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IL-17A suppresses the expression of bone resorption-related proteinases and osteoclast differentiation via IL-17RA or IL-17RC receptors in RAW264.7 cells.
Biochimie. 2010 Apr; 92(4):398-404.B

Abstract

Interleukin-17 (IL-17) is produced exclusively by activated T cells and neutrophils, and stimulates osteoclastic bone resorption via osteoblasts by inducing the expression of "receptor activator of NF-kappaB (RANK) ligand" (RANKL). However, the direct effects of IL-17 on the differentiation of osteoclast precursors into osteoclasts and on the function of osteoclasts have not been clarified. Therefore, we examined the effects of IL-17A on the differentiation of osteoclast precursors using RAW264.7 cells and also on the expression of carbonic anhydrase II (CA II), cathepsin K, matrix metalloproteinases-9 (MMP-9), RANK, c-fms, and IL-17 receptors in these cells. The cells were cultured with or without 0.1, 1.0, 10 or 50 ng/mL IL-17 in the presence of soluble RANKL for up to 10 days. The CA II, cathepsin K, and MMP-9 mRNA and protein expression levels were examined using real-time PCR and Western blotting, respectively. The mRNA expression levels of RANK, c-fms, and IL-17 receptors were monitored by real-time PCR. Osteoclast differentiation was estimated using tartrate-resistant acid phosphatase (TRAP) staining of the cells. TRAP-positive cells were observed after day 5 of culture, and the number of cells decreased in the presence of 10 and 50 ng/mL IL-17A at days 5 and 7. In the presence of IL-17A, the expressions of cathepsin K, MMP-9 and c-fms decreased markedly on days 5 and/or 7 of culture, whereas the expression of CA II and IL-17 receptor (type A) increased remarkably at days 3 and 7, respectively. The expression of RANK and IL-17 receptor (type C) was not affected by the addition of IL-17A. These results suggest that the differentiation of osteoclast precursors into osteoclasts is suppressed at high concentrations of IL-17A. Furthermore, IL-17A suppresses the hydrolysis of matrix proteins during bone resorption by decreasing the production of cathepsin K and MMP-9 in osteoclasts.

Authors+Show Affiliations

Department of Oral Health Sciences, Nihon University School of Dentistry, 1-8-13 Kanda Surugadai, Chiyoda-ku, Tokyo, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

20045440

Citation

Kitami, Satoshi, et al. "IL-17A Suppresses the Expression of Bone Resorption-related Proteinases and Osteoclast Differentiation Via IL-17RA or IL-17RC Receptors in RAW264.7 Cells." Biochimie, vol. 92, no. 4, 2010, pp. 398-404.
Kitami S, Tanaka H, Kawato T, et al. IL-17A suppresses the expression of bone resorption-related proteinases and osteoclast differentiation via IL-17RA or IL-17RC receptors in RAW264.7 cells. Biochimie. 2010;92(4):398-404.
Kitami, S., Tanaka, H., Kawato, T., Tanabe, N., Katono-Tani, T., Zhang, F., Suzuki, N., Yonehara, Y., & Maeno, M. (2010). IL-17A suppresses the expression of bone resorption-related proteinases and osteoclast differentiation via IL-17RA or IL-17RC receptors in RAW264.7 cells. Biochimie, 92(4), 398-404. https://doi.org/10.1016/j.biochi.2009.12.011
Kitami S, et al. IL-17A Suppresses the Expression of Bone Resorption-related Proteinases and Osteoclast Differentiation Via IL-17RA or IL-17RC Receptors in RAW264.7 Cells. Biochimie. 2010;92(4):398-404. PubMed PMID: 20045440.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - IL-17A suppresses the expression of bone resorption-related proteinases and osteoclast differentiation via IL-17RA or IL-17RC receptors in RAW264.7 cells. AU - Kitami,Satoshi, AU - Tanaka,Hideki, AU - Kawato,Takayuki, AU - Tanabe,Natsuko, AU - Katono-Tani,Tomoko, AU - Zhang,Fan, AU - Suzuki,Naoto, AU - Yonehara,Yoshiyuki, AU - Maeno,Masao, Y1 - 2010/01/04/ PY - 2009/08/04/received PY - 2009/12/18/accepted PY - 2010/1/5/entrez PY - 2010/1/5/pubmed PY - 2010/6/9/medline SP - 398 EP - 404 JF - Biochimie JO - Biochimie VL - 92 IS - 4 N2 - Interleukin-17 (IL-17) is produced exclusively by activated T cells and neutrophils, and stimulates osteoclastic bone resorption via osteoblasts by inducing the expression of "receptor activator of NF-kappaB (RANK) ligand" (RANKL). However, the direct effects of IL-17 on the differentiation of osteoclast precursors into osteoclasts and on the function of osteoclasts have not been clarified. Therefore, we examined the effects of IL-17A on the differentiation of osteoclast precursors using RAW264.7 cells and also on the expression of carbonic anhydrase II (CA II), cathepsin K, matrix metalloproteinases-9 (MMP-9), RANK, c-fms, and IL-17 receptors in these cells. The cells were cultured with or without 0.1, 1.0, 10 or 50 ng/mL IL-17 in the presence of soluble RANKL for up to 10 days. The CA II, cathepsin K, and MMP-9 mRNA and protein expression levels were examined using real-time PCR and Western blotting, respectively. The mRNA expression levels of RANK, c-fms, and IL-17 receptors were monitored by real-time PCR. Osteoclast differentiation was estimated using tartrate-resistant acid phosphatase (TRAP) staining of the cells. TRAP-positive cells were observed after day 5 of culture, and the number of cells decreased in the presence of 10 and 50 ng/mL IL-17A at days 5 and 7. In the presence of IL-17A, the expressions of cathepsin K, MMP-9 and c-fms decreased markedly on days 5 and/or 7 of culture, whereas the expression of CA II and IL-17 receptor (type A) increased remarkably at days 3 and 7, respectively. The expression of RANK and IL-17 receptor (type C) was not affected by the addition of IL-17A. These results suggest that the differentiation of osteoclast precursors into osteoclasts is suppressed at high concentrations of IL-17A. Furthermore, IL-17A suppresses the hydrolysis of matrix proteins during bone resorption by decreasing the production of cathepsin K and MMP-9 in osteoclasts. SN - 1638-6183 UR - https://www.unboundmedicine.com/medline/citation/20045440/IL_17A_suppresses_the_expression_of_bone_resorption_related_proteinases_and_osteoclast_differentiation_via_IL_17RA_or_IL_17RC_receptors_in_RAW264_7_cells_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0300-9084(09)00352-6 DB - PRIME DP - Unbound Medicine ER -