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[Research on phytoestrogenic effects and their mechanisms of Jiaoai tang and Shenqi Jiaoai tang].
Zhongguo Zhong Yao Za Zhi. 2009 Oct; 34(19):2503-7.ZZ

Abstract

OBJECTIVE

To study the phytoestrogenic effects and their possible mechanisms of Jiaoai tang and Shenqi Jiaoai tang through the tests in mice and ER (+) MCF7 cells.

METHOD

Sixty kunming mice weighing 9-12 g were randomly divided into 6 groups: solvent control group (administrated equal dose of diswater), diethylstilbestrol control group (administrated diethylstilbestrol at a dose of 0.35 mg x kg(-1) x d(-1) and 4 Chinese medicine treated groups (administrated low or high doses of Jiaoai tang and Shenqi Jiaoai tang at 2.5 g x kg(-1) x d(-1) or 5.0 g x kg(-1) x d(-1) respectively). After administration for 4 days, the mice were sacrificed; uterus was removed and weighed, uterus rate was calculated. The blood serum was also separated. The proliferation rate of MCF7 cells influenced by Jiaoai tang and Shenqi Jiaoai tang was determined by MIT assay. PS2, ERalpha and ERbeta mRNA expression was quantified by Real-time PCR assay. Estrogen receptor antagonist ICI182, 780 was employed as a tool.

RESULT

Administration of Jiaoai tang and Shenqi Jiaoai tang at high dose significantly increased uterus rate in mice (P < 0. 05). The pharmacological serum from two high-dosage groups of Chinese herbal medicine decoction significantly enhanced proliferation rate of MCF7 cells (P < 0.05 or 0.01), while their effects were blocked by ICI182, 780 (P < 0.05 or 0.01). The pharmacological serum could cause elevation of pS2 level (P < 0.01) which would be obviously inhibited by ICI182, 780 (P < 0.01). ERalpha and ERbeta mRNA levels were also elevated significantly (P < 0.05 and P < 0.01 respectively).

CONCLUSION

Jiaoai tang and Shenqi Jiaoai tang have phytoestrogenic effects, which were attained via ER pathway. They can also increase the mRNA levels of estrogen receptor subtypes, especially ERbeta.

Authors+Show Affiliations

Beijing University of Chinese Medicine, Beijing 100029, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

English Abstract
Journal Article
Research Support, Non-U.S. Gov't

Language

chi

PubMed ID

20067023

Citation

Zhao, Piwen, et al. "[Research On Phytoestrogenic Effects and Their Mechanisms of Jiaoai Tang and Shenqi Jiaoai Tang]." Zhongguo Zhong Yao Za Zhi = Zhongguo Zhongyao Zazhi = China Journal of Chinese Materia Medica, vol. 34, no. 19, 2009, pp. 2503-7.
Zhao P, Niu J, Wang J, et al. [Research on phytoestrogenic effects and their mechanisms of Jiaoai tang and Shenqi Jiaoai tang]. Zhongguo Zhong Yao Za Zhi. 2009;34(19):2503-7.
Zhao, P., Niu, J., Wang, J., Yu, J., Hao, Q., & Li, Y. (2009). [Research on phytoestrogenic effects and their mechanisms of Jiaoai tang and Shenqi Jiaoai tang]. Zhongguo Zhong Yao Za Zhi = Zhongguo Zhongyao Zazhi = China Journal of Chinese Materia Medica, 34(19), 2503-7.
Zhao P, et al. [Research On Phytoestrogenic Effects and Their Mechanisms of Jiaoai Tang and Shenqi Jiaoai Tang]. Zhongguo Zhong Yao Za Zhi. 2009;34(19):2503-7. PubMed PMID: 20067023.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Research on phytoestrogenic effects and their mechanisms of Jiaoai tang and Shenqi Jiaoai tang]. AU - Zhao,Piwen, AU - Niu,Jianzhao, AU - Wang,Jifeng, AU - Yu,Jie, AU - Hao,Qingxiu, AU - Li,Yadong, PY - 2010/1/14/entrez PY - 2010/1/14/pubmed PY - 2010/1/29/medline SP - 2503 EP - 7 JF - Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica JO - Zhongguo Zhong Yao Za Zhi VL - 34 IS - 19 N2 - OBJECTIVE: To study the phytoestrogenic effects and their possible mechanisms of Jiaoai tang and Shenqi Jiaoai tang through the tests in mice and ER (+) MCF7 cells. METHOD: Sixty kunming mice weighing 9-12 g were randomly divided into 6 groups: solvent control group (administrated equal dose of diswater), diethylstilbestrol control group (administrated diethylstilbestrol at a dose of 0.35 mg x kg(-1) x d(-1) and 4 Chinese medicine treated groups (administrated low or high doses of Jiaoai tang and Shenqi Jiaoai tang at 2.5 g x kg(-1) x d(-1) or 5.0 g x kg(-1) x d(-1) respectively). After administration for 4 days, the mice were sacrificed; uterus was removed and weighed, uterus rate was calculated. The blood serum was also separated. The proliferation rate of MCF7 cells influenced by Jiaoai tang and Shenqi Jiaoai tang was determined by MIT assay. PS2, ERalpha and ERbeta mRNA expression was quantified by Real-time PCR assay. Estrogen receptor antagonist ICI182, 780 was employed as a tool. RESULT: Administration of Jiaoai tang and Shenqi Jiaoai tang at high dose significantly increased uterus rate in mice (P < 0. 05). The pharmacological serum from two high-dosage groups of Chinese herbal medicine decoction significantly enhanced proliferation rate of MCF7 cells (P < 0.05 or 0.01), while their effects were blocked by ICI182, 780 (P < 0.05 or 0.01). The pharmacological serum could cause elevation of pS2 level (P < 0.01) which would be obviously inhibited by ICI182, 780 (P < 0.01). ERalpha and ERbeta mRNA levels were also elevated significantly (P < 0.05 and P < 0.01 respectively). CONCLUSION: Jiaoai tang and Shenqi Jiaoai tang have phytoestrogenic effects, which were attained via ER pathway. They can also increase the mRNA levels of estrogen receptor subtypes, especially ERbeta. SN - 1001-5302 UR - https://www.unboundmedicine.com/medline/citation/20067023/[Research_on_phytoestrogenic_effects_and_their_mechanisms_of_Jiaoai_tang_and_Shenqi_Jiaoai_tang]_ DB - PRIME DP - Unbound Medicine ER -