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Molecular characterization of extended-spectrum beta-lactamases in clinical Escherichia coli and Klebsiella pneumoniae isolates from Surabaya, Indonesia.
J Antimicrob Chemother. 2010 Mar; 65(3):465-9.JA

Abstract

BACKGROUND

No detailed reports regarding extended-spectrum beta-lactamase (ESBL)-producing Enterobacteriaceae are currently available from Indonesia, the fourth most populous country in the world.

METHODS

A survey was carried out to investigate the molecular epidemiology and genetic characteristics of clinical ESBL-producing Escherichia coli and Klebsiella pneumoniae isolates originating from the Dr. Soetomo Academic Hospital in Surabaya, Indonesia, over a 4 month period (January to April 2005). ESBLs were characterized by isoelectric focusing and PCR assays. Clonality of the isolates was assessed by PFGE and repetitive-sequence-based PCR (rep-PCR). Phylogenetic grouping was performed among CTX-M-15-producing E. coli.

RESULTS

In total, 73 consecutive non-duplicate ESBL-positive E. coli and 72 K. pneumoniae strains were isolated. The bla(CTX-M-15) gene was found to be highly prevalent (69/73 strains, 94.5%) among the 73 ESBL-positive E. coli isolates. The gene was detected in both clonal and non-clonal isolates, as defined by PFGE and rep-PCR. Sixteen CTX-M-15-positive E. coli could be assigned to a single rep-PCR type and phylogenetic group B2 and belonged to the well-known O25b-ST131 clone. Among the 72 ESBL-positive K. pneumoniae isolates, bla(CTX-M-15) was again the most prevalent ESBL (40/72, 55.6%). Several SHV-type enzymes were also frequently detected: SHV-5 (n = 28); SHV-12 (n = 13); and SHV-2 (n = 6). TEM-type ESBLs were not detected in any of the isolates.

CONCLUSIONS

Indonesia is another developing country affected by the emergence and spread of bacterial strains harbouring ESBL genes, including the CTX-M-15-producing B2-E. coli O25b-ST131 clone.

Authors+Show Affiliations

Department of Medical Microbiology and Infectious Diseases, Erasmus MC, University Medical Center Rotterdam, 's-Gravendijkwal 230, 3015 CE, Rotterdam, The Netherlands. j.severin@erasmusmc.nlNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20053690

Citation

Severin, Juliëtte A., et al. "Molecular Characterization of Extended-spectrum Beta-lactamases in Clinical Escherichia Coli and Klebsiella Pneumoniae Isolates From Surabaya, Indonesia." The Journal of Antimicrobial Chemotherapy, vol. 65, no. 3, 2010, pp. 465-9.
Severin JA, Mertaniasih NM, Kuntaman K, et al. Molecular characterization of extended-spectrum beta-lactamases in clinical Escherichia coli and Klebsiella pneumoniae isolates from Surabaya, Indonesia. J Antimicrob Chemother. 2010;65(3):465-9.
Severin, J. A., Mertaniasih, N. M., Kuntaman, K., Lestari, E. S., Purwanta, M., Lemmens-Den Toom, N., Duerink, D. O., Hadi, U., van Belkum, A., Verbrugh, H. A., & Goessens, W. H. (2010). Molecular characterization of extended-spectrum beta-lactamases in clinical Escherichia coli and Klebsiella pneumoniae isolates from Surabaya, Indonesia. The Journal of Antimicrobial Chemotherapy, 65(3), 465-9. https://doi.org/10.1093/jac/dkp471
Severin JA, et al. Molecular Characterization of Extended-spectrum Beta-lactamases in Clinical Escherichia Coli and Klebsiella Pneumoniae Isolates From Surabaya, Indonesia. J Antimicrob Chemother. 2010;65(3):465-9. PubMed PMID: 20053690.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Molecular characterization of extended-spectrum beta-lactamases in clinical Escherichia coli and Klebsiella pneumoniae isolates from Surabaya, Indonesia. AU - Severin,Juliëtte A, AU - Mertaniasih,Ni Made, AU - Kuntaman,Kuntaman, AU - Lestari,Endang S, AU - Purwanta,Marijam, AU - Lemmens-Den Toom,Nicole, AU - Duerink,D Offra, AU - Hadi,Usman, AU - van Belkum,Alex, AU - Verbrugh,Henri A, AU - Goessens,Wil H, AU - ,, Y1 - 2010/01/06/ PY - 2010/1/8/entrez PY - 2010/1/8/pubmed PY - 2010/4/22/medline SP - 465 EP - 9 JF - The Journal of antimicrobial chemotherapy JO - J Antimicrob Chemother VL - 65 IS - 3 N2 - BACKGROUND: No detailed reports regarding extended-spectrum beta-lactamase (ESBL)-producing Enterobacteriaceae are currently available from Indonesia, the fourth most populous country in the world. METHODS: A survey was carried out to investigate the molecular epidemiology and genetic characteristics of clinical ESBL-producing Escherichia coli and Klebsiella pneumoniae isolates originating from the Dr. Soetomo Academic Hospital in Surabaya, Indonesia, over a 4 month period (January to April 2005). ESBLs were characterized by isoelectric focusing and PCR assays. Clonality of the isolates was assessed by PFGE and repetitive-sequence-based PCR (rep-PCR). Phylogenetic grouping was performed among CTX-M-15-producing E. coli. RESULTS: In total, 73 consecutive non-duplicate ESBL-positive E. coli and 72 K. pneumoniae strains were isolated. The bla(CTX-M-15) gene was found to be highly prevalent (69/73 strains, 94.5%) among the 73 ESBL-positive E. coli isolates. The gene was detected in both clonal and non-clonal isolates, as defined by PFGE and rep-PCR. Sixteen CTX-M-15-positive E. coli could be assigned to a single rep-PCR type and phylogenetic group B2 and belonged to the well-known O25b-ST131 clone. Among the 72 ESBL-positive K. pneumoniae isolates, bla(CTX-M-15) was again the most prevalent ESBL (40/72, 55.6%). Several SHV-type enzymes were also frequently detected: SHV-5 (n = 28); SHV-12 (n = 13); and SHV-2 (n = 6). TEM-type ESBLs were not detected in any of the isolates. CONCLUSIONS: Indonesia is another developing country affected by the emergence and spread of bacterial strains harbouring ESBL genes, including the CTX-M-15-producing B2-E. coli O25b-ST131 clone. SN - 1460-2091 UR - https://www.unboundmedicine.com/medline/citation/20053690/Molecular_characterization_of_extended_spectrum_beta_lactamases_in_clinical_Escherichia_coli_and_Klebsiella_pneumoniae_isolates_from_Surabaya_Indonesia_ L2 - https://academic.oup.com/jac/article-lookup/doi/10.1093/jac/dkp471 DB - PRIME DP - Unbound Medicine ER -