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Identification of cardiac glycosides in fractions from Periploca forrestii by high-performance liquid chromatography/diode-array detection/electrospray ionization multi-stage tandem mass spectrometry and liquid chromatography/nuclear magnetic resonance.
J Chromatogr B Analyt Technol Biomed Life Sci. 2010 Feb 01; 878(3-4):381-90.JC

Abstract

Cardiac glycosides are a class of naturally occurring compounds that are characterized by some interesting biological activities and are widely distributed in the plant kingdom and can also be found in some animals. There is an interest in the chemical characterization of these molecules due to their toxicity and their use in medicines. In the study reported here, a combination of electrospray ionization tandem mass spectrometry with high-performance liquid chromatography equipped with diode-array detector (HPLC-DAD/ESI-MS(n)), and hyphenation to both liquid chromatography and nuclear magnetic resonance spectroscopy (HPLC/NMR) were utilized for the on-line analyses of cardiac glycosides from Periploca forrestii. The fragmentation patterns and (1)H NMR spectra of nine isolated cardiac glycosides were investigated; their fragmentation rules and (1)H NMR spectral characteristics were summarized and applied to the structural identification of similar constituents in fractions from P. forrestii. As a result, a total of nine trace cardiac glycosides were tentatively determined by analyses of accurate molecular masses, representative fragment ions and characteristic (1)H NMR signals provided by HPLC/high-resolution mass spectrometry (HRMS), HPLC-DAD/ESI-MS(n) and HPLC/(1)H NMR experiments, respectively. Of these, eight (2-9) are new compounds and one (1) is reported from P. forrestii for the first time. Results of the present study can benefit the rapid identification and targeted isolation of new cardiac glycosides from crude plant extracts.

Authors+Show Affiliations

Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine, Ministry of Education, Institute of Materia Medica, Peking Union Medical College & Chinese Academy of Medical Sciences, Beijing 100050, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20042379

Citation

Li, Yong, et al. "Identification of Cardiac Glycosides in Fractions From Periploca Forrestii By High-performance Liquid Chromatography/diode-array Detection/electrospray Ionization Multi-stage Tandem Mass Spectrometry and Liquid Chromatography/nuclear Magnetic Resonance." Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences, vol. 878, no. 3-4, 2010, pp. 381-90.
Li Y, Wu X, Li J, et al. Identification of cardiac glycosides in fractions from Periploca forrestii by high-performance liquid chromatography/diode-array detection/electrospray ionization multi-stage tandem mass spectrometry and liquid chromatography/nuclear magnetic resonance. J Chromatogr B Analyt Technol Biomed Life Sci. 2010;878(3-4):381-90.
Li, Y., Wu, X., Li, J., Wang, Y., Yu, S., Lv, H., Qu, J., Abliz, Z., Liu, J., Liu, Y., & Du, D. (2010). Identification of cardiac glycosides in fractions from Periploca forrestii by high-performance liquid chromatography/diode-array detection/electrospray ionization multi-stage tandem mass spectrometry and liquid chromatography/nuclear magnetic resonance. Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences, 878(3-4), 381-90. https://doi.org/10.1016/j.jchromb.2009.12.008
Li Y, et al. Identification of Cardiac Glycosides in Fractions From Periploca Forrestii By High-performance Liquid Chromatography/diode-array Detection/electrospray Ionization Multi-stage Tandem Mass Spectrometry and Liquid Chromatography/nuclear Magnetic Resonance. J Chromatogr B Analyt Technol Biomed Life Sci. 2010 Feb 1;878(3-4):381-90. PubMed PMID: 20042379.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Identification of cardiac glycosides in fractions from Periploca forrestii by high-performance liquid chromatography/diode-array detection/electrospray ionization multi-stage tandem mass spectrometry and liquid chromatography/nuclear magnetic resonance. AU - Li,Yong, AU - Wu,Xianfu, AU - Li,Jianbei, AU - Wang,Yinghong, AU - Yu,Shishan, AU - Lv,Haining, AU - Qu,Jing, AU - Abliz,Zeper, AU - Liu,Jing, AU - Liu,Yuanyan, AU - Du,Dan, Y1 - 2009/12/16/ PY - 2009/07/01/received PY - 2009/12/02/revised PY - 2009/12/08/accepted PY - 2010/1/1/entrez PY - 2010/1/1/pubmed PY - 2010/5/19/medline SP - 381 EP - 90 JF - Journal of chromatography. B, Analytical technologies in the biomedical and life sciences JO - J Chromatogr B Analyt Technol Biomed Life Sci VL - 878 IS - 3-4 N2 - Cardiac glycosides are a class of naturally occurring compounds that are characterized by some interesting biological activities and are widely distributed in the plant kingdom and can also be found in some animals. There is an interest in the chemical characterization of these molecules due to their toxicity and their use in medicines. In the study reported here, a combination of electrospray ionization tandem mass spectrometry with high-performance liquid chromatography equipped with diode-array detector (HPLC-DAD/ESI-MS(n)), and hyphenation to both liquid chromatography and nuclear magnetic resonance spectroscopy (HPLC/NMR) were utilized for the on-line analyses of cardiac glycosides from Periploca forrestii. The fragmentation patterns and (1)H NMR spectra of nine isolated cardiac glycosides were investigated; their fragmentation rules and (1)H NMR spectral characteristics were summarized and applied to the structural identification of similar constituents in fractions from P. forrestii. As a result, a total of nine trace cardiac glycosides were tentatively determined by analyses of accurate molecular masses, representative fragment ions and characteristic (1)H NMR signals provided by HPLC/high-resolution mass spectrometry (HRMS), HPLC-DAD/ESI-MS(n) and HPLC/(1)H NMR experiments, respectively. Of these, eight (2-9) are new compounds and one (1) is reported from P. forrestii for the first time. Results of the present study can benefit the rapid identification and targeted isolation of new cardiac glycosides from crude plant extracts. SN - 1873-376X UR - https://www.unboundmedicine.com/medline/citation/20042379/Identification_of_cardiac_glycosides_in_fractions_from_Periploca_forrestii_by_high_performance_liquid_chromatography/diode_array_detection/electrospray_ionization_multi_stage_tandem_mass_spectrometry_and_liquid_chromatography/nuclear_magnetic_resonance_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1570-0232(09)00859-9 DB - PRIME DP - Unbound Medicine ER -