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An IL-15 adjuvant enhances the efficacy of a combined DNA vaccine against Brucella by increasing the CD8+ cytotoxic T cell response.
Vaccine. 2010 Mar 11; 28(12):2408-15.V

Abstract

We investigated whether a combined DNA vaccine delivered together with the IL-15 gene (DNA-IL-15(+)) enhanced the immune response against Brucella abortus in mice. Mice vaccinated with DNA-IL-15(+) developed a robust humoral response; Brucella-specific antibodies exhibited a dominance of immunoglobulin G2a (IgG2a) over IgG1. Splenocytes from DNA-IL-15(+)-vaccinated mice induced significantly higher levels of IFN-gamma (P<0.01) and CD8(+) T cell response (P<0.01), suggesting induction of a T-helper-1-dominated immune response. In a specific cytotoxic-T-lymphocyte activity assay, DNA-IL-15(+) immunization elicited mainly CD8(+) T cells, which mediate cytotoxicity, but also CD4(+) T cells. In vivo depletion of T cell subsets showed that the DNA-IL-15(+)-induced protection against Brucella infection is mediated predominantly by CD8(+) T cells, although CD4(+) T cells also contribute. These data indicate that plasmid-delivered IL-15 increases the efficacy of the Brucella DNA vaccine.

Authors+Show Affiliations

The Institute of Veterinary Medicine, Xinjiang Academy of Animal Science, Urumqi 830000, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20064480

Citation

Hu, Xi-Dan, et al. "An IL-15 Adjuvant Enhances the Efficacy of a Combined DNA Vaccine Against Brucella By Increasing the CD8+ Cytotoxic T Cell Response." Vaccine, vol. 28, no. 12, 2010, pp. 2408-15.
Hu XD, Chen ST, Li JY, et al. An IL-15 adjuvant enhances the efficacy of a combined DNA vaccine against Brucella by increasing the CD8+ cytotoxic T cell response. Vaccine. 2010;28(12):2408-15.
Hu, X. D., Chen, S. T., Li, J. Y., Yu, D. H., Yi-Zhang, ., & Cai, H. (2010). An IL-15 adjuvant enhances the efficacy of a combined DNA vaccine against Brucella by increasing the CD8+ cytotoxic T cell response. Vaccine, 28(12), 2408-15. https://doi.org/10.1016/j.vaccine.2009.12.076
Hu XD, et al. An IL-15 Adjuvant Enhances the Efficacy of a Combined DNA Vaccine Against Brucella By Increasing the CD8+ Cytotoxic T Cell Response. Vaccine. 2010 Mar 11;28(12):2408-15. PubMed PMID: 20064480.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - An IL-15 adjuvant enhances the efficacy of a combined DNA vaccine against Brucella by increasing the CD8+ cytotoxic T cell response. AU - Hu,Xi-Dan, AU - Chen,Su-Ting, AU - Li,Jia-Yun, AU - Yu,Da-Hai, AU - Yi-Zhang,, AU - Cai,Hong, Y1 - 2010/01/09/ PY - 2009/10/23/received PY - 2009/12/18/revised PY - 2009/12/27/accepted PY - 2010/1/13/entrez PY - 2010/1/13/pubmed PY - 2010/5/21/medline SP - 2408 EP - 15 JF - Vaccine JO - Vaccine VL - 28 IS - 12 N2 - We investigated whether a combined DNA vaccine delivered together with the IL-15 gene (DNA-IL-15(+)) enhanced the immune response against Brucella abortus in mice. Mice vaccinated with DNA-IL-15(+) developed a robust humoral response; Brucella-specific antibodies exhibited a dominance of immunoglobulin G2a (IgG2a) over IgG1. Splenocytes from DNA-IL-15(+)-vaccinated mice induced significantly higher levels of IFN-gamma (P<0.01) and CD8(+) T cell response (P<0.01), suggesting induction of a T-helper-1-dominated immune response. In a specific cytotoxic-T-lymphocyte activity assay, DNA-IL-15(+) immunization elicited mainly CD8(+) T cells, which mediate cytotoxicity, but also CD4(+) T cells. In vivo depletion of T cell subsets showed that the DNA-IL-15(+)-induced protection against Brucella infection is mediated predominantly by CD8(+) T cells, although CD4(+) T cells also contribute. These data indicate that plasmid-delivered IL-15 increases the efficacy of the Brucella DNA vaccine. SN - 1873-2518 UR - https://www.unboundmedicine.com/medline/citation/20064480/An_IL_15_adjuvant_enhances_the_efficacy_of_a_combined_DNA_vaccine_against_Brucella_by_increasing_the_CD8+_cytotoxic_T_cell_response_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0264-410X(10)00012-5 DB - PRIME DP - Unbound Medicine ER -