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Effect of probiotic bacterial strains of Lactobacillus, Bifidobacterium, and Enterococcus on enteroaggregative Escherichia coli.
J Infect Chemother. 2010 Feb; 16(1):10-8.JI

Abstract

The effects of nine probiotic strains of Lactobacillus, Bifidobacterium, and Enterococcus on the growth, adhesion activity, and biofilm formation of enteroaggregative Escherichia coli (EAggEC) were examined. The culture supernatant of the E. faecium strain, with or without pH adjustment to a neutral pH, had a strong bactericidal effect on EAggEC, including induction of membrane damage and cell lysis. Supernatants of the L. casei ss. casei and L. casei ss. rhamnosus strains also had a bactericidal effect on EAggEC, but this activity was abolished by pH adjustment to a neutral pH. No inhibitory effect of the culture supernatants of Bifidobacterium or E. faecalis strains was detected. Adhesion of EAggEC to intestinal epithelial cells was not inhibited by the bacterial strains tested. Two strains of L. casei enhanced EAggEC biofilm formation, which was characterized by increased bacterial proliferation. These results suggest that the three different bacterial species; Lactobacillus, Bifidobacterium, and Enterococcus, have different effects on EAggEC, and that further analysis is required for the practical use of these bacteria as probiotics against EAggEC infection.

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Authors+Show Affiliations

Miyazaki Y
Department of Emergency and Critical Care Medicine, Nippon Medical School, Tokyo, Japan.
Kamiya S
No affiliation info available
Hanawa T
No affiliation info available
Fukuda M
No affiliation info available
Kawakami H
No affiliation info available
Takahashi H
No affiliation info available
Yokota H
No affiliation info available

MeSH

AntibiosisBacterial AdhesionBifidobacteriumBiofilmsCaco-2 CellsCell Line, TumorEnterococcusEpithelial CellsEscherichia coliHumansIntestinesLactobacillusProbioticsSpecies Specificity

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20054601

Citation

Miyazaki, Yoshibumi, et al. "Effect of Probiotic Bacterial Strains of Lactobacillus, Bifidobacterium, and Enterococcus On Enteroaggregative Escherichia Coli." Journal of Infection and Chemotherapy : Official Journal of the Japan Society of Chemotherapy, vol. 16, no. 1, 2010, pp. 10-8.
Miyazaki Y, Kamiya S, Hanawa T, et al. Effect of probiotic bacterial strains of Lactobacillus, Bifidobacterium, and Enterococcus on enteroaggregative Escherichia coli. J Infect Chemother. 2010;16(1):10-8.
Miyazaki, Y., Kamiya, S., Hanawa, T., Fukuda, M., Kawakami, H., Takahashi, H., & Yokota, H. (2010). Effect of probiotic bacterial strains of Lactobacillus, Bifidobacterium, and Enterococcus on enteroaggregative Escherichia coli. Journal of Infection and Chemotherapy : Official Journal of the Japan Society of Chemotherapy, 16(1), 10-8. https://doi.org/10.1007/s10156-009-0007-2
Miyazaki Y, et al. Effect of Probiotic Bacterial Strains of Lactobacillus, Bifidobacterium, and Enterococcus On Enteroaggregative Escherichia Coli. J Infect Chemother. 2010;16(1):10-8. PubMed PMID: 20054601.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effect of probiotic bacterial strains of Lactobacillus, Bifidobacterium, and Enterococcus on enteroaggregative Escherichia coli. AU - Miyazaki,Yoshibumi, AU - Kamiya,Shigeru, AU - Hanawa,Tomoko, AU - Fukuda,Minoru, AU - Kawakami,Hayato, AU - Takahashi,Hidemi, AU - Yokota,Hiroyuki, PY - 2009/06/11/received PY - 2009/09/07/accepted PY - 2010/1/8/entrez PY - 2010/1/8/pubmed PY - 2011/7/2/medline SP - 10 EP - 8 JF - Journal of infection and chemotherapy : official journal of the Japan Society of Chemotherapy JO - J Infect Chemother VL - 16 IS - 1 N2 - The effects of nine probiotic strains of Lactobacillus, Bifidobacterium, and Enterococcus on the growth, adhesion activity, and biofilm formation of enteroaggregative Escherichia coli (EAggEC) were examined. The culture supernatant of the E. faecium strain, with or without pH adjustment to a neutral pH, had a strong bactericidal effect on EAggEC, including induction of membrane damage and cell lysis. Supernatants of the L. casei ss. casei and L. casei ss. rhamnosus strains also had a bactericidal effect on EAggEC, but this activity was abolished by pH adjustment to a neutral pH. No inhibitory effect of the culture supernatants of Bifidobacterium or E. faecalis strains was detected. Adhesion of EAggEC to intestinal epithelial cells was not inhibited by the bacterial strains tested. Two strains of L. casei enhanced EAggEC biofilm formation, which was characterized by increased bacterial proliferation. These results suggest that the three different bacterial species; Lactobacillus, Bifidobacterium, and Enterococcus, have different effects on EAggEC, and that further analysis is required for the practical use of these bacteria as probiotics against EAggEC infection. SN - 1437-7780 UR - https://www.unboundmedicine.com/medline/citation/20054601/Effect_of_probiotic_bacterial_strains_of_Lactobacillus_Bifidobacterium_and_Enterococcus_on_enteroaggregative_Escherichia_coli_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1341-321X(10)70626-7 DB - PRIME DP - Unbound Medicine ER -