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Epithelial-mesenchymal transition in human lungs with usual interstitial pneumonia: quantitative immunohistochemistry.
Pathol Int. 2010 Jan; 60(1):14-21.PI

Abstract

Fibroblastic foci, a major histological feature of usual interstitial pneumonia (UIP), play a critical role in the development of UIP. The mechanisms involved in the formation of these foci, however, including cellular origin, remain unclear. Recent in vitro and animal studies suggested epithelial-mesenchymal transition (EMT) of alveolar epithelial cells during pulmonary fibrogenesis. The aim of the present study was to investigate the presence of EMT in patients with UIP on quantitative immunohistochemistry using pathological tissue sections. The study subjects were 13 patients with UIP pattern among 52 patients with interstitial pneumonia who underwent lung biopsy. Alveolar epithelial cells overlying fibroblastic foci expressed epithelial markers less frequently and mesenchymal markers more frequently compared with those in non-diseased control lung tissues (n= 10). Moreover, double immunostaining showed that some epithelial cells stained for both epithelial and mesenchymal markers. Furthermore, significantly higher numbers of epithelial marker-positive fibroblastic cells were found in fibroblastic foci in UIP as well as in other non-UIP fibrosing diseases than in control lung tissues. The results showed that some epithelial cells overlying fibroblastic foci lose the epithelial phenotype and gain the mesenchymal phenotype, and that some fibroblastic cells in fibroblastic foci originate from epithelial cells. But this EMT may not be specific for UIP.

Authors+Show Affiliations

Department of Pathology, Fukuoka University Hospital and School of Medicine, Fukuoka 814-0180, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

20055947

Citation

Harada, Taishi, et al. "Epithelial-mesenchymal Transition in Human Lungs With Usual Interstitial Pneumonia: Quantitative Immunohistochemistry." Pathology International, vol. 60, no. 1, 2010, pp. 14-21.
Harada T, Nabeshima K, Hamasaki M, et al. Epithelial-mesenchymal transition in human lungs with usual interstitial pneumonia: quantitative immunohistochemistry. Pathol Int. 2010;60(1):14-21.
Harada, T., Nabeshima, K., Hamasaki, M., Uesugi, N., Watanabe, K., & Iwasaki, H. (2010). Epithelial-mesenchymal transition in human lungs with usual interstitial pneumonia: quantitative immunohistochemistry. Pathology International, 60(1), 14-21. https://doi.org/10.1111/j.1440-1827.2009.02469.x
Harada T, et al. Epithelial-mesenchymal Transition in Human Lungs With Usual Interstitial Pneumonia: Quantitative Immunohistochemistry. Pathol Int. 2010;60(1):14-21. PubMed PMID: 20055947.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Epithelial-mesenchymal transition in human lungs with usual interstitial pneumonia: quantitative immunohistochemistry. AU - Harada,Taishi, AU - Nabeshima,Kazuki, AU - Hamasaki,Makoto, AU - Uesugi,Noriko, AU - Watanabe,Kentaro, AU - Iwasaki,Hiroshi, PY - 2010/1/9/entrez PY - 2010/1/9/pubmed PY - 2010/4/1/medline SP - 14 EP - 21 JF - Pathology international JO - Pathol Int VL - 60 IS - 1 N2 - Fibroblastic foci, a major histological feature of usual interstitial pneumonia (UIP), play a critical role in the development of UIP. The mechanisms involved in the formation of these foci, however, including cellular origin, remain unclear. Recent in vitro and animal studies suggested epithelial-mesenchymal transition (EMT) of alveolar epithelial cells during pulmonary fibrogenesis. The aim of the present study was to investigate the presence of EMT in patients with UIP on quantitative immunohistochemistry using pathological tissue sections. The study subjects were 13 patients with UIP pattern among 52 patients with interstitial pneumonia who underwent lung biopsy. Alveolar epithelial cells overlying fibroblastic foci expressed epithelial markers less frequently and mesenchymal markers more frequently compared with those in non-diseased control lung tissues (n= 10). Moreover, double immunostaining showed that some epithelial cells stained for both epithelial and mesenchymal markers. Furthermore, significantly higher numbers of epithelial marker-positive fibroblastic cells were found in fibroblastic foci in UIP as well as in other non-UIP fibrosing diseases than in control lung tissues. The results showed that some epithelial cells overlying fibroblastic foci lose the epithelial phenotype and gain the mesenchymal phenotype, and that some fibroblastic cells in fibroblastic foci originate from epithelial cells. But this EMT may not be specific for UIP. SN - 1440-1827 UR - https://www.unboundmedicine.com/medline/citation/20055947/Epithelial_mesenchymal_transition_in_human_lungs_with_usual_interstitial_pneumonia:_quantitative_immunohistochemistry_ L2 - https://doi.org/10.1111/j.1440-1827.2009.02469.x DB - PRIME DP - Unbound Medicine ER -