Abstract
OBJECTIVE
To establish the analytical method of the HPLC fingerprint of Cortex Moutan Carbonisatum and offer the evidence for quality control and quality evaluation of carbonized Cortex Moutan accordingly.
METHOD
Ten batches of Cortex Moutan Carbonisatum were measured by HPLC with Paeonol as a reference substance and the HPLC analysis was performed on a Hedera ODS-3 Chromatographic Column (4.6 mm x 150 mm, 5 microm), with methanol to Acetonitrile (1:1) and 0.5% trifluoroacetic acid solution as mobile phase in gradient mode and the detection wavelength was at 258 nm.
RESULT
The common mode of the HPLC fingerprints were set up. There were 11 common peaks in the fingerprint of ten samples, and the similar degrees to the ten batches were between 0.830-0.991.
CONCLUSION
The method is simple, accurate and have a good reaptability. And the quality of Cortex Moutan Carbonisatum can be controlled effectively by the HPLC fingerprint.
TY - JOUR
T1 - [HPLC fingerprints of Cortex Moutan Carbonisatum].
AU - Li,Peng,
AU - Zhao,Xuelong,
AU - Zhang,Li,
AU - Ding,Anwei,
PY - 2010/1/14/entrez
PY - 2010/1/14/pubmed
PY - 2010/1/29/medline
SP - 2463
EP - 5
JF - Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica
JO - Zhongguo Zhong Yao Za Zhi
VL - 34
IS - 19
N2 - OBJECTIVE: To establish the analytical method of the HPLC fingerprint of Cortex Moutan Carbonisatum and offer the evidence for quality control and quality evaluation of carbonized Cortex Moutan accordingly. METHOD: Ten batches of Cortex Moutan Carbonisatum were measured by HPLC with Paeonol as a reference substance and the HPLC analysis was performed on a Hedera ODS-3 Chromatographic Column (4.6 mm x 150 mm, 5 microm), with methanol to Acetonitrile (1:1) and 0.5% trifluoroacetic acid solution as mobile phase in gradient mode and the detection wavelength was at 258 nm. RESULT: The common mode of the HPLC fingerprints were set up. There were 11 common peaks in the fingerprint of ten samples, and the similar degrees to the ten batches were between 0.830-0.991. CONCLUSION: The method is simple, accurate and have a good reaptability. And the quality of Cortex Moutan Carbonisatum can be controlled effectively by the HPLC fingerprint.
SN - 1001-5302
UR - https://www.unboundmedicine.com/medline/citation/20067013/[HPLC_fingerprints_of_Cortex_Moutan_Carbonisatum]_
DB - PRIME
DP - Unbound Medicine
ER -
