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Toll-like receptor expression on peripheral blood mononuclear cells in asthmatics; implications for asthma management.
J Clin Immunol 2010; 30(3):459-64JC

Abstract

BACKGROUND

Accumulating evidence indicates that cells expressing Toll-like receptors (TLRs) play an important role in allergic diseases. The authors undertook this study to explore the hypothesis that TLR-mediated inflammatory signals are important from the perspective of asthma management.

METHODS

The expressions of TLR1, TLR2, TLR3, TLR4, TLR6, and TLR9 and levels of pro-inflammatory cytokines (TNF-alpha, IL-1beta, IL-6, IL-8, and IFN-gamma) on the peripheral blood mononuclear cells (PBMCs) of 36 stable asthmatics on treatment (the on-treatment group), 15 asthmatics (the treatment-naïve group) before and after a 7-day course of oral prednisolone (30 mg/day), and on the PBMCs of 15 healthy controls were measured after in vitro stimulation using TLR-specific ligands.

RESULTS

In the on-treatment group, TLR1, TLR2, TLR6, and TLR9 expressions on PBMCs were significantly different between asthmatics and controls. And the expression of TLR4 on PBMCs and TNF-alpha production stimulated by lipopolysaccharide (LPS), were significantly higher in mild to moderate than in severe asthmatics. Interestingly, in the treatment-naïve group, short-term prednisolone significantly increased LPS-induced TNF-alpha and IFN-gamma productions by PBMCs.

CONCLUSION

TLR-mediated inflammatory signals contribute to the development and severity of asthma and are not reduced by glucocorticoid treatment, which suggests that a TLR-specific antagonist and glucocorticoid are required for the effective control of airway inflammation in asthmatics.

Authors+Show Affiliations

Department of Internal Medicine, Seoul National University, 28 Yongondong, Chongno-gu, Seoul, 110-744, Republic of Korea.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20072849

Citation

Chun, Eunyoung, et al. "Toll-like Receptor Expression On Peripheral Blood Mononuclear Cells in Asthmatics; Implications for Asthma Management." Journal of Clinical Immunology, vol. 30, no. 3, 2010, pp. 459-64.
Chun E, Lee SH, Lee SY, et al. Toll-like receptor expression on peripheral blood mononuclear cells in asthmatics; implications for asthma management. J Clin Immunol. 2010;30(3):459-64.
Chun, E., Lee, S. H., Lee, S. Y., Shim, E. J., Cho, S. H., Min, K. U., ... Park, H. W. (2010). Toll-like receptor expression on peripheral blood mononuclear cells in asthmatics; implications for asthma management. Journal of Clinical Immunology, 30(3), pp. 459-64. doi:10.1007/s10875-009-9363-z.
Chun E, et al. Toll-like Receptor Expression On Peripheral Blood Mononuclear Cells in Asthmatics; Implications for Asthma Management. J Clin Immunol. 2010;30(3):459-64. PubMed PMID: 20072849.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Toll-like receptor expression on peripheral blood mononuclear cells in asthmatics; implications for asthma management. AU - Chun,Eunyoung, AU - Lee,So-Hee, AU - Lee,Soo-Yeon, AU - Shim,Eun-Jin, AU - Cho,Sang-Heon, AU - Min,Kyung-Up, AU - Kim,You-Young, AU - Park,Heung-Woo, Y1 - 2010/01/14/ PY - 2009/10/30/received PY - 2009/12/10/accepted PY - 2010/1/15/entrez PY - 2010/1/15/pubmed PY - 2010/9/24/medline SP - 459 EP - 64 JF - Journal of clinical immunology JO - J. Clin. Immunol. VL - 30 IS - 3 N2 - BACKGROUND: Accumulating evidence indicates that cells expressing Toll-like receptors (TLRs) play an important role in allergic diseases. The authors undertook this study to explore the hypothesis that TLR-mediated inflammatory signals are important from the perspective of asthma management. METHODS: The expressions of TLR1, TLR2, TLR3, TLR4, TLR6, and TLR9 and levels of pro-inflammatory cytokines (TNF-alpha, IL-1beta, IL-6, IL-8, and IFN-gamma) on the peripheral blood mononuclear cells (PBMCs) of 36 stable asthmatics on treatment (the on-treatment group), 15 asthmatics (the treatment-naïve group) before and after a 7-day course of oral prednisolone (30 mg/day), and on the PBMCs of 15 healthy controls were measured after in vitro stimulation using TLR-specific ligands. RESULTS: In the on-treatment group, TLR1, TLR2, TLR6, and TLR9 expressions on PBMCs were significantly different between asthmatics and controls. And the expression of TLR4 on PBMCs and TNF-alpha production stimulated by lipopolysaccharide (LPS), were significantly higher in mild to moderate than in severe asthmatics. Interestingly, in the treatment-naïve group, short-term prednisolone significantly increased LPS-induced TNF-alpha and IFN-gamma productions by PBMCs. CONCLUSION: TLR-mediated inflammatory signals contribute to the development and severity of asthma and are not reduced by glucocorticoid treatment, which suggests that a TLR-specific antagonist and glucocorticoid are required for the effective control of airway inflammation in asthmatics. SN - 1573-2592 UR - https://www.unboundmedicine.com/medline/citation/20072849/Toll_like_receptor_expression_on_peripheral_blood_mononuclear_cells_in_asthmatics L2 - https://doi.org/10.1007/s10875-009-9363-z DB - PRIME DP - Unbound Medicine ER -