Tags

Type your tag names separated by a space and hit enter

Deamidated gliadin peptide antibodies as a routine test for celiac disease: a prospective analysis.
J Clin Gastroenterol. 2010 Mar; 44(3):186-90.JC

Abstract

GOALS

This study was designed to establish whether deamidated gliadin peptide antibodies (DGP-AGA) could improve the serologic workup for celiac disease (CD).

BACKGROUND

The best serologic approach for CD screening is currently based on the combined detection of tissue transglutaminase (tTGA), endomysial (EmA), and gliadin antibodies (AGA).

STUDY

One hundred forty-four consecutive patients with gastrointestinal and extraintestinal signs suggestive for CD were investigated using serologic tests, that is, IgG and IgA DGP-AGA, IgA tTGA, IgA EmA, and duodenal biopsy.

RESULTS

Forty-eight out of 144 patients (33%) had CD with different severity of villous atrophy. IgA tTGA showed 93.7% sensitivity compared with 91.6% for IgA EmA, 84.3% for IgA DGP-AGA, and 82.3% for IgG DGP-AGA. Of the 3 cases negative for IgA tTGA, IgA EmA, and IgA DGP-AGA, 2 had total IgA deficiency, although both were positive for IgG DGP-AGA. IgG DGP-AGA showed a very high specificity for CD (98.9%), not only superior to IgA DGP-AGA (79.8%), but also to IgA tTGA (96.6%) and very close to IgA EmA (100%).

CONCLUSIONS

Our prospective study shows that the combined search for IgA tTGA and IgG DGP-AGA provides the best diagnostic accuracy for CD, allowing the identification of all CD cases---except one---with a very high specificity. The serologic workup for CD screening could be significantly improved by the routine introduction of IgG DGP-AGA together with IgA tTGA, thus reducing the number of tests and with an obvious advantage in terms of cost-efficacy.

Authors+Show Affiliations

Department of Clinical Medicine, University of Bologna, Bologna, Italy. umberto.volta@aosp.bo.itNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20042872

Citation

Volta, Umberto, et al. "Deamidated Gliadin Peptide Antibodies as a Routine Test for Celiac Disease: a Prospective Analysis." Journal of Clinical Gastroenterology, vol. 44, no. 3, 2010, pp. 186-90.
Volta U, Granito A, Parisi C, et al. Deamidated gliadin peptide antibodies as a routine test for celiac disease: a prospective analysis. J Clin Gastroenterol. 2010;44(3):186-90.
Volta, U., Granito, A., Parisi, C., Fabbri, A., Fiorini, E., Piscaglia, M., Tovoli, F., Grasso, V., Muratori, P., Pappas, G., & De Giorgio, R. (2010). Deamidated gliadin peptide antibodies as a routine test for celiac disease: a prospective analysis. Journal of Clinical Gastroenterology, 44(3), 186-90. https://doi.org/10.1097/MCG.0b013e3181c378f6
Volta U, et al. Deamidated Gliadin Peptide Antibodies as a Routine Test for Celiac Disease: a Prospective Analysis. J Clin Gastroenterol. 2010;44(3):186-90. PubMed PMID: 20042872.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Deamidated gliadin peptide antibodies as a routine test for celiac disease: a prospective analysis. AU - Volta,Umberto, AU - Granito,Alessandro, AU - Parisi,Claudia, AU - Fabbri,Angela, AU - Fiorini,Erica, AU - Piscaglia,Maria, AU - Tovoli,Francesco, AU - Grasso,Valentina, AU - Muratori,Paolo, AU - Pappas,Georgios, AU - De Giorgio,Roberto, PY - 2010/1/1/entrez PY - 2010/1/1/pubmed PY - 2010/5/21/medline SP - 186 EP - 90 JF - Journal of clinical gastroenterology JO - J. Clin. Gastroenterol. VL - 44 IS - 3 N2 - GOALS: This study was designed to establish whether deamidated gliadin peptide antibodies (DGP-AGA) could improve the serologic workup for celiac disease (CD). BACKGROUND: The best serologic approach for CD screening is currently based on the combined detection of tissue transglutaminase (tTGA), endomysial (EmA), and gliadin antibodies (AGA). STUDY: One hundred forty-four consecutive patients with gastrointestinal and extraintestinal signs suggestive for CD were investigated using serologic tests, that is, IgG and IgA DGP-AGA, IgA tTGA, IgA EmA, and duodenal biopsy. RESULTS: Forty-eight out of 144 patients (33%) had CD with different severity of villous atrophy. IgA tTGA showed 93.7% sensitivity compared with 91.6% for IgA EmA, 84.3% for IgA DGP-AGA, and 82.3% for IgG DGP-AGA. Of the 3 cases negative for IgA tTGA, IgA EmA, and IgA DGP-AGA, 2 had total IgA deficiency, although both were positive for IgG DGP-AGA. IgG DGP-AGA showed a very high specificity for CD (98.9%), not only superior to IgA DGP-AGA (79.8%), but also to IgA tTGA (96.6%) and very close to IgA EmA (100%). CONCLUSIONS: Our prospective study shows that the combined search for IgA tTGA and IgG DGP-AGA provides the best diagnostic accuracy for CD, allowing the identification of all CD cases---except one---with a very high specificity. The serologic workup for CD screening could be significantly improved by the routine introduction of IgG DGP-AGA together with IgA tTGA, thus reducing the number of tests and with an obvious advantage in terms of cost-efficacy. SN - 1539-2031 UR - https://www.unboundmedicine.com/medline/citation/20042872/Deamidated_gliadin_peptide_antibodies_as_a_routine_test_for_celiac_disease:_a_prospective_analysis_ L2 - http://dx.doi.org/10.1097/MCG.0b013e3181c378f6 DB - PRIME DP - Unbound Medicine ER -