Tags

Type your tag names separated by a space and hit enter

IL-17A and IL-17F stimulate chemokines via MAPK pathways (ERK1/2 and p38 but not JNK) in mouse cultured mesangial cells: synergy with TNF-alpha and IL-1beta.
Am J Physiol Renal Physiol. 2010 Mar; 298(3):F779-87.AJ

Abstract

We investigated the role of IL-17 family members IL-17A and IL-17F in the induction of chemokines in mouse cultured mesangial cells (SV40 MES 13 cells). We evaluated the expression of the chemokines monocyte chemoattractant protein-1 (MCP-1) and macrophage inflammatory protein-2 (MIP-2) by ELISA and real-time RT-PCR (Q-PCR). Activation of MAPK was assessed by immunoblotting. IL-17RA and IL-17RC were inhibited by small interfering RNA (siRNA). We found that IL-17A or IL-17F stimulation of mesangial cells led to both a dose- and time-dependent increase in MCP-1 and MIP-2 release. This effect was dependent on mRNA transcription and protein translation. Both also enhanced TNF-alpha- and IL-1beta-mediated MCP-1 and MIP-2 release in the cells. Additionally, we observed that IL-17A and IL-17F induced MAPK (p38 MAPK, ERK1/2, and JNK) activation and that pharmacological inhibitors of p38 MAPK (SB203580) and ERK1/2 (U0126), but not JNK (SP600125), blocked the IL-17A/IL-17F-mediated MCP-1 and MIP-2 release. Mesangial cells expressed IL-17RA and IL-17RC, and the IL-17A-mediated MCP-1 and MIP-2 release was significantly blocked by soluble IL-17RA. Furthermore, inhibition of either IL-17RA or IL-17RC expression via siRNA led to significant reduction of IL-17A/IL-17F-stimulated chemokine production. We conclude that IL-17A and IL-17F induce the production of chemokines MCP-1 and MIP-2 via MAPK pathways (p38 MAPK and ERK1/2), as well as mRNA transcription and protein translation and have synergistic effects with TNF-alpha and IL-1beta in cultured mesangial cells.

Authors+Show Affiliations

Division of Nephrology, Department of Medicine, Showa University School of Medicine, Tokyo, Japan. iyoda@med.showa-u.ac.jpNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20042461

Citation

Iyoda, Masayuki, et al. "IL-17A and IL-17F Stimulate Chemokines Via MAPK Pathways (ERK1/2 and P38 but Not JNK) in Mouse Cultured Mesangial Cells: Synergy With TNF-alpha and IL-1beta." American Journal of Physiology. Renal Physiology, vol. 298, no. 3, 2010, pp. F779-87.
Iyoda M, Shibata T, Kawaguchi M, et al. IL-17A and IL-17F stimulate chemokines via MAPK pathways (ERK1/2 and p38 but not JNK) in mouse cultured mesangial cells: synergy with TNF-alpha and IL-1beta. Am J Physiol Renal Physiol. 2010;298(3):F779-87.
Iyoda, M., Shibata, T., Kawaguchi, M., Hizawa, N., Yamaoka, T., Kokubu, F., & Akizawa, T. (2010). IL-17A and IL-17F stimulate chemokines via MAPK pathways (ERK1/2 and p38 but not JNK) in mouse cultured mesangial cells: synergy with TNF-alpha and IL-1beta. American Journal of Physiology. Renal Physiology, 298(3), F779-87. https://doi.org/10.1152/ajprenal.00198.2009
Iyoda M, et al. IL-17A and IL-17F Stimulate Chemokines Via MAPK Pathways (ERK1/2 and P38 but Not JNK) in Mouse Cultured Mesangial Cells: Synergy With TNF-alpha and IL-1beta. Am J Physiol Renal Physiol. 2010;298(3):F779-87. PubMed PMID: 20042461.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - IL-17A and IL-17F stimulate chemokines via MAPK pathways (ERK1/2 and p38 but not JNK) in mouse cultured mesangial cells: synergy with TNF-alpha and IL-1beta. AU - Iyoda,Masayuki, AU - Shibata,Takanori, AU - Kawaguchi,Mio, AU - Hizawa,Nobuyuki, AU - Yamaoka,Toshimitsu, AU - Kokubu,Fumio, AU - Akizawa,Tadao, Y1 - 2009/12/30/ PY - 2010/1/1/entrez PY - 2010/1/1/pubmed PY - 2010/3/20/medline SP - F779 EP - 87 JF - American journal of physiology. Renal physiology JO - Am J Physiol Renal Physiol VL - 298 IS - 3 N2 - We investigated the role of IL-17 family members IL-17A and IL-17F in the induction of chemokines in mouse cultured mesangial cells (SV40 MES 13 cells). We evaluated the expression of the chemokines monocyte chemoattractant protein-1 (MCP-1) and macrophage inflammatory protein-2 (MIP-2) by ELISA and real-time RT-PCR (Q-PCR). Activation of MAPK was assessed by immunoblotting. IL-17RA and IL-17RC were inhibited by small interfering RNA (siRNA). We found that IL-17A or IL-17F stimulation of mesangial cells led to both a dose- and time-dependent increase in MCP-1 and MIP-2 release. This effect was dependent on mRNA transcription and protein translation. Both also enhanced TNF-alpha- and IL-1beta-mediated MCP-1 and MIP-2 release in the cells. Additionally, we observed that IL-17A and IL-17F induced MAPK (p38 MAPK, ERK1/2, and JNK) activation and that pharmacological inhibitors of p38 MAPK (SB203580) and ERK1/2 (U0126), but not JNK (SP600125), blocked the IL-17A/IL-17F-mediated MCP-1 and MIP-2 release. Mesangial cells expressed IL-17RA and IL-17RC, and the IL-17A-mediated MCP-1 and MIP-2 release was significantly blocked by soluble IL-17RA. Furthermore, inhibition of either IL-17RA or IL-17RC expression via siRNA led to significant reduction of IL-17A/IL-17F-stimulated chemokine production. We conclude that IL-17A and IL-17F induce the production of chemokines MCP-1 and MIP-2 via MAPK pathways (p38 MAPK and ERK1/2), as well as mRNA transcription and protein translation and have synergistic effects with TNF-alpha and IL-1beta in cultured mesangial cells. SN - 1522-1466 UR - https://www.unboundmedicine.com/medline/citation/20042461/IL_17A_and_IL_17F_stimulate_chemokines_via_MAPK_pathways__ERK1/2_and_p38_but_not_JNK__in_mouse_cultured_mesangial_cells:_synergy_with_TNF_alpha_and_IL_1beta_ L2 - https://journals.physiology.org/doi/10.1152/ajprenal.00198.2009?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -