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Differences in gene expression dependent on sampling site in placental tissue of fetuses with intrauterine growth restriction.
Placenta 2010; 31(3):178-85P

Abstract

OBJECTIVE

The human placenta as part of the feto-placental unit may influence fetal endocrine systems and may therefore represent a very important link between intrauterine growth restriction (IUGR) and metabolic disorders in later life. We aimed to analyze the effect of sample origin on gene expression of placental factors potentially involved in fetal programming in IUGR versus appropriate for gestational age growth (AGA) to standardize sample collection procedure for a multicenter approach.

DESIGN

Placental gene expression of insulin-like growth factor-binding protein (IGFBP)-1, prolactin, corticotropin releasing hormone (CRH) and leptin was measured and compared between proximal, intermediate and peripheral region of the placenta in 22 IUGR (proven by anomalous placental Doppler velocimetry) and 19 AGA neonates.

RESULTS

Whereas no difference in gene expression was seen in the proximal portion, in the intermediate placental region mRNA expression of IGFBP-1 (p = 0.01), prolactin (p = 0.04), CRH (p = 0.01) and leptin (p = 0.04) was increased in IUGR samples compared to controls. At the placental periphery, gene expression of these placental transcripts showed a higher expression level in IUGR placentas without statistical significance, except for leptin (p = 0.03).

CONCLUSION

Placental sampling site seems to be relevant for detecting differences in gene expression between IUGR and AGA neonates.

Authors+Show Affiliations

Department of Pediatrics and Adolescent Medicine, University of Erlangen-Nuremberg, Erlangen, Germany.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20045557

Citation

Tzschoppe, A A., et al. "Differences in Gene Expression Dependent On Sampling Site in Placental Tissue of Fetuses With Intrauterine Growth Restriction." Placenta, vol. 31, no. 3, 2010, pp. 178-85.
Tzschoppe AA, Struwe E, Dörr HG, et al. Differences in gene expression dependent on sampling site in placental tissue of fetuses with intrauterine growth restriction. Placenta. 2010;31(3):178-85.
Tzschoppe, A. A., Struwe, E., Dörr, H. G., Goecke, T. W., Beckmann, M. W., Schild, R. L., & Dötsch, J. (2010). Differences in gene expression dependent on sampling site in placental tissue of fetuses with intrauterine growth restriction. Placenta, 31(3), pp. 178-85. doi:10.1016/j.placenta.2009.12.002.
Tzschoppe AA, et al. Differences in Gene Expression Dependent On Sampling Site in Placental Tissue of Fetuses With Intrauterine Growth Restriction. Placenta. 2010;31(3):178-85. PubMed PMID: 20045557.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Differences in gene expression dependent on sampling site in placental tissue of fetuses with intrauterine growth restriction. AU - Tzschoppe,A A, AU - Struwe,E, AU - Dörr,H G, AU - Goecke,T W, AU - Beckmann,M W, AU - Schild,R L, AU - Dötsch,J, Y1 - 2010/01/04/ PY - 2009/08/15/received PY - 2009/11/21/revised PY - 2009/12/03/accepted PY - 2010/1/5/entrez PY - 2010/1/5/pubmed PY - 2010/5/25/medline SP - 178 EP - 85 JF - Placenta JO - Placenta VL - 31 IS - 3 N2 - OBJECTIVE: The human placenta as part of the feto-placental unit may influence fetal endocrine systems and may therefore represent a very important link between intrauterine growth restriction (IUGR) and metabolic disorders in later life. We aimed to analyze the effect of sample origin on gene expression of placental factors potentially involved in fetal programming in IUGR versus appropriate for gestational age growth (AGA) to standardize sample collection procedure for a multicenter approach. DESIGN: Placental gene expression of insulin-like growth factor-binding protein (IGFBP)-1, prolactin, corticotropin releasing hormone (CRH) and leptin was measured and compared between proximal, intermediate and peripheral region of the placenta in 22 IUGR (proven by anomalous placental Doppler velocimetry) and 19 AGA neonates. RESULTS: Whereas no difference in gene expression was seen in the proximal portion, in the intermediate placental region mRNA expression of IGFBP-1 (p = 0.01), prolactin (p = 0.04), CRH (p = 0.01) and leptin (p = 0.04) was increased in IUGR samples compared to controls. At the placental periphery, gene expression of these placental transcripts showed a higher expression level in IUGR placentas without statistical significance, except for leptin (p = 0.03). CONCLUSION: Placental sampling site seems to be relevant for detecting differences in gene expression between IUGR and AGA neonates. SN - 1532-3102 UR - https://www.unboundmedicine.com/medline/citation/20045557/Differences_in_gene_expression_dependent_on_sampling_site_in_placental_tissue_of_fetuses_with_intrauterine_growth_restriction_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0143-4004(09)00392-0 DB - PRIME DP - Unbound Medicine ER -