Tags

Type your tag names separated by a space and hit enter

ANG II receptor blockade enhances anti-inflammatory macrophages in anti-glomerular basement membrane glomerulonephritis.
Am J Physiol Renal Physiol. 2010 Apr; 298(4):F870-82.AJ

Abstract

Macrophages are heterogeneous immune cell populations that include classically activated and alternatively activated (M2) macrophages. We examined the anti-inflammatory effect of ANG II type 1 receptor (AT(1)R) blocker (ARB) on glomerular inflammation in a rat model of anti-glomerular basement membrane (GBM) glomerulonephritis (GN). The study focused on infiltrating CD8(+) and CD4(+) cells and macrophages, as well as the heterogeneity of intraglomerular macrophages. Wistar-Kyoto rats were treated with high-dose olmesartan (3 mg.kg(-1).day(-1)), low-dose olmesartan (0.3 mg.kg(-1).day(-1)), or vehicle (control) 7 days before induction of anti-GBM GN. Control rats showed mainly CD8(+) cells and ED1(+) macrophages, with a few CD4(+) cells infiltrating the glomeruli. Necrotizing and crescentic glomerular lesions developed by day 7 with the increase of proteinuria. AT(1)R was expressed on CD8(+) and CD4(+) cells and on ED1(+) macrophages. Low-dose ARB had no anti-inflammatory effects in anti-GBM GN. However, high-dose ARB reduced glomerular infiltration of CD8(+) cells and ED1(+) macrophages and suppressed necrotizing and crescentic lesions by days 5 to 7 (P < 0.05). In addition, high-dose ARB reduced the numbers of ED3(+)-activated macrophages, suppressed glomerular TNF-alpha and IFN-gamma production, and downregulated M1-related chemokine and cytokines (monocyte chemoattractant protein type 1, IL-6, and IL-12). High-dose ARB also enhanced ED2(+) M2 macrophages by day 7 with upregulation of glomerular IL-4 and IL-13 and augmented CCL17, IL-1 receptor antagonist, and IL-10. We concluded that high-dose ARB inhibits glomerular inflammation by increasing the numbers of M2 macrophages and upregulation of anti-inflammatory cytokines and by suppressing M1 macrophage development with downregulation of M1-related proinflammatory cytokines.

Authors+Show Affiliations

Departmentsof Analytic Human Pathology, Nippon Medical School, 1-1-5 Sendagi, Bunkyo-ku, Tokyo, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

20071465

Citation

Aki, Kaoru, et al. "ANG II Receptor Blockade Enhances Anti-inflammatory Macrophages in Anti-glomerular Basement Membrane Glomerulonephritis." American Journal of Physiology. Renal Physiology, vol. 298, no. 4, 2010, pp. F870-82.
Aki K, Shimizu A, Masuda Y, et al. ANG II receptor blockade enhances anti-inflammatory macrophages in anti-glomerular basement membrane glomerulonephritis. Am J Physiol Renal Physiol. 2010;298(4):F870-82.
Aki, K., Shimizu, A., Masuda, Y., Kuwahara, N., Arai, T., Ishikawa, A., Fujita, E., Mii, A., Natori, Y., Fukunaga, Y., & Fukuda, Y. (2010). ANG II receptor blockade enhances anti-inflammatory macrophages in anti-glomerular basement membrane glomerulonephritis. American Journal of Physiology. Renal Physiology, 298(4), F870-82. https://doi.org/10.1152/ajprenal.00374.2009
Aki K, et al. ANG II Receptor Blockade Enhances Anti-inflammatory Macrophages in Anti-glomerular Basement Membrane Glomerulonephritis. Am J Physiol Renal Physiol. 2010;298(4):F870-82. PubMed PMID: 20071465.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - ANG II receptor blockade enhances anti-inflammatory macrophages in anti-glomerular basement membrane glomerulonephritis. AU - Aki,Kaoru, AU - Shimizu,Akira, AU - Masuda,Yukinari, AU - Kuwahara,Naomi, AU - Arai,Takashi, AU - Ishikawa,Arimi, AU - Fujita,Emiko, AU - Mii,Akiko, AU - Natori,Yasuhiro, AU - Fukunaga,Yoshitaka, AU - Fukuda,Yuh, Y1 - 2010/01/13/ PY - 2010/1/15/entrez PY - 2010/1/15/pubmed PY - 2010/4/23/medline SP - F870 EP - 82 JF - American journal of physiology. Renal physiology JO - Am. J. Physiol. Renal Physiol. VL - 298 IS - 4 N2 - Macrophages are heterogeneous immune cell populations that include classically activated and alternatively activated (M2) macrophages. We examined the anti-inflammatory effect of ANG II type 1 receptor (AT(1)R) blocker (ARB) on glomerular inflammation in a rat model of anti-glomerular basement membrane (GBM) glomerulonephritis (GN). The study focused on infiltrating CD8(+) and CD4(+) cells and macrophages, as well as the heterogeneity of intraglomerular macrophages. Wistar-Kyoto rats were treated with high-dose olmesartan (3 mg.kg(-1).day(-1)), low-dose olmesartan (0.3 mg.kg(-1).day(-1)), or vehicle (control) 7 days before induction of anti-GBM GN. Control rats showed mainly CD8(+) cells and ED1(+) macrophages, with a few CD4(+) cells infiltrating the glomeruli. Necrotizing and crescentic glomerular lesions developed by day 7 with the increase of proteinuria. AT(1)R was expressed on CD8(+) and CD4(+) cells and on ED1(+) macrophages. Low-dose ARB had no anti-inflammatory effects in anti-GBM GN. However, high-dose ARB reduced glomerular infiltration of CD8(+) cells and ED1(+) macrophages and suppressed necrotizing and crescentic lesions by days 5 to 7 (P < 0.05). In addition, high-dose ARB reduced the numbers of ED3(+)-activated macrophages, suppressed glomerular TNF-alpha and IFN-gamma production, and downregulated M1-related chemokine and cytokines (monocyte chemoattractant protein type 1, IL-6, and IL-12). High-dose ARB also enhanced ED2(+) M2 macrophages by day 7 with upregulation of glomerular IL-4 and IL-13 and augmented CCL17, IL-1 receptor antagonist, and IL-10. We concluded that high-dose ARB inhibits glomerular inflammation by increasing the numbers of M2 macrophages and upregulation of anti-inflammatory cytokines and by suppressing M1 macrophage development with downregulation of M1-related proinflammatory cytokines. SN - 1522-1466 UR - https://www.unboundmedicine.com/medline/citation/20071465/ANG_II_receptor_blockade_enhances_anti_inflammatory_macrophages_in_anti_glomerular_basement_membrane_glomerulonephritis_ L2 - http://www.physiology.org/doi/full/10.1152/ajprenal.00374.2009?url_ver=Z39.88-2003&amp;rfr_id=ori:rid:crossref.org&amp;rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -