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Superresolution imaging using single-molecule localization.
Annu Rev Phys Chem 2010; 61:345-67AR

Abstract

Superresolution imaging is a rapidly emerging new field of microscopy that dramatically improves the spatial resolution of light microscopy by over an order of magnitude (approximately 10-20-nm resolution), allowing biological processes to be described at the molecular scale. Here, we discuss a form of superresolution microscopy based on the controlled activation and sampling of sparse subsets of photoconvertible fluorescent molecules. In this single-molecule-based imaging approach, a wide variety of probes have proved valuable, ranging from genetically encodable photoactivatable fluorescent proteins to photoswitchable cyanine dyes. These have been used in diverse applications of superresolution imaging: from three-dimensional, multicolor molecule localization to tracking of nanometric structures and molecules in living cells. Single-molecule-based superresolution imaging thus offers exciting possibilities for obtaining molecular-scale information on biological events occurring at variable timescales.

Authors+Show Affiliations

Biophotonics Section, National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health, Bethesda, Maryland 20892, USA.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Review

Language

eng

PubMed ID

20055680

Citation

Patterson, George, et al. "Superresolution Imaging Using Single-molecule Localization." Annual Review of Physical Chemistry, vol. 61, 2010, pp. 345-67.
Patterson G, Davidson M, Manley S, et al. Superresolution imaging using single-molecule localization. Annu Rev Phys Chem. 2010;61:345-67.
Patterson, G., Davidson, M., Manley, S., & Lippincott-Schwartz, J. (2010). Superresolution imaging using single-molecule localization. Annual Review of Physical Chemistry, 61, pp. 345-67. doi:10.1146/annurev.physchem.012809.103444.
Patterson G, et al. Superresolution Imaging Using Single-molecule Localization. Annu Rev Phys Chem. 2010;61:345-67. PubMed PMID: 20055680.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Superresolution imaging using single-molecule localization. AU - Patterson,George, AU - Davidson,Michael, AU - Manley,Suliana, AU - Lippincott-Schwartz,Jennifer, PY - 2010/1/9/entrez PY - 2010/1/9/pubmed PY - 2010/6/9/medline SP - 345 EP - 67 JF - Annual review of physical chemistry JO - Annu Rev Phys Chem VL - 61 N2 - Superresolution imaging is a rapidly emerging new field of microscopy that dramatically improves the spatial resolution of light microscopy by over an order of magnitude (approximately 10-20-nm resolution), allowing biological processes to be described at the molecular scale. Here, we discuss a form of superresolution microscopy based on the controlled activation and sampling of sparse subsets of photoconvertible fluorescent molecules. In this single-molecule-based imaging approach, a wide variety of probes have proved valuable, ranging from genetically encodable photoactivatable fluorescent proteins to photoswitchable cyanine dyes. These have been used in diverse applications of superresolution imaging: from three-dimensional, multicolor molecule localization to tracking of nanometric structures and molecules in living cells. Single-molecule-based superresolution imaging thus offers exciting possibilities for obtaining molecular-scale information on biological events occurring at variable timescales. SN - 1545-1593 UR - https://www.unboundmedicine.com/medline/citation/20055680/Superresolution_imaging_using_single_molecule_localization_ L2 - http://arjournals.annualreviews.org/doi/full/10.1146/annurev.physchem.012809.103444?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -