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Expression analysis of angiogenic growth factors and biological axis CXCL12/CXCR4 axis in idiopathic pulmonary fibrosis.
Connect Tissue Res. 2010; 51(1):71-80.CT

Abstract

Idiopathic pulmonary fibrosis (IPF) is associated with aberrant repair, persistence of collagen deposition, and the development of vascular remodeling. However, the role of angiogenesis in the pathogenesis of IPF is still undetermined. The aim of this study was to evaluate the combined mRNA expression of vascular endothelial growth factor A (VEGFA), fibroblast growth factor 2 (FGF2), insulin-like growth factor 1 (IGF1) epidermal growth factor (EGF), and its receptor (EGFR) in lung tissue obtained from IPF patients. We have also investigated the expression of chemokine CXCL12/stromal cell-derived factor-1 (SDF-1) and its receptor, CXCR4, to identify alterations that maybe implicated in the pathogenesis of IPF. The subjects studied consisted of two distinct groups: patients with IPF (n = 25) and subjects (control) undergoing thoracic surgery for reasons other than interstitial lung disease (n = 10). Expression analysis of the aforementioned growth factors and biological axis CXCL12/CXR4 analysis were performed using real-time RT-PCR. IGF-1, EGF, and FGF2 mRNA levels are significantly decreased in the patients compared to the controls (p = 0.028, p = 0.023 and p = 0.009, respectively). SDF1-TR1 and SDF1-TR2 transcript levels were significantly lower in patients compared to controls (p = 0.017 and p = 0.001). Significant coexpression of VEGF mRNA with IGF mRNA was observed in the group of the patients (p = 0.017). An additional coexpression of VEGF mRNA with SDF1-TR1 mRNA was demonstrated(p = 0.030). Our results show a downregulation in angiogenetic mechanisms in IPF. However, our results should be further verified by measuring other angiogenetic pathways in more samples.

Authors+Show Affiliations

Department of Thoracic Medicine, Interstitial Lung Disease Unit, Medical School, University of Crete, Heraklion, Crete, Greece.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

20067420

Citation

Antoniou, Katerina M., et al. "Expression Analysis of Angiogenic Growth Factors and Biological Axis CXCL12/CXCR4 Axis in Idiopathic Pulmonary Fibrosis." Connective Tissue Research, vol. 51, no. 1, 2010, pp. 71-80.
Antoniou KM, Soufla G, Lymbouridou R, et al. Expression analysis of angiogenic growth factors and biological axis CXCL12/CXCR4 axis in idiopathic pulmonary fibrosis. Connect Tissue Res. 2010;51(1):71-80.
Antoniou, K. M., Soufla, G., Lymbouridou, R., Economidou, F., Lasithiotaki, I., Manousakis, M., Drositis, I., Spandidos, D. A., & Siafakas, N. M. (2010). Expression analysis of angiogenic growth factors and biological axis CXCL12/CXCR4 axis in idiopathic pulmonary fibrosis. Connective Tissue Research, 51(1), 71-80. https://doi.org/10.3109/03008200903056150
Antoniou KM, et al. Expression Analysis of Angiogenic Growth Factors and Biological Axis CXCL12/CXCR4 Axis in Idiopathic Pulmonary Fibrosis. Connect Tissue Res. 2010;51(1):71-80. PubMed PMID: 20067420.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Expression analysis of angiogenic growth factors and biological axis CXCL12/CXCR4 axis in idiopathic pulmonary fibrosis. AU - Antoniou,Katerina M, AU - Soufla,Giannoula, AU - Lymbouridou,Rena, AU - Economidou,Foteini, AU - Lasithiotaki,Ismini, AU - Manousakis,Manolis, AU - Drositis,Ioannis, AU - Spandidos,Demetrios A, AU - Siafakas,Nikolaos M, PY - 2010/1/14/entrez PY - 2010/1/14/pubmed PY - 2010/4/7/medline SP - 71 EP - 80 JF - Connective tissue research JO - Connect Tissue Res VL - 51 IS - 1 N2 - Idiopathic pulmonary fibrosis (IPF) is associated with aberrant repair, persistence of collagen deposition, and the development of vascular remodeling. However, the role of angiogenesis in the pathogenesis of IPF is still undetermined. The aim of this study was to evaluate the combined mRNA expression of vascular endothelial growth factor A (VEGFA), fibroblast growth factor 2 (FGF2), insulin-like growth factor 1 (IGF1) epidermal growth factor (EGF), and its receptor (EGFR) in lung tissue obtained from IPF patients. We have also investigated the expression of chemokine CXCL12/stromal cell-derived factor-1 (SDF-1) and its receptor, CXCR4, to identify alterations that maybe implicated in the pathogenesis of IPF. The subjects studied consisted of two distinct groups: patients with IPF (n = 25) and subjects (control) undergoing thoracic surgery for reasons other than interstitial lung disease (n = 10). Expression analysis of the aforementioned growth factors and biological axis CXCL12/CXR4 analysis were performed using real-time RT-PCR. IGF-1, EGF, and FGF2 mRNA levels are significantly decreased in the patients compared to the controls (p = 0.028, p = 0.023 and p = 0.009, respectively). SDF1-TR1 and SDF1-TR2 transcript levels were significantly lower in patients compared to controls (p = 0.017 and p = 0.001). Significant coexpression of VEGF mRNA with IGF mRNA was observed in the group of the patients (p = 0.017). An additional coexpression of VEGF mRNA with SDF1-TR1 mRNA was demonstrated(p = 0.030). Our results show a downregulation in angiogenetic mechanisms in IPF. However, our results should be further verified by measuring other angiogenetic pathways in more samples. SN - 1607-8438 UR - https://www.unboundmedicine.com/medline/citation/20067420/Expression_analysis_of_angiogenic_growth_factors_and_biological_axis_CXCL12/CXCR4_axis_in_idiopathic_pulmonary_fibrosis_ L2 - https://www.tandfonline.com/doi/full/10.3109/03008200903056150 DB - PRIME DP - Unbound Medicine ER -