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Inhibition of xanthine oxidase by Acacia confusa extracts and their phytochemicals.
J Agric Food Chem. 2010 Jan 27; 58(2):781-6.JA

Abstract

Acacia confusa Merr. (Leguminosae) is traditionally used as a medicinal plant in Taiwan. In the present study, the XOD-inhibitory activity of ethanolic extracts from A. confusa was investigated for the first time. Results demonstrated that the ethanolic extract of A. confusa heartwood had a strong XOD-inhibitory activity. Among all fractions derived from heartwood extracts, the EtOAc fraction exhibited the best inhibitory activity. Following column chromatography and reverse-phase high-performance liquid chromatography, eight specific phytochemicals including melanoxetin, 7,8,3',4'-tetrahydroxyflavone, transilitin, okanin, 3,7,8,3'-tetrahydroxy-4'-methoxyflavone, 7,8,3'-trihydroxy-3,4'-dimethoxyflavone, 7,3',4'-trihydroxyflavone, and 7,3',4'-trihydroxy-3-methoxyflavone were isolated and identified from the EtOAc fraction. In addition, the IC(50) values indicated that okanin showed the strongest XOD-inhibitory effect (IC(50) value of 0.076 microM), followed by melanoxetin (0.274 microM) and allopurinol (4.784 microM). The present study revealed that okanin and melanoxetin showed excellent inhibition on XOD in noncompetitive and competitive mode, respectively, and their inhibitory activity is better than that of allopurinol. This is the first study that demonstrates the XOD-inhibitory performance of okanin and melanoxetin.

Authors+Show Affiliations

School of Forestry and Resource Conservation, National Taiwan University, Taipei 106, Taiwan.No affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20047272

Citation

Tung, Yu-Tang, and Shang-Tzen Chang. "Inhibition of Xanthine Oxidase By Acacia Confusa Extracts and Their Phytochemicals." Journal of Agricultural and Food Chemistry, vol. 58, no. 2, 2010, pp. 781-6.
Tung YT, Chang ST. Inhibition of xanthine oxidase by Acacia confusa extracts and their phytochemicals. J Agric Food Chem. 2010;58(2):781-6.
Tung, Y. T., & Chang, S. T. (2010). Inhibition of xanthine oxidase by Acacia confusa extracts and their phytochemicals. Journal of Agricultural and Food Chemistry, 58(2), 781-6. https://doi.org/10.1021/jf901498q
Tung YT, Chang ST. Inhibition of Xanthine Oxidase By Acacia Confusa Extracts and Their Phytochemicals. J Agric Food Chem. 2010 Jan 27;58(2):781-6. PubMed PMID: 20047272.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Inhibition of xanthine oxidase by Acacia confusa extracts and their phytochemicals. AU - Tung,Yu-Tang, AU - Chang,Shang-Tzen, PY - 2010/1/6/entrez PY - 2010/1/6/pubmed PY - 2010/3/26/medline SP - 781 EP - 6 JF - Journal of agricultural and food chemistry JO - J. Agric. Food Chem. VL - 58 IS - 2 N2 - Acacia confusa Merr. (Leguminosae) is traditionally used as a medicinal plant in Taiwan. In the present study, the XOD-inhibitory activity of ethanolic extracts from A. confusa was investigated for the first time. Results demonstrated that the ethanolic extract of A. confusa heartwood had a strong XOD-inhibitory activity. Among all fractions derived from heartwood extracts, the EtOAc fraction exhibited the best inhibitory activity. Following column chromatography and reverse-phase high-performance liquid chromatography, eight specific phytochemicals including melanoxetin, 7,8,3',4'-tetrahydroxyflavone, transilitin, okanin, 3,7,8,3'-tetrahydroxy-4'-methoxyflavone, 7,8,3'-trihydroxy-3,4'-dimethoxyflavone, 7,3',4'-trihydroxyflavone, and 7,3',4'-trihydroxy-3-methoxyflavone were isolated and identified from the EtOAc fraction. In addition, the IC(50) values indicated that okanin showed the strongest XOD-inhibitory effect (IC(50) value of 0.076 microM), followed by melanoxetin (0.274 microM) and allopurinol (4.784 microM). The present study revealed that okanin and melanoxetin showed excellent inhibition on XOD in noncompetitive and competitive mode, respectively, and their inhibitory activity is better than that of allopurinol. This is the first study that demonstrates the XOD-inhibitory performance of okanin and melanoxetin. SN - 1520-5118 UR - https://www.unboundmedicine.com/medline/citation/20047272/Inhibition_of_xanthine_oxidase_by_Acacia_confusa_extracts_and_their_phytochemicals_ L2 - https://dx.doi.org/10.1021/jf901498q DB - PRIME DP - Unbound Medicine ER -
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