Tags

Type your tag names separated by a space and hit enter

The Skp1-like protein SSK1 is required for cross-pollen compatibility in S-RNase-based self-incompatibility.
Plant J. 2010 Apr 01; 62(1):52-63.PJ

Abstract

The self-incompatibility (SI) response occurs widely in flowering plants as a means of preventing self-fertilization. In these self/non-self discrimination systems, plant pistils reject self or genetically related pollen. In the Solanaceae, Plantaginaceae and Rosaceae, pistil-secreted S-RNases enter the pollen tube and function as cytotoxins to specifically arrest self-pollen tube growth. Recent studies have revealed that the S-locus F-box (SLF) protein controls the pollen expression of SI in these families. However, the precise role of SLF remains largely unknown. Here we report that PhSSK1 (Petunia hybrida SLF-interacting Skp1-like1), an equivalent of AhSSK1 of Antirrhinum hispanicum, is expressed specifically in pollen and acts as an adaptor in an SCF(Skp1-Cullin1-F-box)(SLF) complex, indicating that this pollen-specific SSK1-SLF interaction occurs in both Petunia and Antirrhinum, two species from the Solanaceae and Plantaginaceae, respectively. Substantial reduction of PhSSK1 in pollen reduced cross-pollen compatibility (CPC) in the S-RNase-based SI response, suggesting that the pollen S determinant contributes to inhibiting rather than protecting the S-RNase activity, at least in solanaceous plants. Furthermore, our results provide an example that a specific Skp1-like protein other than the known conserved ones can be recruited into a canonical SCF complex as an adaptor.

Authors+Show Affiliations

Laboratory of Molecular and Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences and National Center for Plant Gene Research, Beijing 100190, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20070569

Citation

Zhao, Lan, et al. "The Skp1-like Protein SSK1 Is Required for Cross-pollen Compatibility in S-RNase-based Self-incompatibility." The Plant Journal : for Cell and Molecular Biology, vol. 62, no. 1, 2010, pp. 52-63.
Zhao L, Huang J, Zhao Z, et al. The Skp1-like protein SSK1 is required for cross-pollen compatibility in S-RNase-based self-incompatibility. Plant J. 2010;62(1):52-63.
Zhao, L., Huang, J., Zhao, Z., Li, Q., Sims, T. L., & Xue, Y. (2010). The Skp1-like protein SSK1 is required for cross-pollen compatibility in S-RNase-based self-incompatibility. The Plant Journal : for Cell and Molecular Biology, 62(1), 52-63. https://doi.org/10.1111/j.1365-313X.2010.04123.x
Zhao L, et al. The Skp1-like Protein SSK1 Is Required for Cross-pollen Compatibility in S-RNase-based Self-incompatibility. Plant J. 2010 Apr 1;62(1):52-63. PubMed PMID: 20070569.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The Skp1-like protein SSK1 is required for cross-pollen compatibility in S-RNase-based self-incompatibility. AU - Zhao,Lan, AU - Huang,Jian, AU - Zhao,Zhonghua, AU - Li,Qun, AU - Sims,Thomas L, AU - Xue,Yongbiao, Y1 - 2010/01/07/ PY - 2010/1/15/entrez PY - 2010/1/15/pubmed PY - 2010/7/17/medline SP - 52 EP - 63 JF - The Plant journal : for cell and molecular biology JO - Plant J VL - 62 IS - 1 N2 - The self-incompatibility (SI) response occurs widely in flowering plants as a means of preventing self-fertilization. In these self/non-self discrimination systems, plant pistils reject self or genetically related pollen. In the Solanaceae, Plantaginaceae and Rosaceae, pistil-secreted S-RNases enter the pollen tube and function as cytotoxins to specifically arrest self-pollen tube growth. Recent studies have revealed that the S-locus F-box (SLF) protein controls the pollen expression of SI in these families. However, the precise role of SLF remains largely unknown. Here we report that PhSSK1 (Petunia hybrida SLF-interacting Skp1-like1), an equivalent of AhSSK1 of Antirrhinum hispanicum, is expressed specifically in pollen and acts as an adaptor in an SCF(Skp1-Cullin1-F-box)(SLF) complex, indicating that this pollen-specific SSK1-SLF interaction occurs in both Petunia and Antirrhinum, two species from the Solanaceae and Plantaginaceae, respectively. Substantial reduction of PhSSK1 in pollen reduced cross-pollen compatibility (CPC) in the S-RNase-based SI response, suggesting that the pollen S determinant contributes to inhibiting rather than protecting the S-RNase activity, at least in solanaceous plants. Furthermore, our results provide an example that a specific Skp1-like protein other than the known conserved ones can be recruited into a canonical SCF complex as an adaptor. SN - 1365-313X UR - https://www.unboundmedicine.com/medline/citation/20070569/The_Skp1_like_protein_SSK1_is_required_for_cross_pollen_compatibility_in_S_RNase_based_self_incompatibility_ DB - PRIME DP - Unbound Medicine ER -