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Electrochemistry and electrocatalytic of hemoglobin immobilized on FDU-15-Pt mesoporous materials.
Bioelectrochemistry. 2010 Oct; 79(2):248-53.B

Abstract

In this paper, the composite films of Nafion/Hb/FDU-Pt-15/PDDA were constructed by layer-by-layer assembly technique. The FDU-15-Pt mesoporous materials were synthesized and characterized by transmission electron microscopy (TEM) and X-ray diffraction (XRD). The resulting films were verified by electrochemical impedance spectroscopy (EIS), cyclic voltammetry and UV-vis spectroscopy. The direct electrochemical and electrocatalytic properties of hemoglobin (Hb) in the Nafion/Hb/FDU-15-Pt/PDDA films were also investigated. A pair of stable and well-defined reversible redox peaks was observed and the formal potential of Hb Fe(III)/Fe(II) redox couple was found to be -0.324 V (vs. SCE). Based on a series of optimized conditions, the films modified electrode displayed a good electrocatalytic activity to the reduction of hydrogen peroxide (H2O2), which had linear current response from 2.0 x 10(-6) M to 6.0 x 10(-2) M with the detection limit of 1.0 x 10(-6) M (S/N=3). The apparent Michaelis-Menten constant (K(m)(app)) was determined to be 0.15 mM in the range of lower concentration of H2O2. The apparent heterogeneous electron transfer rate constant (k(s)) was 1.05 +/- 0.03 s(-1). The good sensitivity, reproducibility, excellent stability and wide linear range make Nafion/Hb/FDU-15-Pt/PDDA a promising application of the preparation of third-generation biosensor.

Authors+Show Affiliations

Department of Chemistry and Shanghai Key Laboratory of Green Chemistry and Chemical Process, East China Normal University, 3663 Zhongshan Road(N), Shanghai 200062, PR China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20064750

Citation

Nie, Dongxia, et al. "Electrochemistry and Electrocatalytic of Hemoglobin Immobilized On FDU-15-Pt Mesoporous Materials." Bioelectrochemistry (Amsterdam, Netherlands), vol. 79, no. 2, 2010, pp. 248-53.
Nie D, Liang Y, Zhou T, et al. Electrochemistry and electrocatalytic of hemoglobin immobilized on FDU-15-Pt mesoporous materials. Bioelectrochemistry. 2010;79(2):248-53.
Nie, D., Liang, Y., Zhou, T., Li, X., Shi, G., & Jin, L. (2010). Electrochemistry and electrocatalytic of hemoglobin immobilized on FDU-15-Pt mesoporous materials. Bioelectrochemistry (Amsterdam, Netherlands), 79(2), 248-53. https://doi.org/10.1016/j.bioelechem.2009.12.008
Nie D, et al. Electrochemistry and Electrocatalytic of Hemoglobin Immobilized On FDU-15-Pt Mesoporous Materials. Bioelectrochemistry. 2010;79(2):248-53. PubMed PMID: 20064750.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Electrochemistry and electrocatalytic of hemoglobin immobilized on FDU-15-Pt mesoporous materials. AU - Nie,Dongxia, AU - Liang,Ying, AU - Zhou,Tianshu, AU - Li,Xiaohong, AU - Shi,Guoyue, AU - Jin,Litong, Y1 - 2009/12/28/ PY - 2009/07/13/received PY - 2009/11/24/revised PY - 2009/12/08/accepted PY - 2010/1/13/entrez PY - 2010/1/13/pubmed PY - 2010/12/18/medline SP - 248 EP - 53 JF - Bioelectrochemistry (Amsterdam, Netherlands) JO - Bioelectrochemistry VL - 79 IS - 2 N2 - In this paper, the composite films of Nafion/Hb/FDU-Pt-15/PDDA were constructed by layer-by-layer assembly technique. The FDU-15-Pt mesoporous materials were synthesized and characterized by transmission electron microscopy (TEM) and X-ray diffraction (XRD). The resulting films were verified by electrochemical impedance spectroscopy (EIS), cyclic voltammetry and UV-vis spectroscopy. The direct electrochemical and electrocatalytic properties of hemoglobin (Hb) in the Nafion/Hb/FDU-15-Pt/PDDA films were also investigated. A pair of stable and well-defined reversible redox peaks was observed and the formal potential of Hb Fe(III)/Fe(II) redox couple was found to be -0.324 V (vs. SCE). Based on a series of optimized conditions, the films modified electrode displayed a good electrocatalytic activity to the reduction of hydrogen peroxide (H2O2), which had linear current response from 2.0 x 10(-6) M to 6.0 x 10(-2) M with the detection limit of 1.0 x 10(-6) M (S/N=3). The apparent Michaelis-Menten constant (K(m)(app)) was determined to be 0.15 mM in the range of lower concentration of H2O2. The apparent heterogeneous electron transfer rate constant (k(s)) was 1.05 +/- 0.03 s(-1). The good sensitivity, reproducibility, excellent stability and wide linear range make Nafion/Hb/FDU-15-Pt/PDDA a promising application of the preparation of third-generation biosensor. SN - 1878-562X UR - https://www.unboundmedicine.com/medline/citation/20064750/Electrochemistry_and_electrocatalytic_of_hemoglobin_immobilized_on_FDU_15_Pt_mesoporous_materials_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1567-5394(09)00224-2 DB - PRIME DP - Unbound Medicine ER -