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Phenolic substance characterization and chemical and cell-based antioxidant activities of 11 lentils grown in the northern United States.
J Agric Food Chem. 2010 Feb 10; 58(3):1509-17.JA

Abstract

Chemical and cellular antioxidant activities and phenolic profiles of 11 lentil cultivars grown in the cool northern parts of the United States were investigated. Individual phenolic compounds, including phenolic acids, flavan-3-ols, flavones, and anthocyanins, were further quantitatively investigated by HPLC. Cellular antioxidant activities (CAA) and peroxyl radical scavenging capacity (PRSC) were evaluated by fluorescence microplate reader. Cultivar Morton exhibited the highest individual flavan-3-ols (catechin and epicatechin) and total flavonoids, as well as the highest antioxidant properties (PRSC and CAA) among all lentils tested. Five phenolic acids of the benzoic types and their derivates (gallic, protocatechuic, 2,3,4-trihydroxybenzoic, p-hydroxybenzoic acid, and protocatechualdehyde) and four phenolic acids of the cinnamic type (chlorogenic, p-coumaric, m-coumaric, and sinapic acid) were detected in all lentil cultivars. Two flavan-3-ols [(+)-catechin and (-)-epicatechin] and one flavone (luteolin) were detected in all lentil cultivars. Among all phenolic compounds detected, sinapic acid was the predominant phenolic acid, and (+)-catechin and (-)-epicatechin were the predominant flavonoids. These results showed that different phenotype lentils possessed considerable variations in their individual phenolic compounds, as well as chemical and cellular antioxidant activities. Caffeic acid, catechin, epicatechin, and total flavonoids significantly (p < 0.05) correlated with peroxyl radical scavenging assay. Cellular antioxidant assay significantly correlated with chemical antioxidant assay ORAC. The results from this study could be very interesting for breeding programs to improve lentils for use as functional foods.

Authors+Show Affiliations

Food Science and Technology Program, Beijing Normal University-Hong Kong Baptist University United International College, Zhuhai, Guangdong 519085, China.No affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20058926

Citation

Xu, Baojun, and Sam K C. Chang. "Phenolic Substance Characterization and Chemical and Cell-based Antioxidant Activities of 11 Lentils Grown in the Northern United States." Journal of Agricultural and Food Chemistry, vol. 58, no. 3, 2010, pp. 1509-17.
Xu B, Chang SK. Phenolic substance characterization and chemical and cell-based antioxidant activities of 11 lentils grown in the northern United States. J Agric Food Chem. 2010;58(3):1509-17.
Xu, B., & Chang, S. K. (2010). Phenolic substance characterization and chemical and cell-based antioxidant activities of 11 lentils grown in the northern United States. Journal of Agricultural and Food Chemistry, 58(3), 1509-17. https://doi.org/10.1021/jf903532y
Xu B, Chang SK. Phenolic Substance Characterization and Chemical and Cell-based Antioxidant Activities of 11 Lentils Grown in the Northern United States. J Agric Food Chem. 2010 Feb 10;58(3):1509-17. PubMed PMID: 20058926.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Phenolic substance characterization and chemical and cell-based antioxidant activities of 11 lentils grown in the northern United States. AU - Xu,Baojun, AU - Chang,Sam K C, PY - 2010/1/12/entrez PY - 2010/1/12/pubmed PY - 2010/4/20/medline SP - 1509 EP - 17 JF - Journal of agricultural and food chemistry JO - J Agric Food Chem VL - 58 IS - 3 N2 - Chemical and cellular antioxidant activities and phenolic profiles of 11 lentil cultivars grown in the cool northern parts of the United States were investigated. Individual phenolic compounds, including phenolic acids, flavan-3-ols, flavones, and anthocyanins, were further quantitatively investigated by HPLC. Cellular antioxidant activities (CAA) and peroxyl radical scavenging capacity (PRSC) were evaluated by fluorescence microplate reader. Cultivar Morton exhibited the highest individual flavan-3-ols (catechin and epicatechin) and total flavonoids, as well as the highest antioxidant properties (PRSC and CAA) among all lentils tested. Five phenolic acids of the benzoic types and their derivates (gallic, protocatechuic, 2,3,4-trihydroxybenzoic, p-hydroxybenzoic acid, and protocatechualdehyde) and four phenolic acids of the cinnamic type (chlorogenic, p-coumaric, m-coumaric, and sinapic acid) were detected in all lentil cultivars. Two flavan-3-ols [(+)-catechin and (-)-epicatechin] and one flavone (luteolin) were detected in all lentil cultivars. Among all phenolic compounds detected, sinapic acid was the predominant phenolic acid, and (+)-catechin and (-)-epicatechin were the predominant flavonoids. These results showed that different phenotype lentils possessed considerable variations in their individual phenolic compounds, as well as chemical and cellular antioxidant activities. Caffeic acid, catechin, epicatechin, and total flavonoids significantly (p < 0.05) correlated with peroxyl radical scavenging assay. Cellular antioxidant assay significantly correlated with chemical antioxidant assay ORAC. The results from this study could be very interesting for breeding programs to improve lentils for use as functional foods. SN - 1520-5118 UR - https://www.unboundmedicine.com/medline/citation/20058926/Phenolic_substance_characterization_and_chemical_and_cell_based_antioxidant_activities_of_11_lentils_grown_in_the_northern_United_States_ L2 - https://doi.org/10.1021/jf903532y DB - PRIME DP - Unbound Medicine ER -