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Activation and induction of cytosolic phospholipase A2 by IL-1beta in human tracheal smooth muscle cells: role of MAPKs/p300 and NF-kappaB.
J Cell Biochem. 2010 Apr 01; 109(5):1045-56.JC

Abstract

Cytosolic phospholipase A(2) (cPLA(2)) plays a pivotal role in mediating agonist-induced arachidonic acid (AA) release for prostaglandin (PG) synthesis during inflammation triggered by IL-1beta. However, the mechanisms underlying IL-1beta-induced cPLA(2) expression and PGE(2) synthesis in human tracheal smooth muscle cells (HTSMCs) remain unknown. IL-1beta-induced cPLA(2) protein and mRNA expression, PGE(2) production, or phosphorylation of p42/p44 MAPK, p38 MAPK, and JNK1/2, which was attenuated by pretreatment with the inhibitors of MEK1/2 (U0126), p38 MAPK (SB202190), and JNK1/2 (SP600125) or transfection with siRNAs of MEK1, p42, p38, and JNK2. IL-1beta-induced cPLA(2) expression was also inhibited by pretreatment with a NF-kappaB inhibitor, helenalin or transfection with siRNA of NIK, IKKalpha, or IKKbeta. IL-beta-induced NF-kappaB translocation was blocked by pretreatment with helenalin, but not U0126, SB202190, and SP600125. In addition, transfection with p300 siRNA blocked cPLA(2) expression induced by IL-1beta. Moreover, p300 was associated with the cPLA(2) promoter, which was dynamically linked to histone H4 acetylation stimulated by IL-1beta. These results suggest that in HTSMCs, activation of MAPKs, NF-kappaB, and p300 are essential for IL-1beta-induced cPLA(2) expression and PGE(2) secretion.

Authors+Show Affiliations

Division of Basic Medical Sciences, Department of Nursing, Chang Gung Institute of Technology, Chia-Yi, Taiwan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20069553

Citation

Lee, Chiang-Wen, et al. "Activation and Induction of Cytosolic Phospholipase A2 By IL-1beta in Human Tracheal Smooth Muscle Cells: Role of MAPKs/p300 and NF-kappaB." Journal of Cellular Biochemistry, vol. 109, no. 5, 2010, pp. 1045-56.
Lee CW, Lee IT, Lin CC, et al. Activation and induction of cytosolic phospholipase A2 by IL-1beta in human tracheal smooth muscle cells: role of MAPKs/p300 and NF-kappaB. J Cell Biochem. 2010;109(5):1045-56.
Lee, C. W., Lee, I. T., Lin, C. C., Lee, H. C., Lin, W. N., & Yang, C. M. (2010). Activation and induction of cytosolic phospholipase A2 by IL-1beta in human tracheal smooth muscle cells: role of MAPKs/p300 and NF-kappaB. Journal of Cellular Biochemistry, 109(5), 1045-56. https://doi.org/10.1002/jcb.22488
Lee CW, et al. Activation and Induction of Cytosolic Phospholipase A2 By IL-1beta in Human Tracheal Smooth Muscle Cells: Role of MAPKs/p300 and NF-kappaB. J Cell Biochem. 2010 Apr 1;109(5):1045-56. PubMed PMID: 20069553.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Activation and induction of cytosolic phospholipase A2 by IL-1beta in human tracheal smooth muscle cells: role of MAPKs/p300 and NF-kappaB. AU - Lee,Chiang-Wen, AU - Lee,I-Ta, AU - Lin,Chih-Chung, AU - Lee,Hui-Chun, AU - Lin,Wei-Ning, AU - Yang,Chuen-Mao, PY - 2010/1/14/entrez PY - 2010/1/14/pubmed PY - 2010/6/26/medline SP - 1045 EP - 56 JF - Journal of cellular biochemistry JO - J. Cell. Biochem. VL - 109 IS - 5 N2 - Cytosolic phospholipase A(2) (cPLA(2)) plays a pivotal role in mediating agonist-induced arachidonic acid (AA) release for prostaglandin (PG) synthesis during inflammation triggered by IL-1beta. However, the mechanisms underlying IL-1beta-induced cPLA(2) expression and PGE(2) synthesis in human tracheal smooth muscle cells (HTSMCs) remain unknown. IL-1beta-induced cPLA(2) protein and mRNA expression, PGE(2) production, or phosphorylation of p42/p44 MAPK, p38 MAPK, and JNK1/2, which was attenuated by pretreatment with the inhibitors of MEK1/2 (U0126), p38 MAPK (SB202190), and JNK1/2 (SP600125) or transfection with siRNAs of MEK1, p42, p38, and JNK2. IL-1beta-induced cPLA(2) expression was also inhibited by pretreatment with a NF-kappaB inhibitor, helenalin or transfection with siRNA of NIK, IKKalpha, or IKKbeta. IL-beta-induced NF-kappaB translocation was blocked by pretreatment with helenalin, but not U0126, SB202190, and SP600125. In addition, transfection with p300 siRNA blocked cPLA(2) expression induced by IL-1beta. Moreover, p300 was associated with the cPLA(2) promoter, which was dynamically linked to histone H4 acetylation stimulated by IL-1beta. These results suggest that in HTSMCs, activation of MAPKs, NF-kappaB, and p300 are essential for IL-1beta-induced cPLA(2) expression and PGE(2) secretion. SN - 1097-4644 UR - https://www.unboundmedicine.com/medline/citation/20069553/Activation_and_induction_of_cytosolic_phospholipase_A2_by_IL_1beta_in_human_tracheal_smooth_muscle_cells:_role_of_MAPKs/p300_and_NF_kappaB_ L2 - https://doi.org/10.1002/jcb.22488 DB - PRIME DP - Unbound Medicine ER -