TY - JOUR T1 - Hydrophilic interaction liquid chromatography-tandem mass spectrometry (HILIC-MS/MS) determination of cocaine and its metabolites benzoylecgonine, ecgonine methyl ester, and cocaethylene in hair samples. AU - Quintela,Oscar, AU - Lendoiro,Elena, AU - Cruz,Angelines, AU - de Castro,Ana, AU - Quevedo,Alfredo, AU - Jurado,Carmen, AU - López-Rivadulla,Manuel, Y1 - 2010/01/06/ PY - 2009/09/18/received PY - 2009/12/08/accepted PY - 2009/11/30/revised PY - 2010/1/7/entrez PY - 2010/1/7/pubmed PY - 2011/2/10/medline SP - 1703 EP - 12 JF - Analytical and bioanalytical chemistry JO - Anal Bioanal Chem VL - 396 IS - 5 N2 - This study reports the development and validation of a method using hydrophilic interaction liquid chromatography-tandem mass spectrometry (HILIC-MS/MS) for the analysis of cocaine and its metabolites benzoylecgonine (BE), ecgonine methyl ester (EME), and cocaethylene (CE) in hair samples. Decontamination was performed as follows: Firstly, the aliquot of hair was briefly rinsed with 2 mL dichloromethane, then was washed three times with 10 mL 0.01 M phosphate buffer, pH 6, for 15 min, followed by 2 mL 2-propanol for less than 2 min, and, finally, a last rinse with 2 mL dichloromethane was again done. Cocaine compounds were extracted from 10 mg of hair by incubation with 2 mL 0.1 M HCl at 50 °C for 12 h and purified by solid phase extraction with Oasis MCX cartridges. Analysis was performed by LC-MS/MS using an Atlantis HILIC silica chromatographic column. The method was fully validated. Linearity was established over the concentration range 0.020-10.0 ng/mg for cocaine (COC), 0.010-10.0 ng/mg for BE and CE, and 0.005-2.0 ng/mg for EME, and the correlation coefficients were all >0.99. Extraction efficiency was >70% for all analytes. Limits of detection were 0.0005 ng/mg for CE and 0.001 ng/mg for the other analytes (COC, BE, and EME). Lower limits of quantification were the lowest points of the calibration curves with acceptable accuracy and precision (coefficient of variation ≤20%). Intra- and inter-day imprecision ranged between 1.5% and 9.5% and 0.7% and 12.6%, respectively. Intra- and inter-day inaccuracy ranged from 0.5% to 12.3% and from 0.7% to 7.1%, respectively. With regard to matrix effects, suppression was <27.5% in all cases. The method was applied to the analysis of several samples derived from forensic cases. SN - 1618-2650 UR - https://www.unboundmedicine.com/medline/citation/20052577/Hydrophilic_interaction_liquid_chromatography_tandem_mass_spectrometry__HILIC_MS/MS__determination_of_cocaine_and_its_metabolites_benzoylecgonine_ecgonine_methyl_ester_and_cocaethylene_in_hair_samples_ L2 - https://dx.doi.org/10.1007/s00216-009-3393-5 DB - PRIME DP - Unbound Medicine ER -