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Diallyl trisulphide-induced apoptosis in human melanoma cells involves downregulation of Bcl-2 and Bcl-xL expression and activation of caspases.
Clin Exp Dermatol. 2009 Dec; 34(8):e537-43.CE

Abstract

BACKGROUND

Although diallyl trisulphide (DATS) has been found to induce apoptosis in various tumour cells, its cytotoxicity in melanoma cells has not yet been defined and the molecular pathway by which DATS induces apoptosis is not well understood.

OBJECTIVES

To determine growth inhibition of DATS in human melanoma cells (A375 and M14) by inducing apoptosis, and to investigate the mechanism underlying such effects.

METHODS

Growth inhibition by DATS was estimated by the tetrazolium assay. Apoptosis induction in DATS-treated cells was assessed by staining with 4',6-diamidino-2-phenylindole (DAPI) and double staining with annexin V and propidium iodide. Expression of Bcl-2, Bax, Bcl-xL/Bcl-xS, cytochrome c release, activation of caspase-9 and poly(ADP-ribose) polymerase (PARP) were determined by western blotting. The activity of caspase-3 was measured using a colorimetric assay.

RESULTS

DATS exerted its cytotoxic effect in a time-dependent and dose-dependent manner by inducing apoptosis in A375 and M14 cells. Expression of Bcl-2 and Bcl-xL was downregulated. Release of cytochrome c and activation of the downstream effectors caspase-3, caspase-9 and PARP were detected after DATS sensitization.

CONCLUSIONS

DATS inhibits growth of melanoma cells by inducing apoptosis in association with downregulation of Bcl-2 and Bcl-xL and activation of caspases.

Authors+Show Affiliations

Department of Dermatology, Second Affiliated Hospital, Sun Yat-Sen University, Guangdong, China.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

20055834

Citation

Zhou, C, et al. "Diallyl Trisulphide-induced Apoptosis in Human Melanoma Cells Involves Downregulation of Bcl-2 and Bcl-xL Expression and Activation of Caspases." Clinical and Experimental Dermatology, vol. 34, no. 8, 2009, pp. e537-43.
Zhou C, Mao XP, Guo Q, et al. Diallyl trisulphide-induced apoptosis in human melanoma cells involves downregulation of Bcl-2 and Bcl-xL expression and activation of caspases. Clin Exp Dermatol. 2009;34(8):e537-43.
Zhou, C., Mao, X. P., Guo, Q., & Zeng, F. Q. (2009). Diallyl trisulphide-induced apoptosis in human melanoma cells involves downregulation of Bcl-2 and Bcl-xL expression and activation of caspases. Clinical and Experimental Dermatology, 34(8), e537-43. https://doi.org/10.1111/j.1365-2230.2009.03594.x
Zhou C, et al. Diallyl Trisulphide-induced Apoptosis in Human Melanoma Cells Involves Downregulation of Bcl-2 and Bcl-xL Expression and Activation of Caspases. Clin Exp Dermatol. 2009;34(8):e537-43. PubMed PMID: 20055834.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Diallyl trisulphide-induced apoptosis in human melanoma cells involves downregulation of Bcl-2 and Bcl-xL expression and activation of caspases. AU - Zhou,C, AU - Mao,X-P, AU - Guo,Q, AU - Zeng,F-Q, PY - 2010/1/9/entrez PY - 2010/1/9/pubmed PY - 2010/8/19/medline SP - e537 EP - 43 JF - Clinical and experimental dermatology JO - Clin. Exp. Dermatol. VL - 34 IS - 8 N2 - BACKGROUND: Although diallyl trisulphide (DATS) has been found to induce apoptosis in various tumour cells, its cytotoxicity in melanoma cells has not yet been defined and the molecular pathway by which DATS induces apoptosis is not well understood. OBJECTIVES: To determine growth inhibition of DATS in human melanoma cells (A375 and M14) by inducing apoptosis, and to investigate the mechanism underlying such effects. METHODS: Growth inhibition by DATS was estimated by the tetrazolium assay. Apoptosis induction in DATS-treated cells was assessed by staining with 4',6-diamidino-2-phenylindole (DAPI) and double staining with annexin V and propidium iodide. Expression of Bcl-2, Bax, Bcl-xL/Bcl-xS, cytochrome c release, activation of caspase-9 and poly(ADP-ribose) polymerase (PARP) were determined by western blotting. The activity of caspase-3 was measured using a colorimetric assay. RESULTS: DATS exerted its cytotoxic effect in a time-dependent and dose-dependent manner by inducing apoptosis in A375 and M14 cells. Expression of Bcl-2 and Bcl-xL was downregulated. Release of cytochrome c and activation of the downstream effectors caspase-3, caspase-9 and PARP were detected after DATS sensitization. CONCLUSIONS: DATS inhibits growth of melanoma cells by inducing apoptosis in association with downregulation of Bcl-2 and Bcl-xL and activation of caspases. SN - 1365-2230 UR - https://www.unboundmedicine.com/medline/citation/20055834/Diallyl_trisulphide_induced_apoptosis_in_human_melanoma_cells_involves_downregulation_of_Bcl_2_and_Bcl_xL_expression_and_activation_of_caspases_ L2 - https://doi.org/10.1111/j.1365-2230.2009.03594.x DB - PRIME DP - Unbound Medicine ER -