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Thermal contribution to the inactivation of Cryptosporidium in plastic bottles during solar water disinfection procedures.
Am J Trop Med Hyg. 2010 Jan; 82(1):35-9.AJ

Abstract

To determine the thermal contribution, independent of ultraviolet radiation, on the inactivation of Cryptosporidium parvum during solar water disinfection procedures (SODIS), oocysts were exposed for 4, 8, and 12 hours to temperatures recorded in polyethylene terephthalate bottles in previous SODIS studies carried out under field conditions. Inclusion/exclusion of the fluorogenic vital dye propidium iodide, spontaneous excystation, and infectivity studies were used to determine the inactivation of oocysts. There was a significant increase in the percentage of oocysts that took up propidium iodide and in the number of oocysts that excysted spontaneously. There was also a significant decrease in the intensity of infection elicited in suckling mice at the end of all exposure times. The results of the study demonstrate the importance of temperature in the inactivation of C. parvum oocysts during application of SODIS under natural conditions.

Authors+Show Affiliations

Laboratorio de Parasitología, Departamento de Microbiología y Parasitología, Facultad de Farmacia, Universidad de Santiago de Compostela, Santiago de Compostela, A Coruña, Spain.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20064992

Citation

Gómez-Couso, Hipólito, et al. "Thermal Contribution to the Inactivation of Cryptosporidium in Plastic Bottles During Solar Water Disinfection Procedures." The American Journal of Tropical Medicine and Hygiene, vol. 82, no. 1, 2010, pp. 35-9.
Gómez-Couso H, Fontán-Sainz M, Ares-Mazás E. Thermal contribution to the inactivation of Cryptosporidium in plastic bottles during solar water disinfection procedures. Am J Trop Med Hyg. 2010;82(1):35-9.
Gómez-Couso, H., Fontán-Sainz, M., & Ares-Mazás, E. (2010). Thermal contribution to the inactivation of Cryptosporidium in plastic bottles during solar water disinfection procedures. The American Journal of Tropical Medicine and Hygiene, 82(1), 35-9. https://doi.org/10.4269/ajtmh.2010.09-0284
Gómez-Couso H, Fontán-Sainz M, Ares-Mazás E. Thermal Contribution to the Inactivation of Cryptosporidium in Plastic Bottles During Solar Water Disinfection Procedures. Am J Trop Med Hyg. 2010;82(1):35-9. PubMed PMID: 20064992.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Thermal contribution to the inactivation of Cryptosporidium in plastic bottles during solar water disinfection procedures. AU - Gómez-Couso,Hipólito, AU - Fontán-Sainz,María, AU - Ares-Mazás,Elvira, PY - 2010/1/13/entrez PY - 2010/1/13/pubmed PY - 2010/1/26/medline SP - 35 EP - 9 JF - The American journal of tropical medicine and hygiene JO - Am J Trop Med Hyg VL - 82 IS - 1 N2 - To determine the thermal contribution, independent of ultraviolet radiation, on the inactivation of Cryptosporidium parvum during solar water disinfection procedures (SODIS), oocysts were exposed for 4, 8, and 12 hours to temperatures recorded in polyethylene terephthalate bottles in previous SODIS studies carried out under field conditions. Inclusion/exclusion of the fluorogenic vital dye propidium iodide, spontaneous excystation, and infectivity studies were used to determine the inactivation of oocysts. There was a significant increase in the percentage of oocysts that took up propidium iodide and in the number of oocysts that excysted spontaneously. There was also a significant decrease in the intensity of infection elicited in suckling mice at the end of all exposure times. The results of the study demonstrate the importance of temperature in the inactivation of C. parvum oocysts during application of SODIS under natural conditions. SN - 1476-1645 UR - https://www.unboundmedicine.com/medline/citation/20064992/Thermal_contribution_to_the_inactivation_of_Cryptosporidium_in_plastic_bottles_during_solar_water_disinfection_procedures_ L2 - https://ajtmh.org/doi/10.4269/ajtmh.2010.09-0284 DB - PRIME DP - Unbound Medicine ER -