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Rapid and simple determination of psychotropic phenylalkylamine derivatives in human hair by gas chromatography-mass spectrometry using micro-pulverized extraction.
Forensic Sci Int. 2010 Mar 20; 196(1-3):43-50.FS

Abstract

A gas chromatography-mass spectrometric (GC-MS) method was developed and validated for the determination of five psychotropic phenylalkylamine derivatives (amphetamine, AP; methamphetamine, MA; 3,4-methylenedioxyamphetamine, MDA; 3,4-methylenedioxymethamphetamine, MDMA; norketamine, NKT) in human hair. Hair samples (10mg) were washed with distilled water and acetone, mechanically pulverized for 1.5 min with a bead mill, and then incubated in 1 mL of methanol under ultrasonication at 50 degrees C for 1h. The resulting solutions were evaporated to dryness, derivatized using heptafluorobutyric anhydride (HFBA) at 50 degrees C for 30 min, and analyzed by GC-MS. The linear ranges were 0.1-20.0 ng/mg for AP and MA and 0.05-20.0 ng/mg for MDA, MDMA, and NKT, with the coefficients of determination (r(2)>0.9982). The intra-day and inter-day precisions were within 11.5% and 12.8%, respectively. The intra-day and inter-day accuracies were -4.1% to 5.8% and -6.6% to 4.2%, respectively. The limits of detections (LODs) for each compound were lower than 0.028 ng/mg. The recoveries were in the range of 78.9-101.2%. Based on these results, the method proved to be effective for the rapid and simple determination of phenylalkylamine derivatives in hair specimens.

Authors+Show Affiliations

Drug Analysis Laboratory, Forensic Science Division, Supreme Prosecutors' Office, Seoul, Republic of Korea. paxus@spo.go.krNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Validation Study

Language

eng

PubMed ID

20060242

Citation

Kim, Jin Young, et al. "Rapid and Simple Determination of Psychotropic Phenylalkylamine Derivatives in Human Hair By Gas Chromatography-mass Spectrometry Using Micro-pulverized Extraction." Forensic Science International, vol. 196, no. 1-3, 2010, pp. 43-50.
Kim JY, Shin SH, Lee JI, et al. Rapid and simple determination of psychotropic phenylalkylamine derivatives in human hair by gas chromatography-mass spectrometry using micro-pulverized extraction. Forensic Sci Int. 2010;196(1-3):43-50.
Kim, J. Y., Shin, S. H., Lee, J. I., & In, M. K. (2010). Rapid and simple determination of psychotropic phenylalkylamine derivatives in human hair by gas chromatography-mass spectrometry using micro-pulverized extraction. Forensic Science International, 196(1-3), 43-50. https://doi.org/10.1016/j.forsciint.2009.12.045
Kim JY, et al. Rapid and Simple Determination of Psychotropic Phenylalkylamine Derivatives in Human Hair By Gas Chromatography-mass Spectrometry Using Micro-pulverized Extraction. Forensic Sci Int. 2010 Mar 20;196(1-3):43-50. PubMed PMID: 20060242.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Rapid and simple determination of psychotropic phenylalkylamine derivatives in human hair by gas chromatography-mass spectrometry using micro-pulverized extraction. AU - Kim,Jin Young, AU - Shin,Soon Ho, AU - Lee,Jae Il, AU - In,Moon Kyo, Y1 - 2010/01/13/ PY - 2009/06/05/received PY - 2009/07/25/accepted PY - 2010/1/12/entrez PY - 2010/1/12/pubmed PY - 2010/6/11/medline SP - 43 EP - 50 JF - Forensic science international JO - Forensic Sci Int VL - 196 IS - 1-3 N2 - A gas chromatography-mass spectrometric (GC-MS) method was developed and validated for the determination of five psychotropic phenylalkylamine derivatives (amphetamine, AP; methamphetamine, MA; 3,4-methylenedioxyamphetamine, MDA; 3,4-methylenedioxymethamphetamine, MDMA; norketamine, NKT) in human hair. Hair samples (10mg) were washed with distilled water and acetone, mechanically pulverized for 1.5 min with a bead mill, and then incubated in 1 mL of methanol under ultrasonication at 50 degrees C for 1h. The resulting solutions were evaporated to dryness, derivatized using heptafluorobutyric anhydride (HFBA) at 50 degrees C for 30 min, and analyzed by GC-MS. The linear ranges were 0.1-20.0 ng/mg for AP and MA and 0.05-20.0 ng/mg for MDA, MDMA, and NKT, with the coefficients of determination (r(2)>0.9982). The intra-day and inter-day precisions were within 11.5% and 12.8%, respectively. The intra-day and inter-day accuracies were -4.1% to 5.8% and -6.6% to 4.2%, respectively. The limits of detections (LODs) for each compound were lower than 0.028 ng/mg. The recoveries were in the range of 78.9-101.2%. Based on these results, the method proved to be effective for the rapid and simple determination of phenylalkylamine derivatives in hair specimens. SN - 1872-6283 UR - https://www.unboundmedicine.com/medline/citation/20060242/Rapid_and_simple_determination_of_psychotropic_phenylalkylamine_derivatives_in_human_hair_by_gas_chromatography_mass_spectrometry_using_micro_pulverized_extraction_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0379-0738(09)00546-5 DB - PRIME DP - Unbound Medicine ER -