied to the preparative separation of three macrolide antibiotics, brefeldin A (12.6 mg, 99.0%), 7'-O-formylbrefeldin A (6.5 mg, 95.0%) and 7'-O-acetylbrefeldin A (5.0 mg, 92.3%) from the crude extract of the microbe Penicillium SHZK-15. Considering the chemical nature and partition coefficient (K) values of the three target compounds, a two-step HSCCC isolation protocol was developed in order to obtain products with high purity. In the two-step method, the crude ethyl acetate extract was first fractionated and resulted in two peak fractions by HSCCC using solvent system n-hexane/ethyl acetate/methanol/water (HEMWat) (3:7:5:5 v/v/v/v), then purified using solvent systems HEMWat (3:5:3:5 v/v/v/v) and HEMWat (7:3:5:5 v/v/v/v) for each fraction. The purities and structures of the isolated compounds were determined by HPLC, X-ray crystallography, ESI-MS and NMR. The results demonstrated that HSCCC is a fast and efficient technique for systematic isolation of bioactive compounds from the microbes.

Authors+Show Affiliations

Department of Chemistry and The Key Laboratory for Chemical Biology of Fujian Province, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen, PR China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20041450

Citation

Gao, Xiang, et al. "Isolation and Purification of Macrocyclic Components From Penicillium Fermentation Broth By High-speed Counter-current Chromatography." Journal of Separation Science, vol. 33, no. 2, 2010, pp. 277-84.
Gao X, Zhuang R, Guo J, et al. Isolation and purification of macrocyclic components from Penicillium fermentation broth by high-speed counter-current chromatography. J Sep Sci. 2010;33(2):277-84.
Gao, X., Zhuang, R., Guo, J., Bao, J., Fang, M., Liu, Y., Xu, P., & Zhao, Y. (2010). Isolation and purification of macrocyclic components from Penicillium fermentation broth by high-speed counter-current chromatography. Journal of Separation Science, 33(2), 277-84. https://doi.org/10.1002/jssc.200900630
Gao X, et al. Isolation and Purification of Macrocyclic Components From Penicillium Fermentation Broth By High-speed Counter-current Chromatography. J Sep Sci. 2010;33(2):277-84. PubMed PMID: 20041450.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Isolation and purification of macrocyclic components from Penicillium fermentation broth by high-speed counter-current chromatography. AU - Gao,Xiang, AU - Zhuang,Rongqiang, AU - Guo,Jiannan, AU - Bao,Jian, AU - Fang,Meijuan, AU - Liu,Yan, AU - Xu,Pengxiang, AU - Zhao,Yufen, PY - 2009/12/31/entrez PY - 2009/12/31/pubmed PY - 2010/4/27/medline SP - 277 EP - 84 JF - Journal of separation science JO - J Sep Sci VL - 33 IS - 2 N2 - In this paper, high-speed counter-current chromatography (HSCCC), assisted with ESI-MS, was first successfully applied to the preparative separation of three macrolide antibiotics, brefeldin A (12.6 mg, 99.0%), 7'-O-formylbrefeldin A (6.5 mg, 95.0%) and 7'-O-acetylbrefeldin A (5.0 mg, 92.3%) from the crude extract of the microbe Penicillium SHZK-15. Considering the chemical nature and partition coefficient (K) values of the three target compounds, a two-step HSCCC isolation protocol was developed in order to obtain products with high purity. In the two-step method, the crude ethyl acetate extract was first fractionated and resulted in two peak fractions by HSCCC using solvent system n-hexane/ethyl acetate/methanol/water (HEMWat) (3:7:5:5 v/v/v/v), then purified using solvent systems HEMWat (3:5:3:5 v/v/v/v) and HEMWat (7:3:5:5 v/v/v/v) for each fraction. The purities and structures of the isolated compounds were determined by HPLC, X-ray crystallography, ESI-MS and NMR. The results demonstrated that HSCCC is a fast and efficient technique for systematic isolation of bioactive compounds from the microbes. SN - 1615-9314 UR - https://www.unboundmedicine.com/medline/citation/20041450/Isolation_and_purification_of_macrocyclic_components_from_Penicillium_fermentation_broth_by_high_speed_counter_current_chromatography_ L2 - https://doi.org/10.1002/jssc.200900630 DB - PRIME DP - Unbound Medicine ER -