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[Acanthopanax senticosus saponins ameliorates oxidative damage induced by hydrogen peroxide in neonatal rat cardiomyocytes].
Zhongguo Zhong Yao Za Zhi. 2009 Oct; 34(19):2489-93.ZZ

Abstract

OBJECTIVE

To investigate the effect of Acanthopanax senticosus saponins (ASS) on oxidative damage induced by hydrogen peroxide (H2O2) in cardiomyocytes.

METHOD

The cardiomyocytes were induced to oxidative damaged by exposed to H2O2. We evaluated the level of oxidative injury through morphology change, the survival rate, the activity of lactate dehydrogenase (LDH) and the content of cellular malondialdehyde (MDA). The activities of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), catalase (CAT) and the content of reductive glutathione (GSH) of cardiomyocytes were also determined.

RESULT

When the cardiomyocytes were exposed to H2O, (50, 100, 200 micromol x L(-1)) for deigned time, the percentage of survival cells was down significantly (P < 0.01 or P < 0.001), and the activity of LDH and the content of MDA were increased markedly (P < 0.05, P < 0.01 or P < 0.001). These results show that the cells were subjected to oxidative damage. Treatment with ASS (600 mg x L(-1)) prior to H2O2 exposure could increase cell viability significantly, lessen cardiomyocyte morphological damaged change, and decrease LDH activity (1687.40 +/- 97.51) U x mL(-1) in media and cellular MDA content (16.50 +/- 2.66) nmol x mg(-1) markedly (P < 0.01 and P < 0.05). Furthermore, the activities of SOD (89.55 +/- 6.93) U x mg(-1), GSH-Px (845.87 +/- 63.76) mU x mg(-1), CAT (93.07 +/- 10.40) U x mg(-1) and the content of GSH (8.91 +/- 1.06) micromol x mg(-1) of cardiomyocytes were also raised by 600 mg x L(-1) l ASS (P < 0.05).

CONCLUSION

Taken together, the study implicate that ASS protects cardiomyocytes against oxidative-stress injury induced by H2O2 through reduction of lipid peroxidation and enhancement of the activity of antioxidant defense.

Authors+Show Affiliations

Department of Pharmacology, School of Pharmaceutical Sciences, Jilin University, Changchun 130021, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

English Abstract
Journal Article
Research Support, Non-U.S. Gov't

Language

chi

PubMed ID

20067020

Citation

Liang, Qiming, et al. "[Acanthopanax Senticosus Saponins Ameliorates Oxidative Damage Induced By Hydrogen Peroxide in Neonatal Rat Cardiomyocytes]." Zhongguo Zhong Yao Za Zhi = Zhongguo Zhongyao Zazhi = China Journal of Chinese Materia Medica, vol. 34, no. 19, 2009, pp. 2489-93.
Liang Q, Qu S, Yu X, et al. [Acanthopanax senticosus saponins ameliorates oxidative damage induced by hydrogen peroxide in neonatal rat cardiomyocytes]. Zhongguo Zhong Yao Za Zhi. 2009;34(19):2489-93.
Liang, Q., Qu, S., Yu, X., Xu, H., & Sui, D. (2009). [Acanthopanax senticosus saponins ameliorates oxidative damage induced by hydrogen peroxide in neonatal rat cardiomyocytes]. Zhongguo Zhong Yao Za Zhi = Zhongguo Zhongyao Zazhi = China Journal of Chinese Materia Medica, 34(19), 2489-93.
Liang Q, et al. [Acanthopanax Senticosus Saponins Ameliorates Oxidative Damage Induced By Hydrogen Peroxide in Neonatal Rat Cardiomyocytes]. Zhongguo Zhong Yao Za Zhi. 2009;34(19):2489-93. PubMed PMID: 20067020.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Acanthopanax senticosus saponins ameliorates oxidative damage induced by hydrogen peroxide in neonatal rat cardiomyocytes]. AU - Liang,Qiming, AU - Qu,Shaochun, AU - Yu,Xiaofeng, AU - Xu,Huali, AU - Sui,Dayun, PY - 2010/1/14/entrez PY - 2010/1/14/pubmed PY - 2010/1/29/medline SP - 2489 EP - 93 JF - Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica JO - Zhongguo Zhong Yao Za Zhi VL - 34 IS - 19 N2 - OBJECTIVE: To investigate the effect of Acanthopanax senticosus saponins (ASS) on oxidative damage induced by hydrogen peroxide (H2O2) in cardiomyocytes. METHOD: The cardiomyocytes were induced to oxidative damaged by exposed to H2O2. We evaluated the level of oxidative injury through morphology change, the survival rate, the activity of lactate dehydrogenase (LDH) and the content of cellular malondialdehyde (MDA). The activities of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), catalase (CAT) and the content of reductive glutathione (GSH) of cardiomyocytes were also determined. RESULT: When the cardiomyocytes were exposed to H2O, (50, 100, 200 micromol x L(-1)) for deigned time, the percentage of survival cells was down significantly (P < 0.01 or P < 0.001), and the activity of LDH and the content of MDA were increased markedly (P < 0.05, P < 0.01 or P < 0.001). These results show that the cells were subjected to oxidative damage. Treatment with ASS (600 mg x L(-1)) prior to H2O2 exposure could increase cell viability significantly, lessen cardiomyocyte morphological damaged change, and decrease LDH activity (1687.40 +/- 97.51) U x mL(-1) in media and cellular MDA content (16.50 +/- 2.66) nmol x mg(-1) markedly (P < 0.01 and P < 0.05). Furthermore, the activities of SOD (89.55 +/- 6.93) U x mg(-1), GSH-Px (845.87 +/- 63.76) mU x mg(-1), CAT (93.07 +/- 10.40) U x mg(-1) and the content of GSH (8.91 +/- 1.06) micromol x mg(-1) of cardiomyocytes were also raised by 600 mg x L(-1) l ASS (P < 0.05). CONCLUSION: Taken together, the study implicate that ASS protects cardiomyocytes against oxidative-stress injury induced by H2O2 through reduction of lipid peroxidation and enhancement of the activity of antioxidant defense. SN - 1001-5302 UR - https://www.unboundmedicine.com/medline/citation/20067020/[Acanthopanax_senticosus_saponins_ameliorates_oxidative_damage_induced_by_hydrogen_peroxide_in_neonatal_rat_cardiomyocytes]_ L2 - https://antibodies.cancer.gov/detail/CPTC-GSTMu1-6 DB - PRIME DP - Unbound Medicine ER -