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In vitro antioxidant and in vivo prophylactic effects of two gamma-lactones isolated from Grewia tiliaefolia against hepatotoxicity in carbon tetrachloride intoxicated rats.
Eur J Pharmacol. 2010 Apr 10; 631(1-3):42-52.EJ

Abstract

Grewia tiliaefolia is widely used in traditional Indian medicines to cure jaundice, biliousness, dysentery and the diseases of blood. Bioassay-guided fractionation of methanolic extract of the G. tiliaefolia bark has resulted in the isolation of D-erythro-2-hexenoic acid gamma-lactone (EHGL) and gulonic acid gamma-lactone (GAGL). Hepatoprotective activity of the methanolic extract and the isolated constituents were evaluated against CCl(4)-induced hepatotoxicity in rats. The treatment with methanolic extract, EHGL and GAGL at oral doses of 100, 150 and 60 mg/kg respectively with concomitant CCl(4) intraperitoneal injection (1 ml/kg) significantly reduced the elevated plasma levels of aminotransferases, alkaline phosphatase and the incidence of liver necrosis compared with the CCl(4)-injected group without affecting the concentrations of serum bilirubin and hepatic markers. EHGL and GAGL significantly inhibited the elevated levels of thiobarbituric acid reactive substances and glutathione in liver homogenates. Histology of the liver tissues of the extract and isolated constituents treated groups showed the presence of normal hepatic cords, absence of necrosis and fatty infiltration as similar to the normal control. The results revealed that the hepatoprotective activity of EHGL is significant as similar to the standard drug silymarin. To clarify the influence of the extract and isolated constituents on the protection of oxidative-hepatic damage, we examined in vitro antioxidant properties of the test compounds. The extract and the constituents showed significant free radical scavenging activity. These results suggest that the extract as well as the constituents could protect the hepatocytes from CCl(4)-induced liver damage perhaps, by their anti-oxidative effect on hepatocytes, hence eliminating the deleterious effects of toxic metabolites from CCl(4).

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Publisher Full Text (DOI)

Authors+Show Affiliations

Khadeer Ahamed MB
Department of Genetics, School of Ecology and Conservation Genetics, GKVK Campus, University of Agricultural Science, Bangalore-560 065, Karnataka, India.
Krishna V
No affiliation info available
Dandin CJ
No affiliation info available

MeSH

AnimalsAntioxidantsBiomarkersCaproatesCarbon TetrachlorideChemical and Drug Induced Liver InjuryDose-Response Relationship, DrugFemaleGluconatesGrewiaLactonesLethal Dose 50Lipid PeroxidationMaleMedicine, TraditionalMolecular StructureOxidative StressPhytotherapyPlant BarkPlant ExtractsPlant StemsProtective AgentsRatsRats, Wistar

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

20064503

Citation

Khadeer Ahamed, Mohamed B., et al. "In Vitro Antioxidant and in Vivo Prophylactic Effects of Two Gamma-lactones Isolated From Grewia Tiliaefolia Against Hepatotoxicity in Carbon Tetrachloride Intoxicated Rats." European Journal of Pharmacology, vol. 631, no. 1-3, 2010, pp. 42-52.
Khadeer Ahamed MB, Krishna V, Dandin CJ. In vitro antioxidant and in vivo prophylactic effects of two gamma-lactones isolated from Grewia tiliaefolia against hepatotoxicity in carbon tetrachloride intoxicated rats. Eur J Pharmacol. 2010;631(1-3):42-52.
Khadeer Ahamed, M. B., Krishna, V., & Dandin, C. J. (2010). In vitro antioxidant and in vivo prophylactic effects of two gamma-lactones isolated from Grewia tiliaefolia against hepatotoxicity in carbon tetrachloride intoxicated rats. European Journal of Pharmacology, 631(1-3), 42-52. https://doi.org/10.1016/j.ejphar.2009.12.034
Khadeer Ahamed MB, Krishna V, Dandin CJ. In Vitro Antioxidant and in Vivo Prophylactic Effects of Two Gamma-lactones Isolated From Grewia Tiliaefolia Against Hepatotoxicity in Carbon Tetrachloride Intoxicated Rats. Eur J Pharmacol. 2010 Apr 10;631(1-3):42-52. PubMed PMID: 20064503.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - In vitro antioxidant and in vivo prophylactic effects of two gamma-lactones isolated from Grewia tiliaefolia against hepatotoxicity in carbon tetrachloride intoxicated rats. AU - Khadeer Ahamed,Mohamed B, AU - Krishna,Venkatarangaiah, AU - Dandin,Chethan J, Y1 - 2010/01/11/ PY - 2009/09/26/received PY - 2009/11/23/revised PY - 2009/12/15/accepted PY - 2010/1/13/entrez PY - 2010/1/13/pubmed PY - 2010/5/18/medline SP - 42 EP - 52 JF - European journal of pharmacology JO - Eur J Pharmacol VL - 631 IS - 1-3 N2 - Grewia tiliaefolia is widely used in traditional Indian medicines to cure jaundice, biliousness, dysentery and the diseases of blood. Bioassay-guided fractionation of methanolic extract of the G. tiliaefolia bark has resulted in the isolation of D-erythro-2-hexenoic acid gamma-lactone (EHGL) and gulonic acid gamma-lactone (GAGL). Hepatoprotective activity of the methanolic extract and the isolated constituents were evaluated against CCl(4)-induced hepatotoxicity in rats. The treatment with methanolic extract, EHGL and GAGL at oral doses of 100, 150 and 60 mg/kg respectively with concomitant CCl(4) intraperitoneal injection (1 ml/kg) significantly reduced the elevated plasma levels of aminotransferases, alkaline phosphatase and the incidence of liver necrosis compared with the CCl(4)-injected group without affecting the concentrations of serum bilirubin and hepatic markers. EHGL and GAGL significantly inhibited the elevated levels of thiobarbituric acid reactive substances and glutathione in liver homogenates. Histology of the liver tissues of the extract and isolated constituents treated groups showed the presence of normal hepatic cords, absence of necrosis and fatty infiltration as similar to the normal control. The results revealed that the hepatoprotective activity of EHGL is significant as similar to the standard drug silymarin. To clarify the influence of the extract and isolated constituents on the protection of oxidative-hepatic damage, we examined in vitro antioxidant properties of the test compounds. The extract and the constituents showed significant free radical scavenging activity. These results suggest that the extract as well as the constituents could protect the hepatocytes from CCl(4)-induced liver damage perhaps, by their anti-oxidative effect on hepatocytes, hence eliminating the deleterious effects of toxic metabolites from CCl(4). SN - 1879-0712 UR - https://www.unboundmedicine.com/medline/citation/20064503/In_vitro_antioxidant_and_in_vivo_prophylactic_effects_of_two_gamma_lactones_isolated_from_Grewia_tiliaefolia_against_hepatotoxicity_in_carbon_tetrachloride_intoxicated_rats_ DB - PRIME DP - Unbound Medicine ER -